Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,478,802	C→T	*602* (TAG→TAA)	kefB ←	potassium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,478,802	0	C	T	100.0%	112.0 / NA	37	*602* (TAG→TAA)	kefB	potassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/20);  total (17/20)

TGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCA  >  NC_000913/3478674‑3478923                                                                                                                                 |                                                                                                                          tGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAAtt                                                                                                                 <  1:624723/139‑1 (MQ=255)     cGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATc                                                                                                             >  2:244802/1‑139 (MQ=255)      gCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATcc                                                                                                            <  2:803126/139‑1 (MQ=255)            aaCACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCg                                                                                                      >  2:514167/1‑139 (MQ=255)                      acaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGc                                                                                            <  1:1188219/139‑1 (MQ=255)                      acaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTCACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGc                                                                                            >  2:757541/1‑139 (MQ=255)                          tatcaatatTATTTTCGCGCCACATCGCCCTTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGcgtcg                                                                                        >  2:796695/1‑139 (MQ=255)                          tatcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCTGTCCAGCTGGcgtcg                                                                                        >  1:187276/1‑139 (MQ=255)                           atcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGcgtcgt                                                                                       <  1:244802/139‑1 (MQ=255)                               atatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTc                                                                                     <  1:221605/137‑1 (MQ=255)                               atatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTc                                                                                     >  2:221605/1‑137 (MQ=255)                               atatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCtt                                                                                   >  1:1121938/1‑139 (MQ=255)                                tattatTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttg                                                                                  <  2:144242/139‑1 (MQ=255)                                     ttttCGCGCCACATCGCCATTGAATCCTGCGCCTTACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCa                                                                             >  2:744457/1‑139 (MQ=255)                                             ccACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGc                                                                     <  1:514167/139‑1 (MQ=255)                                                   cGCCATTGAATCCTGCGCCTTACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGaaa                                                               <  1:744457/139‑1 (MQ=255)                                                        ttGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCa                                                                                                          <  1:708238/91‑1 (MQ=255)                                                        ttGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCa                                                                                                          >  2:708238/1‑91 (MQ=255)                                                             tCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCa                                                     >  2:247271/1‑139 (MQ=255)                                                                  cgcCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTcg                                                >  2:1050960/1‑139 (MQ=255)                                                                       gACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCg                                           >  1:868273/1‑139 (MQ=255)                                                                         cAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCggg                                         <  2:1185243/139‑1 (MQ=255)                                                                         cAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCggg                                         <  1:579707/139‑1 (MQ=255)                                                                          aGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGc                                        <  2:1121938/139‑1 (MQ=255)                                                                            gCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGcgtcg                                                                                        >  2:416606/1‑89 (MQ=255)                                                                            gCCGGACATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGcgtcg                                                                                        <  1:416606/89‑1 (MQ=255)                                                                                 ggaTTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTg                                 >  2:776877/3‑139 (MQ=255)                                                                                           cccGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCAAGaaa                       >  2:497664/1‑139 (MQ=255)                                                                                                 aTAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCAAGAAATTTGTa                 <  1:497664/139‑1 (MQ=255)                                                                                                    aaaCGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACgg              <  1:981545/139‑1 (MQ=255)                                                                                                          tttCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCgg        >  1:211053/1‑139 (MQ=255)                                                                                                           ttCTGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc       <  2:549331/139‑1 (MQ=255)                                                                                                           ttCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc       <  2:1037000/139‑1 (MQ=255)                                                                                                           ttCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc       <  2:1013387/139‑1 (MQ=255)                                                                                                                aTTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgcatgca  <  1:247271/139‑1 (MQ=255)                                                                                                                              ccTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTcgcg                                              >  2:102079/1‑81 (MQ=255)                                                                                                                              ccTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTcgcg                                              <  1:102079/81‑1 (MQ=255)                                                                                                                                 |                                                                                                                          TGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCA  >  NC_000913/3478674‑3478923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑