Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	291,324	C→T	C8Y (TGT→TAT)	insA ←	IS1 repressor TnpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	291,324	0	C	T	100.0%	84.4 / NA	29	C8Y (TGT→TAT)	insA	IS1 repressor TnpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/13);  total (16/13)

TGTCCGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGATAAGGT  >  NC_000913/291267‑291456                                                          |                                                                                                                                     tGTCCGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAAt                                                     >  2:1005099/1‑139 (MQ=14)     cGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTc                                                 >  2:1149598/1‑139 (MQ=21)          gTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGGGGGATAGCTGGTAGAAACAGAAGCCACTGGAGCACCTCAATAACACCATCATACACTAGATCAGTAAGTTGGCATCATCACCGAATTTTCAGccc                                            <  2:1002334/139‑1 (MQ=14)               tttGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATg                                       <  1:875928/139‑1 (MQ=255)                  gCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGttt                                    >  2:1273792/1‑139 (MQ=255)                    cGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCa                                  <  2:545048/139‑1 (MQ=255)                    cGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCa                                  <  1:1287164/139‑1 (MQ=255)                         cGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTAc                             <  1:1149598/139‑1 (MQ=255)                              caccCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTa                          >  2:848321/1‑137 (MQ=255)                              caccCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTa                          <  1:848321/137‑1 (MQ=255)                                ccccGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTTAAAAACACCATCATACACTAAATCAGAAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTc                      >  2:708495/1‑139 (MQ=255)                                ccccGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCgg                                          <  1:378461/119‑1 (MQ=255)                                ccccGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCgg                                          >  2:378461/1‑119 (MQ=255)                                ccccGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCt                           >  1:938463/1‑134 (MQ=255)                                ccccGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCt                           <  2:938463/134‑1 (MQ=255)                                ccccGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTc                      >  2:873713/1‑139 (MQ=255)                                ccccGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTc                      >  2:498887/1‑139 (MQ=255)                                ccccGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTC‑AAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCg                     >  1:157793/1‑139 (MQ=255)                                 cccGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCg                     >  2:1013734/1‑139 (MQ=255)                                   cGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCt                           >  2:371807/1‑131 (MQ=255)                                   cGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCt                           <  1:371807/131‑1 (MQ=255)                                   cGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGaa                   >  2:1224101/1‑139 (MQ=255)                                              gAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGa        <  1:708495/139‑1 (MQ=255)                                              gAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGa        <  1:498887/139‑1 (MQ=255)                                               aaCAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGAt       >  1:320941/1‑139 (MQ=255)                                                aCAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGATa      <  2:1126618/139‑1 (MQ=255)                                                    gaggGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCAGATAAGGt  >  2:1106635/1‑139 (MQ=255)                                                     aggGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTc                      <  1:99844/118‑1 (MQ=255)                                                     aggGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTc                      >  2:99844/1‑118 (MQ=255)                                                          |                                                                                                                                     TGTCCGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGATAAGGT  >  NC_000913/291267‑291456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑