Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 287,977 A→G M397V (ATG→GTG)  yagH → CP4‑6 prophage, putative xylosidase/arabinosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913287,9770AG100.0% 150.5 / NA 43M397V (ATG→GTG) yagHCP4‑6 prophage, putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/22);  total (21/22)

ACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCG  >  NC_000913/287843‑288106
                                                                                                                                      |                                                                                                                                 
acgacACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGa                                                                                                                               >  2:304618/1‑139 (MQ=255)
        cTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGc                                                                                                                       <  1:976903/139‑1 (MQ=255)
         tCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGTGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGcc                                                                                                                      >  2:873040/1‑139 (MQ=255)
                 ctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATAGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGAAGAAAAGCGGGTGGAGTTCTCGCCGGTGCACt                                                                                                              >  1:1111453/1‑139 (MQ=255)
                        cgcgcCCGGGCTTCTTACAGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGTGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCcagca                                                                                                       <  1:873040/139‑1 (MQ=255)
                            cccGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAgc                                                                                                   >  2:529594/1‑139 (MQ=255)
                                             tctATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGcta                                                                                  <  2:668418/139‑1 (MQ=255)
                                               tATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGctact                                                                                >  1:1154949/1‑139 (MQ=255)
                                               tATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGctact                                                                                >  1:772906/1‑139 (MQ=255)
                                                         aCTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGACTGACCTGCTACTACAACAGCaa                                                                      >  2:320290/1‑139 (MQ=255)
                                                              cTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACt                                                                 <  2:500904/139‑1 (MQ=255)
                                                                  aTTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAg                                                             <  2:1012608/139‑1 (MQ=255)
                                                                        ccTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGAGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTg                                                       >  2:645726/1‑139 (MQ=255)
                                                                        ccTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCGTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGGTACTg                                                       <  2:362934/139‑1 (MQ=255)
                                                                           tCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCgggg                                                                                             >  2:918645/1‑98 (MQ=255)
                                                                           tCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCgggg                                                                                             <  1:918645/98‑1 (MQ=255)
                                                                             aCCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAc                                                                                        <  1:113530/101‑1 (MQ=255)
                                                                             aCCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAc                                                                                        >  2:113530/1‑101 (MQ=255)
                                                                             aCCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTtg                                                  >  2:964488/1‑139 (MQ=255)
                                                                                  aTCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGAc                                             >  1:1178614/1‑139 (MQ=255)
                                                                                      aCCGTGGTGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCCGTGCACTTCCAGCAGAgcg                                                                                                  >  2:846631/1‑82 (MQ=255)
                                                                                      aCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCATAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAgcg                                                                                                  <  1:846631/82‑1 (MQ=255)
                                                                                      aCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCtactac                                                                              <  1:425938/102‑1 (MQ=255)
                                                                                      aCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCtactac                                                                              >  2:425938/1‑102 (MQ=255)
                                                                                      aCCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACg                                         >  1:680976/1‑139 (MQ=255)
                                                                                         gTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACgagg                                      <  2:262170/139‑1 (MQ=255)
                                                                                             cgcgcCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTtct                                                                                                                          <  1:43753/51‑1 (MQ=255)
                                                                                             cgcgcCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTtct                                                                                                                          >  2:43753/1‑51 (MQ=255)
                                                                                              gcgcCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGa                                 <  1:529594/139‑1 (MQ=255)
                                                                                              gcgcCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGa                                 <  2:1154949/139‑1 (MQ=255)
                                                                                                      ggCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAg                         <  1:964488/139‑1 (MQ=255)
                                                                                                            aCTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCAt                   <  1:154910/139‑1 (MQ=255)
                                                                                                            aCTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCAt                   <  2:772906/139‑1 (MQ=255)
                                                                                                            aCTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCAt                   <  2:1178614/139‑1 (MQ=255)
                                                                                                            aCTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGACTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCAt                   <  1:320290/139‑1 (MQ=255)
                                                                                                                cGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCggg                                                                                              <  1:1042038/60‑1 (MQ=255)
                                                                                                                cGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCggg                                                                                              >  2:1042038/1‑60 (MQ=255)
                                                                                                                cGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCaaa               >  1:250919/1‑139 (MQ=255)
                                                                                                                   cTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTATAActttgatggtt            <  1:304618/139‑11 (MQ=255)
                                                                                                                       cGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATcc        >  2:168140/1‑139 (MQ=255)
                                                                                                                             gAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCg  >  1:392723/1‑139 (MQ=255)
                                                                                                                                aCGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACgagg                                      >  2:71475/1‑100 (MQ=255)
                                                                                                                                aCGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACgagg                                      <  1:71475/100‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                 
ACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCG  >  NC_000913/287843‑288106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑