Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,140,294 T→C S9G (AGC→GGC)  hybF ← protein involved with the maturation of hydrogenases 1 and 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,140,2940TC100.0% 113.6 / NA 32S9G (AGC→GGC) hybFprotein involved with the maturation of hydrogenases 1 and 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/17);  total (15/17)

TGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGG  >  NC_000913/3140161‑3140425
                                                                                                                                     |                                                                                                                                   
tGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGc                                                                                                                                <  1:436144/139‑1 (MQ=255)
  gCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCaa                                                                                                                              <  1:325272/139‑1 (MQ=255)
        aaTTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGAc                                                                                                                        <  2:1142149/139‑1 (MQ=255)
                     aaaCGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGc                                                                                                                                >  1:359039/1‑118 (MQ=255)
                     aaaCGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGc                                                                                                                                <  2:359039/118‑1 (MQ=255)
                                  tctcCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGc                                                                                              <  1:84693/139‑1 (MQ=255)
                                       tcAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCa                                                                                         <  1:657755/139‑1 (MQ=255)
                                           cGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCaaa                                                                                                                             <  2:158319/99‑1 (MQ=255)
                                           cGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCaaa                                                                                                                             >  1:158319/1‑99 (MQ=255)
                                              aGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAAcagca                                                                                  >  2:55964/1‑139 (MQ=255)
                                                       cgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGAc                                                                         >  1:679080/1‑139 (MQ=255)
                                                                     cacaCGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGt                                                           <  1:55964/139‑1 (MQ=255)
                                                                       cacGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTg                                                                                               <  1:614562/101‑1 (MQ=255)
                                                                       cacGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTg                                                                                               >  2:614562/1‑101 (MQ=255)
                                                                                      cTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAAcag                                                                                    <  2:1053462/97‑1 (MQ=255)
                                                                                      cTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAAcag                                                                                    >  1:1053462/1‑97 (MQ=255)
                                                                                             tCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGc                                                                                                                                >  1:878116/1‑46 (MQ=255)
                                                                                             tCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGc                                                                                                                                <  2:878116/46‑1 (MQ=255)
                                                                                               gTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGa                                 >  2:404014/1‑139 (MQ=255)
                                                                                                 gctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGaaa                               >  2:256989/1‑139 (MQ=255)
                                                                                                     ctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATcc                                                     <  1:1280945/113‑1 (MQ=255)
                                                                                                     ctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATcc                                                     >  2:1280945/1‑113 (MQ=255)
                                                                                                        cGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGcgcg                                                                           <  2:216872/88‑1 (MQ=255)
                                                                                                        cGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGcgcg                                                                           >  1:216872/1‑88 (MQ=255)
                                                                                                            tGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGt                    <  1:256989/139‑1 (MQ=255)
                                                                                                                    gATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGa                                                                                            >  1:806032/1‑59 (MQ=255)
                                                                                                                    gATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGa                                                                                            <  2:806032/59‑1 (MQ=255)
                                                                                                                          ttCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGt      <  2:679080/139‑1 (MQ=255)
                                                                                                                              aCGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAgg  >  1:386268/1‑139 (MQ=255)
                                                                                                                              aCGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAgg  >  2:966452/1‑139 (MQ=255)
                                                                                                                                ggCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTcc                                                                                                 >  2:1282086/1‑42 (MQ=255)
                                                                                                                                ggCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTcc                                                                                                 <  1:1282086/42‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
TGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGG  >  NC_000913/3140161‑3140425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: