Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,501,096	T→C	L396P (CTC→CCC)	frlA →	putative fructoselysine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,501,096	0	T	C	100.0%	211.6 / NA	59	L396P (CTC→CCC)	frlA	putative fructoselysine transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/31);  total (28/31)

TGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTGGGCGAAGCGTAGCCGC  >  NC_000913/3500970‑3501226                                                                                                                               |                                                                                                                                   tgGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTc                                                                                                                        <  2:586932/139‑1 (MQ=255)     ttATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGc                                                                                                                    <  1:149082/139‑1 (MQ=255)     ttATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGc                                                                                                                    <  1:766605/139‑1 (MQ=255)             cccTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCt                                                                                                            >  1:1017120/1‑139 (MQ=255)             cccTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCt                                                                                                            >  1:1059397/1‑139 (MQ=255)              ccTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAATCCTCATTCTg                                                                                                           >  2:259642/1‑139 (MQ=255)               cTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTgg                                                                                                          <  1:1285327/139‑1 (MQ=255)                  gTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCg                                                                                                       >  2:1124894/1‑139 (MQ=255)                  gTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCg                                                                                                       >  2:411885/1‑139 (MQ=255)                     aTGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCt                                                                                                    <  1:1124894/139‑1 (MQ=255)                     aTGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCt                                                                                                    <  1:344907/139‑1 (MQ=255)                      tgtgTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTc                                                                                                   >  1:362486/1‑139 (MQ=255)                      tgtgTTTCAAAAATACCCTCACCTTCGGCTCCATCAGCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTc                                                                                                   <  1:814193/139‑1 (MQ=255)                        tgtTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGTTTTCGTAACCGCGACGATTACAAACCGCTGTGGCGTATTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTCCGCCAAGCCTCATTCTGGTCGCCTCaa                                                                                                 >  1:706735/1‑139 (MQ=255)                        tgtTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTc                                                                                                                        >  2:368601/1‑116 (MQ=255)                        tgtTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTc                                                                                                                        <  1:368601/116‑1 (MQ=255)                        tgtTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGcc                                                                                                                   <  1:988350/121‑1 (MQ=255)                        tgtTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGcc                                                                                                                   >  2:988350/1‑121 (MQ=255)                            tCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCtt                                                                                             >  2:253429/1‑139 (MQ=255)                            tCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCtt                                                                                             >  1:1133152/1‑139 (MQ=255)                                  aTACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCAt                                                                                                                              >  1:125904/1‑100 (MQ=255)                                  aTACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCAt                                                                                                                              <  2:125904/100‑1 (MQ=255)                                    acccggcccTTAGGCTCCTGCATCTGGTGTCGCAAACGCGACGAGTACAACCTGCTGTGGCTTCCTCCTGTTTTCAGGCTGAGGACCACCCCCTCCCTGGCGTCAAGCCTCATTATGGCCGCCTCAACCTTTGTCTggg                                                                                     <  2:288999/132‑1 (MQ=255)                                    aCCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCt                                                                                                    <  1:10204/124‑1 (MQ=255)                                    aCCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCt                                                                                                    >  2:10204/1‑124 (MQ=255)                                    aCCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTggg                                                                                     >  2:532469/1‑139 (MQ=255)                                       cTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCAc                                                                                  <  2:858982/139‑1 (MQ=255)                                        tCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCAcc                                                                                 <  1:532469/139‑1 (MQ=255)                                             ttCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTc                                                                            <  2:1133152/139‑1 (MQ=255)                                             ttCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTc                                                                            <  1:490561/139‑1 (MQ=255)                                                      atcatcTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGcc                                                                                                                   >  1:732711/1‑91 (MQ=255)                                                      atcatcTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGcc                                                                                                                   <  2:732711/91‑1 (MQ=255)                                                               tgtCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTc                                                                                                                        >  1:15022/1‑77 (MQ=255)                                                               tgtCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTc                                                                                                                        <  2:15022/77‑1 (MQ=255)                                                                   gTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCAt                                                      >  2:848576/1‑139 (MQ=255)                                                                    tAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATc                                                     >  2:1106875/1‑139 (MQ=255)                                                                     aaaCGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCg                                                    <  1:1106875/139‑1 (MQ=255)                                                                         gcgACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTAt                                                <  2:1017120/139‑1 (MQ=255)                                                                            acgaTTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTgg                                                                                                          >  2:970404/1‑78 (MQ=255)                                                                            acgaTTACAAACCGCTGTGGCGTACTCCGGCTTGCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTgg                                                                                                          <  1:970404/78‑1 (MQ=255)                                                                                    aaaCCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTc                                     <  1:411885/139‑1 (MQ=255)                                                                                       ccGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTctgc                                  <  1:1264563/139‑1 (MQ=255)                                                                                          cTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTc                                                                            >  1:924331/1‑94 (MQ=255)                                                                                          cTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTc                                                                            <  2:924331/94‑1 (MQ=255)                                                                                           tgtgGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTgg                                                                                                          >  2:240077/1‑63 (MQ=255)                                                                                           tgtgGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTgg                                                                                                          <  1:240077/63‑1 (MQ=255)                                                                                           tgtgGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGcc                                                           <  2:724464/110‑1 (MQ=255)                                                                                           tgtgGCGTACTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGcc                                                           >  1:724464/1‑110 (MQ=255)                                                                                                    cTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCAt                                                      >  1:1289299/1‑106 (MQ=255)                                                                                                    cTCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCAt                                                      <  2:1289299/106‑1 (MQ=255)                                                                                                     tCCGGCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCCACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTc                    >  1:1295091/1‑139 (MQ=255)                                                                                                         gCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGt                                      <  1:1285118/117‑1 (MQ=255)                                                                                                         gCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGt                                      <  2:679255/117‑1 (MQ=255)                                                                                                         gCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGt                                      >  2:1285118/1‑117 (MQ=255)                                                                                                         gCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGt                                      >  1:679255/1‑117 (MQ=255)                                                                                                         gCTTTCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTggg                >  1:43166/1‑139 (MQ=255)                                                                                                             tCGGGCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTGGGCGaa            <  1:848576/139‑1 (MQ=255)                                                                                                               gggCTGATGACCACCCCCGCCATTGCGTCAAGCCTCATTCTAGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTGGGCGAAGc          >  2:98320/1‑139 (MQ=255)                                                                                                                       gaCCACCCCCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTGGGCGAAGCGTAgccgc  <  2:1117776/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                   TGGGTTATTTCACCCTGGTGATGTGTTTCAAAAATACCCTCACCTTCGGCTCCATCATCTGGTGTCGTAAACGCGACGATTACAAACCGCTGTGGCGTACTCCGGCTTTCGGGCTGATGACCACCCTCGCCATTGCGTCAAGCCTCATTCTGGTCGCCTCAACCTTTGTCTGGGCACCGATTCCCGGCCTTATCTGCGCCGTCATCGTTATTGCTACTGGTCTGCCTGCTTACGCCTTCTGGGCGAAGCGTAGCCGC  >  NC_000913/3500970‑3501226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑