Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,820	A→G	intergenic (‑52/+14)	pyrE ← / ← rph	orotate phosphoribosyltransferase/ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,820	0	A	G	100.0%	201.6 / NA	56	intergenic (‑52/+14)	pyrE/rph	orotate phosphoribosyltransferase/ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/26);  total (30/26)

GCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTC  >  NC_000913/3815684‑3815944                                                                                                                                         |                                                                                                                             gcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGt                                                                                                                            <  1:695717/139‑1 (MQ=255)             gCGTAAACTAGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTTATCGGTCGCCTTAAAAAt                                                                                                                >  1:870462/1‑139 (MQ=255)                 aaaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTc                                                                                                                           <  2:404928/124‑1 (MQ=255)                 aaaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTc                                                                                                                           >  1:404928/1‑124 (MQ=255)                  aaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGtt                                                                                                           >  1:1177807/1‑139 (MQ=255)                            aCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGcgcc                                                                                                 <  2:667886/139‑1 (MQ=255)                                        gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTc                                                                                     >  2:560832/1‑139 (MQ=255)                                        gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTc                                                                                     >  1:341131/1‑139 (MQ=255)                                        gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTc                                                                                     >  1:1172168/1‑139 (MQ=255)                                        gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTc                                                                                     >  2:1293129/1‑139 (MQ=255)                                          ttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCgt                                                                                   <  2:359156/139‑2 (MQ=255)                                                  cgcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGt                                                                                                                            >  2:100958/1‑90 (MQ=255)                                                          cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgattcgatt                                                                   <  2:168030/139‑1 (MQ=255)                                                          cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgattcgatt                                                                   <  2:870462/139‑1 (MQ=255)                                                                     cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGc                                                                                                        <  1:1218089/91‑1 (MQ=255)                                                                     cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGc                                                                                                        >  2:1218089/1‑91 (MQ=255)                                                                     cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGc                                                                                        <  2:5479/107‑1 (MQ=255)                                                                     cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGc                                                                                        >  1:5479/1‑107 (MQ=255)                                                                          atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGc                                                   >  1:139532/1‑139 (MQ=255)                                                                            atGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCca                                                 <  1:560832/139‑1 (MQ=255)                                                                                    aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGc                                                         >  1:207279/1‑123 (MQ=255)                                                                                    aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGc                                                         <  2:207279/123‑1 (MQ=255)                                                                                         ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                    <  2:1172168/139‑1 (MQ=255)                                                                                         ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                    <  2:766824/139‑1 (MQ=255)                                                                                          tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGc                                                                                        >  1:610194/1‑86 (MQ=255)                                                                                          tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGc                                                                                        <  2:610194/86‑1 (MQ=255)                                                                                          tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                      <  1:83543/88‑1 (MQ=255)                                                                                          tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                      >  2:83543/1‑88 (MQ=255)                                                                                           cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCgt                                                                                   <  2:410022/90‑2 (MQ=255)                                                                                           cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCgt                                                                                   >  1:410022/1‑89 (MQ=255)                                                                                            gCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGCCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGctc                                 >  2:1050368/1‑139 (MQ=255)                                                                                                ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCa                             >  2:948121/1‑139 (MQ=255)                                                                                                          ttttCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACg                   >  2:619102/1‑139 (MQ=255)                                                                                                            ttCTACAGACAAAGAAAATGCGTCCCATCGGTCGGCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCCCGGGCCAGAGCCAACAAGATGAGTAGCTCTGCATGGGTGAACGGc                 <  2:1145957/139‑1 (MQ=255)                                                                                                             tCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgattcgatt                                                                   <  2:323797/88‑1 (MQ=255)                                                                                                             tCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgattcgatt                                                                   >  1:323797/1‑88 (MQ=255)                                                                                                              cTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACa                                                                               <  1:534776/75‑1 (MQ=255)                                                                                                              cTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACa                                                                               >  2:534776/1‑75 (MQ=255)                                                                                                               tACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTg                                                                                         <  2:706535/64‑1 (MQ=255)                                                                                                               tACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTg                                                                                         >  1:706535/1‑64 (MQ=255)                                                                                                               tACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCg                                                                                    >  2:421042/1‑69 (MQ=255)                                                                                                               tACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCg                                                                                    <  1:421042/69‑1 (MQ=255)                                                                                                                 cAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc            <  1:1246071/139‑1 (MQ=255)                                                                                                                    aCAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGcgcc                                                                                                 >  2:873616/1‑51 (MQ=255)                                                                                                                    aCAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGcgcc                                                                                                 <  1:873616/51‑1 (MQ=255)                                                                                                                    aCAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTc         >  1:102848/1‑139 (MQ=255)                                                                                                                    aCAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTc         >  2:1191104/1‑139 (MQ=255)                                                                                                                              ggCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                      >  2:455440/1‑52 (MQ=255)                                                                                                                              ggCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                      <  1:455440/52‑1 (MQ=255)                                                                                                                              ggCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc  <  1:1053243/136‑1 (MQ=255)                                                                                                                              ggCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc  >  2:1053243/1‑136 (MQ=255)                                                                                                                                cGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt                                                              >  2:1048590/1‑74 (MQ=255)                                                                                                                                cGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt                                                              <  1:1048590/74‑1 (MQ=255)                                                                                                                                cGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCca                                                 >  2:484220/1‑87 (MQ=255)                                                                                                                                cGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCca                                                 <  1:484220/87‑1 (MQ=255)                                                                                                                                  aCTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                               <  1:1106083/103‑1 (MQ=255)                                                                                                                                  aCTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                               >  2:1106083/1‑103 (MQ=255)                                                                                                                                      atcgGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                      <  1:256207/44‑1 (MQ=255)                                                                                                                                      atcgGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                      >  2:256207/1‑44 (MQ=255)                                                                                                                                         |                                                                                                                             GCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTC  >  NC_000913/3815684‑3815944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑