Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,400,093	G→A	A332V (GCG→GTG)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,400,093	0	G	A	100.0%	174.9 / NA	54	A332V (GCG→GTG)	mreB	cell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/25);  total (29/25)

GGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTC  >  NC_000913/3399971‑3400226                                                                                                                           |                                                                                                                                      ggCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcg                                                                                                                       <  1:479044/139‑1 (MQ=255)                      tctcGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTc                                                                                                  >  2:197064/1‑139 (MQ=255)                      tctcGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTc                                                                                                  >  1:1160596/1‑139 (MQ=255)                      tctcGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTACGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTc                                                                                                  <  2:47153/139‑1 (MQ=255)                         cGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTc                                                                                               >  2:1107192/1‑139 (MQ=255)                          gTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcg                                                                                                                       >  2:93795/1‑114 (MQ=255)                          gTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcg                                                                                                                       <  1:93795/114‑1 (MQ=255)                             tCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGca                                                                                           >  1:39872/1‑139 (MQ=255)                               aGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGcaac                                                                                         <  2:670063/139‑1 (MQ=255)                                 aCCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTgcc                                                                                                                                <  1:1218379/98‑1 (MQ=255)                                 aCCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTgcc                                                                                                                                >  2:1218379/1‑98 (MQ=255)                                 aCCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGcaacaa                                                                                       >  2:832012/1‑139 (MQ=255)                                     ggcaggGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGAc                                                                                   >  2:660192/1‑139 (MQ=255)                                        aggGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTgg                                                                                <  2:1160596/139‑1 (MQ=255)                                           gTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTTGCCGCCGTGCATGTCGATCATGTCTAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCGACAACGACTGGAAt                                                                             >  1:793765/1‑139 (MQ=255)                                           gTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCgg                                                                                                     >  1:226797/1‑115 (MQ=255)                                           gTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCgg                                                                                                     <  2:226797/115‑1 (MQ=255)                                                    cacTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTttc                                                                    >  2:1233450/1‑139 (MQ=255)                                                       ttCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTttcttc                                                                 <  1:832012/139‑1 (MQ=255)                                                          cccTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCAt                                                              >  2:668529/1‑139 (MQ=255)                                                                   cATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCtt                                                                                                >  2:1179620/1‑96 (MQ=255)                                                                   cATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCtt                                                                                                <  1:1179620/96‑1 (MQ=255)                                                                   cATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTccc                                                               <  2:807227/129‑2 (MQ=255)                                                                   cATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCa                                                               >  1:807227/1‑129 (MQ=255)                                                                   cATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACg                                                     >  2:1075539/1‑139 (MQ=255)                                                                     tCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGt                                                   <  1:1075539/139‑1 (MQ=255)                                                                        gATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCaa                                                <  1:445555/139‑1 (MQ=255)                                                                         aTTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAg                                               >  2:564907/1‑139 (MQ=255)                                                                               ctTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACg                                         <  1:564907/139‑1 (MQ=255)                                                                                  cGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACgcag                                      <  1:660192/139‑1 (MQ=255)                                                                                   gCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACgcagc                                     <  1:1107192/139‑1 (MQ=255)                                                                                     tGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCAGACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGcagcag                                   <  2:793765/139‑1 (MQ=255)                                                                                       aaCAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTg                                 >  2:1243030/1‑139 (MQ=255)                                                                                            gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCAcc                                                                                                                          <  1:838315/45‑1 (MQ=255)                                                                                            gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCAcc                                                                                                                          >  2:838315/1‑45 (MQ=255)                                                                                            gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcgcg                                                                                                                     <  1:1041475/50‑1 (MQ=255)                                                                                            gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcgcg                                                                                                                     >  2:1041475/1‑50 (MQ=255)                                                                                            gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCgg                                                                                                     >  2:443515/1‑66 (MQ=255)                                                                                            gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCgg                                                                                                     <  1:443515/66‑1 (MQ=255)                                                                                            gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATg                                                                            >  2:1189237/1‑91 (MQ=255)                                                                                            gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATg                                                                            <  1:1189237/91‑1 (MQ=255)                                                                                            gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTcc                                                                <  1:1016351/103‑1 (MQ=255)                                                                                            gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTcc                                                                >  2:1016351/1‑103 (MQ=255)                                                                                            gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGcagcag                                   >  1:543519/1‑132 (MQ=255)                                                                                            gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGcagcag                                   <  2:543519/132‑1 (MQ=255)                                                                                            gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGcca                            >  2:869405/1‑139 (MQ=255)                                                                                            gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGcca                            >  2:420494/1‑139 (MQ=255)                                                                                            gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGC‑‑CACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGccacc                          >  2:56566/1‑139 (MQ=255)                                                                                               gccgccGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTttctt                                                                  >  2:1296196/1‑98 (MQ=255)                                                                                               gccgccGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTttctt                                                                  <  1:1296196/98‑1 (MQ=255)                                                                                                                tCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGcaac                                                                                         >  1:1200577/1‑58 (MQ=255)                                                                                                                tCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGcaac                                                                                         <  2:1200577/58‑1 (MQ=255)                                                                                                                 cATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCcg       <  2:39872/139‑1 (MQ=255)                                                                                                                      ccAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTc  >  1:1010821/1‑139 (MQ=255)                                                                                                                           |                                                                                                                                      GGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTC  >  NC_000913/3399971‑3400226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑