Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 284,869 C→T P557S (CCG→TCG)  yagF → CP4‑6 prophage, dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913284,8690CT100.0% 103.9 / NA 34P557S (CCG→TCG) yagFCP4‑6 prophage, dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/18);  total (16/18)

TACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGCCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGA  >  NC_000913/284737‑284993
                                                                                                                                    |                                                                                                                            
tACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTg                                                                                                                        >  2:693875/1‑139 (MQ=255)
 aCCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTgg                                                                                                                       >  2:1009853/1‑139 (MQ=255)
         aCCTCCGCGCTAAAGCATATCTCGT‑GGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTgcg                                                                                                              <  2:339378/139‑1 (MQ=255)
                  cTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACg                                                                                                      <  1:890387/139‑1 (MQ=255)
                    aaaGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGAc                                                                                                    <  2:272720/139‑1 (MQ=255)
                              tcGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                          <  1:1241071/139‑1 (MQ=255)
                              tcGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                          <  1:167058/139‑1 (MQ=255)
                              tcGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                          <  1:693875/139‑1 (MQ=255)
                               cGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCa                                                                                                                     <  1:1062816/111‑1 (MQ=255)
                               cGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCa                                                                                                                     >  2:1062816/1‑111 (MQ=255)
                                  ggggCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                          <  1:1073724/135‑1 (MQ=255)
                                  ggggCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                          >  2:1073724/1‑135 (MQ=255)
                                              tgtCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCt                                                                          >  1:148119/1‑139 (MQ=255)
                                                   cTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTgcg                                                                                                              >  2:735007/1‑98 (MQ=255)
                                                   cTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTgcg                                                                                                              <  1:735007/98‑1 (MQ=255)
                                                          ccGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTgg                                                              <  2:148119/139‑1 (MQ=255)
                                                                cgcgcTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTg                                                                                                                >  1:885214/1‑83 (MQ=255)
                                                                cgcgcTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTg                                                                                                                <  2:885214/83‑1 (MQ=255)
                                                                  cgcTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                          <  2:925866/103‑1 (MQ=255)
                                                                  cgcTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCgaga                                                                                          >  1:925866/1‑103 (MQ=255)
                                                                  cgcTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGa                                                      >  2:1230921/1‑139 (MQ=255)
                                                                               tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGt                                                                                   >  1:429270/1‑97 (MQ=255)
                                                                               tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGt                                                                                   <  2:429270/97‑1 (MQ=255)
                                                                               tgtCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCAc                                         >  2:1271830/1‑139 (MQ=255)
                                                                                 tCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCa                                                >  1:150664/1‑130 (MQ=255)
                                                                                 tCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCa                                                <  2:1166473/130‑1 (MQ=255)
                                                                                 tCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCa                                                <  2:150664/130‑1 (MQ=255)
                                                                                 tCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCa                                                >  1:1166473/1‑130 (MQ=255)
                                                                                      gggCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGAc                                  <  1:1271830/139‑1 (MQ=255)
                                                                                           ccTGCTTCGGCCACGTGTCGCCGGAGGAGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACGGCGGACAcccc                             >  1:838180/1‑139 (MQ=255)
                                                                                            cTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCg                            <  2:1177702/139‑1 (MQ=255)
                                                                                                         gtgtCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCg                                             >  1:945201/1‑109 (MQ=255)
                                                                                                         gtgtCGCCGGAGGCGCTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCg                                             <  2:945201/109‑1 (MQ=255)
                                                                                                                      cgcTGGCGGGCGGGTCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGCCAACCCGCTGACGCCGGAAGAGGGCGCGCGCGa  >  2:1217159/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                            
TACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGGCGTGTCGACGGGCGCCTGCTTCGGCCACGTGTCGCCGGAGGCGCTGGCGGGCGGGCCGATTGGCAAGCTGCGCGATAACGACATCATCGAGATTGCCGTGGATCGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGA  >  NC_000913/284737‑284993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑