Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,151,425	G→A	A187V (GCA→GTA)	exbB ←	membrane spanning protein in TonB‑ExbB‑ExbD complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,151,425	0	G	A	100.0%	175.9 / NA	55	A187V (GCA→GTA)	exbB	membrane spanning protein in TonB‑ExbB‑ExbD complex
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/27);  total (28/27)

TGGCTTCCAGATCCAGGTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAG  >  NC_000913/3151300‑3151552                                                                                                                              |                                                                                                                                tGGCTTCCAGATCCAGGTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGAt                                                                                                                    <  2:589441/139‑1 (MQ=255) tGGCTTCCAGATCCAGGTCACGGATTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGAt                                                                                                                    <  1:598824/139‑1 (MQ=255)   gCTTCCAGATCCAGGTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTg                                                                                                                  <  1:1064135/139‑1 (MQ=255)     ttCCAGATCCAGGTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGcc                                                                                                                <  2:915508/139‑1 (MQ=255)             ccAGGTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTaa                                                                                                        >  2:247655/1‑139 (MQ=255)                ggTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATAGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACag                                                                                                     >  2:118943/1‑139 (MQ=255)                 gTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTaa                                                                                                        <  2:503758/135‑1 (MQ=255)                 gTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTaa                                                                                                        >  1:503758/1‑135 (MQ=255)                 gTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACaga                                                                                                    <  2:844843/139‑1 (MQ=255)                  tCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACagag                                                                                                   >  2:140310/1‑139 (MQ=255)                      ggCTTTGCAGCAACATTACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAATGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTc                                                                                               >  2:865997/1‑139 (MQ=255)                      ggCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTc                                                                                               >  1:1084979/1‑139 (MQ=255)                         tttGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGc                                                                                            <  1:247655/139‑1 (MQ=255)                                caacaaTACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATAccc                                                                                     >  1:1202355/1‑139 (MQ=255)                                     aTACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTCTAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGgcgc                                                                                <  1:865997/139‑1 (MQ=255)                                      tACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCa                                                                               >  1:316162/1‑139 (MQ=255)                                              cTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGc                                                                       <  2:4525/139‑1 (MQ=255)                                                 cAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAg                                                                    >  1:1272772/1‑139 (MQ=255)                                                      tCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAg                                                               <  1:1181874/139‑1 (MQ=255)                                                              cATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGc                                                       >  1:745387/1‑139 (MQ=255)                                                                   cTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCa                                                                     >  1:64617/1‑120 (MQ=255)                                                                   cTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCa                                                                     <  2:64617/120‑1 (MQ=255)                                                                     ttAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTgcg                                                <  1:1117061/139‑1 (MQ=255)                                                                           ccgccAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTg                                                                                                                  >  1:124081/1‑67 (MQ=255)                                                                           ccgccAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTg                                                                                                                  <  2:124081/67‑1 (MQ=255)                                                                            cgccAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTg                                                                                                                  <  2:185164/66‑1 (MQ=255)                                                                            cgccAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTg                                                                                                                  >  1:185164/1‑66 (MQ=255)                                                                             gccAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCa                                        >  2:987956/1‑139 (MQ=255)                                                                              ccAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGt                                                           <  1:916451/119‑1 (MQ=255)                                                                              ccAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGt                                                           >  2:916451/1‑119 (MQ=255)                                                                                         tGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTgcg                                                <  1:842064/119‑1 (MQ=255)                                                                                         tGCAAATACGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTgcg                                                >  2:842064/1‑119 (MQ=255)                                                                                                aCGTTATAGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGc                     >  2:1203885/1‑139 (MQ=255)                                                                                                    tataGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGc                                                                       >  1:17298/1‑85 (MQ=255)                                                                                                    tataGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGc                                                                       <  2:17298/85‑1 (MQ=255)                                                                                                    tataGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCa                 >  1:70909/1‑139 (MQ=255)                                                                                                    tataGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCa                 <  2:316162/139‑1 (MQ=255)                                                                                                      taGATAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGa               <  2:1202355/139‑1 (MQ=255)                                                                                                         aTAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCatga                        >  1:1003499/1‑127 (MQ=255)                                                                                                         aTAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCatga                        <  2:1003499/127‑1 (MQ=255)                                                                                                         aTAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCatg                         <  2:306239/126‑1 (MQ=255)                                                                                                         aTAACGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCatg                         >  1:306239/1‑126 (MQ=255)                                                                                                           aaCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACag                                                                                                     >  1:783939/1‑48 (MQ=255)                                                                                                           aaCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACag                                                                                                     <  2:783939/48‑1 (MQ=255)                                                                                                           aaCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCtt                                                                                                >  1:631555/1‑53 (MQ=255)                                                                                                           aaCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCtt                                                                                                <  2:631555/53‑1 (MQ=255)                                                                                                            aCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGt                                                                                                              <  1:129413/38‑1 (MQ=255)                                                                                                            aCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGt                                                                                                              >  2:129413/1‑38 (MQ=255)                                                                                                            aCGACCGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTAc         >  1:912412/1‑139 (MQ=255)                                                                                                                ccGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGc                                                                       >  2:78987/1‑73 (MQ=255)                                                                                                                ccGCAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGc                                                                       <  1:78987/73‑1 (MQ=255)                                                                                                                   cAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGt                                                           >  2:966715/1‑82 (MQ=255)                                                                                                                   cAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGt                                                           <  1:966715/82‑1 (MQ=255)                                                                                                                   cAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAg  <  2:745387/139‑1 (MQ=255)                                                                                                                   cAGGAATCGCTACCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAg  >  1:14474/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                TGGCTTCCAGATCCAGGTCACGGCTTTGCAGCAACAATACCTGCGCTGCAACATCACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAG  >  NC_000913/3151300‑3151552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑