Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,178,015	G→A	intergenic (‑105/‑100)	nudF ← / → tolC	ADP‑ribose pyrophosphatase/transport channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,178,015	0	G	A	100.0%	114.5 / NA	36	intergenic (‑105/‑100)	nudF/tolC	ADP‑ribose pyrophosphatase/transport channel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/15);  total (21/15)

AGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAG  >  NC_000913/3177891‑3178149                                                                                                                             |                                                                                                                                       aGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAAc                                                                                                                          >  2:67543/1‑139 (MQ=255)             ttAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCtt                                                                                                                       >  2:934358/1‑130 (MQ=255)             ttAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCtt                                                                                                                       <  1:934358/130‑1 (MQ=255)             ttAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAg                                                                                                              >  2:681017/1‑139 (MQ=255)                            gTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          >  1:1065264/1‑128 (MQ=255)                            gTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          <  2:1065264/128‑1 (MQ=255)                                 ggCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          <  2:346224/123‑1 (MQ=255)                                 ggCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          >  1:346224/1‑123 (MQ=255)                                 ggCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGc                                                                                          >  1:513485/1‑139 (MQ=255)                                 ggCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGACAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGc                                                                                          >  1:213082/1‑139 (MQ=255)                                             gAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          >  1:1046156/1‑111 (MQ=255)                                             gAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          <  2:1046156/111‑1 (MQ=255)                                              aaTTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAg                                                                             >  1:977783/1‑139 (MQ=255)                                                ttAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          >  2:963948/1‑108 (MQ=255)                                                ttAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          <  1:963948/108‑1 (MQ=255)                                                ttAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGtt                                                                           >  2:448749/1‑139 (MQ=255)                                                   aaTGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGa                                                                        <  1:681017/139‑1 (MQ=255)                                                     tgtgAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATc                                                                      <  1:1077977/139‑1 (MQ=255)                                                      gtgAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATcg                                                                     >  2:671245/1‑139 (MQ=255)                                                          aTTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCt                                                                 >  1:411468/1‑139 (MQ=255)                                                               aGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATa                                                            >  1:31417/1‑139 (MQ=255)                                                                    cGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCt                                                       <  1:448749/139‑1 (MQ=255)                                                                                   tGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATg                                        <  1:763893/139‑1 (MQ=255)                                                                                     aGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCa                                      <  2:31417/139‑1 (MQ=255)                                                                                                      ttGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          >  1:104498/1‑54 (MQ=255)                                                                                                      ttGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          <  2:104498/54‑1 (MQ=255)                                                                                                      ttGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTcc                     >  1:476047/1‑139 (MQ=255)                                                                                                        gAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTa                                                                                <  1:939446/78‑1 (MQ=255)                                                                                                        gAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTa                                                                                >  2:939446/1‑78 (MQ=255)                                                                                                         aGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCa                  >  1:799947/1‑139 (MQ=255)                                                                                                           gCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATc                                                                      >  2:133244/1‑85 (MQ=255)                                                                                                           gCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATc                                                                      <  1:133244/85‑1 (MQ=255)                                                                                                                        cTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGa   >  2:140413/1‑139 (MQ=255)                                                                                                                         tATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATc                                                                      <  2:1218877/71‑1 (MQ=255)                                                                                                                         tATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATc                                                                      >  1:1218877/1‑71 (MQ=255)                                                                                                                         tATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAg  <  2:411468/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                       AGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAG  >  NC_000913/3177891‑3178149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑