| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,199,429 | T→C | A71A (GCA→GCG) | glnE ← | fused deadenylyltransferase/adenylyltransferase for glutamine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,199,429 | 0 | T | C | 98.3% | 206.4 / ‑4.8 | 59 | A71A (GCA→GCG) | glnE | fused deadenylyltransferase/adenylyltransferase for glutamine synthetase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (30/28); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
TCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCATGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCG > NC_000913/3199302‑3199555 | tctctTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCATGCCGCGTAAtg < 1:1013117/139‑1 (MQ=255)tctctTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAAtg < 2:92252/139‑1 (MQ=255)tctctTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAAtg > 1:101026/1‑139 (MQ=255) tAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATtc > 2:1065908/1‑139 (MQ=255) aGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCgg > 1:994152/1‑139 (MQ=255) aGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCgg > 1:900671/1‑139 (MQ=255) tGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGcc > 2:431921/1‑139 (MQ=255) ccAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTg > 1:562309/1‑139 (MQ=255) gTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTgcggcg > 2:965177/1‑139 (MQ=255) ttGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGt < 1:586474/139‑1 (MQ=255) gcACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGt < 1:424425/139‑1 (MQ=255) aCCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCa > 2:21888/1‑139 (MQ=255) cATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGc < 2:101026/139‑1 (MQ=255) cATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGc < 1:1065908/139‑1 (MQ=255) cgccgGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTgg > 2:735423/1‑111 (MQ=255) cgccgGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTgg < 1:735423/111‑1 (MQ=255) cgccgGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGAt > 1:47686/1‑139 (MQ=255) cgccgGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGAt > 2:1280810/1‑139 (MQ=255) cgGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATgcg < 2:900671/139‑1 (MQ=255) ccGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg < 1:431921/139‑1 (MQ=255) ccGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg < 2:47686/139‑1 (MQ=255) ccGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg < 2:729402/139‑1 (MQ=255) ccGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg < 1:754464/139‑1 (MQ=255) ccGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg < 1:1280810/139‑1 (MQ=255) gCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTcc < 1:728511/101‑1 (MQ=255) gCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTcc > 2:728511/1‑101 (MQ=255) cTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTcc < 2:562309/139‑1 (MQ=255) cgcATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGca > 1:1153269/1‑139 (MQ=255) aaCCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGca < 1:125065/133‑1 (MQ=255) aaCCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGca > 2:125065/1‑133 (MQ=255) cGTCACTCACATTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAaagt < 1:21888/139‑1 (MQ=255) cacaTTACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACt > 2:545060/1‑139 (MQ=255) tACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGca < 2:1210564/111‑1 (MQ=255) tACAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGca > 1:1210564/1‑111 (MQ=255) aCAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTgcggcg < 1:204218/56‑1 (MQ=255) aCAGAGCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTgcggcg > 2:204218/1‑56 (MQ=255) gagCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTgg < 1:1141838/58‑1 (MQ=255) gagCGCCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTgg > 2:1141838/1‑58 (MQ=255) gctcctcctGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCg > 2:748697/4‑69 (MQ=255) gcgcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCg < 1:748697/69‑1 (MQ=255) gcgcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAg > 2:747952/1‑73 (MQ=255) gcgcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAg < 1:747952/73‑1 (MQ=255) cgcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg > 2:828385/1‑94 (MQ=255) cgcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg < 1:828385/94‑1 (MQ=255) cgcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTa > 2:320311/1‑115 (MQ=255) cgcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTa < 1:320311/115‑1 (MQ=255) gcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg < 2:304997/93‑1 (MQ=255) gcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg < 2:297127/93‑1 (MQ=255) gcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg > 1:304997/1‑93 (MQ=255) gcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg > 1:297127/1‑93 (MQ=255) gcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCtgtg > 2:1096586/1‑139 (MQ=255) gcCTCCTGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCtgtg > 1:1216990/1‑139 (MQ=255) ctcctGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg < 1:853375/91‑1 (MQ=255) ctcctGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCtgtg < 2:183503/137‑1 (MQ=255) ctcctGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCtgtg > 1:183503/1‑137 (MQ=255) ctcctGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTgcg > 2:140204/1‑139 (MQ=255) ctcctGCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTGTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACg > 2:853375/1‑91 (MQ=255) gCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTc > 2:138077/1‑91 (MQ=255) gCAACCACGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTc < 1:138077/91‑1 (MQ=255) | TCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCATGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCG > NC_000913/3199302‑3199555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |