| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,410,406 | T→C | S43P (TCT→CCT) | dusB → | tRNA‑dihydrouridine synthase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,410,406 | 0 | T | C | 98.6% | 253.3 / NA | 72 | S43P (TCT→CCT) | dusB | tRNA‑dihydrouridine synthase B |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (38/33); minor base A (0/1); total (38/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGC > NC_000913/3410273‑3410527 | ttAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGccttct < 1:488065/139‑1 (MQ=255) tCATTCGCAGCGGAAAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGACATTACAGATAGACCGTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGACTTCTAAccc < 2:1131961/139‑1 (MQ=255) cATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCca < 1:1177953/139‑1 (MQ=255) aTGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCcac < 1:732774/139‑1 (MQ=255) aTGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCcac > 2:732774/1‑139 (MQ=255) aTGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCcac > 2:253904/1‑139 (MQ=255) tCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCca > 2:140004/1‑131 (MQ=255) tCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCca < 1:140004/131‑1 (MQ=255) cGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTgg > 1:387002/1‑139 (MQ=255) cGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTgg > 1:1248610/1‑139 (MQ=255) cAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGaaa < 1:537850/139‑1 (MQ=255) cAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACa > 2:160183/1‑139 (MQ=255) cTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAAt > 1:255441/1‑139 (MQ=255) cAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAAtct > 1:791846/1‑139 (MQ=255) cAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAAtct > 1:465382/1‑139 (MQ=255) gAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCcac < 2:533955/114‑1 (MQ=255) gAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCcac > 1:533955/1‑114 (MQ=255) tCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTa < 2:596002/139‑1 (MQ=255) ccTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACgg < 1:949794/139‑1 (MQ=255) cTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGa < 2:255441/139‑1 (MQ=255) cTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGa < 1:746030/139‑1 (MQ=255) aTCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGccttc > 1:993732/1‑95 (MQ=255) aTCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGccttc < 2:993732/95‑1 (MQ=255) aTCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATgg > 2:608926/1‑139 (MQ=255) cAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGca < 1:1231644/139‑1 (MQ=255) ccATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAAt > 1:93246/1‑114 (MQ=255) ccATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAAt < 2:93246/114‑1 (MQ=255) aTGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGccttc > 2:414792/1‑83 (MQ=255) aTGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGccttc < 1:414792/83‑1 (MQ=255) tggctggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATtgatga < 1:608926/139‑1 (MQ=255) ggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCca < 1:833965/84‑1 (MQ=255) ggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCca > 2:833965/1‑84 (MQ=255) gcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCgg > 2:785162/1‑139 (MQ=255) gacagacCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCa < 2:1248610/139‑1 (MQ=255) acagacCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAc > 2:1039149/1‑139 (MQ=255) cagacCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAcc > 2:1068088/1‑139 (MQ=255) agacCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGa < 2:851551/106‑1 (MQ=255) agacCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGa > 1:851551/1‑106 (MQ=255) gacCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGt > 1:343440/1‑139 (MQ=255) acCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCAGGCATTCGCACCGtt > 2:105120/1‑138 (MQ=255) ccTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGc > 1:903959/1‑139 (MQ=255) ttCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCca > 2:1086672/1‑65 (MQ=255) ttCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCca < 1:1086672/65‑1 (MQ=255) ttCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAAt > 1:1153082/1‑139 (MQ=255) aCGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTg > 1:443864/1‑99 (MQ=255) aCGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTg < 2:443864/99‑1 (MQ=255) tgtgCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAg < 1:1068088/139‑1 (MQ=255) gtgCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCca > 1:69941/1‑55 (MQ=255) gtgCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCca < 2:69941/55‑1 (MQ=255) gtgCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGc > 2:201743/1‑139 (MQ=255) tgCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCg < 1:785162/139‑1 (MQ=255) agaTGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCaaa > 1:329441/1‑120 (MQ=255) agaTGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCaaa < 2:329441/120‑1 (MQ=255) gaTGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGa < 2:1117127/80‑1 (MQ=255) gaTGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGa > 1:1117127/1‑80 (MQ=255) tGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCca < 1:186149/44‑1 (MQ=255) tGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCca > 2:186149/1‑44 (MQ=255) tGGGAGCCGGATTGACAGCATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGc > 1:416816/1‑59 (MQ=255) tGGGAGCCGGATTGACAGCATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGc < 2:416816/59‑1 (MQ=255) gggAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATGCgaaagaa > 1:768670/1‑139 (MQ=255) gAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAAt > 2:1042934/1‑139 (MQ=255) gCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATgg > 1:1155895/1‑139 (MQ=255) gATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGc < 2:725726/136‑1 (MQ=255) gATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGc > 1:725726/1‑136 (MQ=255) cAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATgcagc < 2:387002/139‑1 (MQ=255) cAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATgcagc < 2:903959/139‑1 (MQ=255) cAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATgcagc < 1:1042934/139‑1 (MQ=255) aTCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGCTGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATgcagc < 2:928987/135‑1 (MQ=255) aTCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATgcagc > 1:928987/1‑135 (MQ=255) tCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTAc > 1:1095284/1‑55 (MQ=255) tCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTAc < 2:1095284/55‑1 (MQ=255) tCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc < 2:1017929/78‑1 (MQ=255) tCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc > 1:1017929/1‑78 (MQ=255) gatgCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCg > 2:1047673/1‑42 (MQ=255) gatgCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCg < 1:1047673/42‑1 (MQ=255) atgCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGca > 2:576681/1‑56 (MQ=255) atgCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGca < 1:576681/56‑1 (MQ=255) | TTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGC > NC_000913/3410273‑3410527 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |