Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	335,970	C→T	Q16* (CAA→TAA)	yahE →	DUF2877 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	335,970	0	C	T	100.0%	168.6 / NA	53	Q16* (CAA→TAA)	yahE	DUF2877 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/26);  total (27/26)

GGAACGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACT  >  NC_000913/335834‑336095                                                                                                                                         |                                                                                                                              ggAACGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTaa                                                                                                                             >  1:577009/1‑139 (MQ=255)     cGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGAc                                                                                                                         <  1:898886/139‑1 (MQ=255)              gAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAAc                                                                                                                <  2:1285561/139‑1 (MQ=255)                     ttGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCtt                                                                                                         >  1:1291996/1‑139 (MQ=255)                       gcgcAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTg                                                                                                       >  1:287251/1‑139 (MQ=255)                       gcgcAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTg                                                                                                       >  1:789350/1‑139 (MQ=255)                        cgcAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGc                                                                                                      >  2:209111/1‑139 (MQ=255)                            gTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCaga                                                                                                  <  1:209111/139‑1 (MQ=255)                                cTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCgg                                                                                              >  2:851401/1‑139 (MQ=255)                                      gCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGAGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAata                                                                                        >  2:926586/1‑139 (MQ=255)                                       ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAatat                                                                                       >  1:737573/1‑139 (MQ=255)                                                 aTAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCg                                                                             >  2:1258327/1‑139 (MQ=255)                                                  tAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCgg                                                                            >  1:1034472/1‑139 (MQ=255)                                                       cGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGTGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGc                                                                       >  1:387783/1‑139 (MQ=255)                                                         ggAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGACGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATAGCCCTCCCGGCTCGCca                                                                     >  1:795637/1‑139 (MQ=255)                                                          gAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCcag                                                                    <  2:737573/139‑1 (MQ=255)                                                               ttGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGtt                                                               >  1:793779/1‑139 (MQ=255)                                                                      cAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACg                                                        <  1:851401/139‑1 (MQ=255)                                                                      cAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACg                                                        <  2:1291996/139‑1 (MQ=255)                                                                        acacacCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgct                                                      <  1:1260816/139‑1 (MQ=255)                                                                           cacCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgctgct                                                   <  1:926586/139‑1 (MQ=255)                                                                            acCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCtt                                                  >  2:1268584/1‑139 (MQ=255)                                                                            acCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCtt                                                  >  2:71771/1‑139 (MQ=255)                                                                            acCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCACCAGCAGTTGCTGACGCTGCtt                                                  >  2:665018/1‑139 (MQ=255)                                                                               aGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgt                                               >  1:992727/1‑139 (MQ=255)                                                                                gAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                              <  2:481476/139‑1 (MQ=255)                                                                                gAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                              <  2:577009/139‑1 (MQ=255)                                                                                gAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                              <  2:789350/139‑1 (MQ=255)                                                                                gAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                              <  2:793779/139‑1 (MQ=255)                                                                                gAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                              <  2:1034472/139‑1 (MQ=255)                                                                                gAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                              <  2:124003/139‑1 (MQ=255)                                                                                  aaGACGGTGTGTGTGGGCGCTAACTGCGGAGGCGGATTTTCTGGCGCAGCGGGGGTAAGGACGGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGATGACGCTGCTTTGTGaa                                            <  2:281884/139‑1 (MQ=255)                                                                                      cGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgct                                                      >  1:551264/1‑125 (MQ=255)                                                                                      cGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgct                                                      <  2:551264/125‑1 (MQ=255)                                                                                        gtgtgtgtGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCg                                                                                               >  2:1279364/1‑82 (MQ=255)                                                                                        gtgtgtgtGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCg                                                                                               <  1:1279364/82‑1 (MQ=255)                                                                                          gtgtgtgGGCGCTAACTGCGGATGCGGGTTTTCTGGCGCAGCGGGGGTAGGGACAGGTTGAACAGGGCGTTGCCAGAGCGGTAAATATCGCACTCCCGGCTAGCCGGCAGTTGCTGGCGCTGCTTTGTGAAGAGTACGa                                    >  2:1282856/1‑139 (MQ=255)                                                                                           tgtgtgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCgg                                                                            <  1:903916/98‑1 (MQ=255)                                                                                           tgtgtgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCgg                                                                            >  2:903916/1‑98 (MQ=255)                                                                                               tgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                           <  1:826987/47‑1 (MQ=255)                                                                                               tgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                           >  2:826987/1‑47 (MQ=255)                                                                                                   cgcTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGcc                           >  2:288376/1‑139 (MQ=255)                                                                                                     cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCaa                         >  2:651110/1‑139 (MQ=255)                                                                                                            cggatgcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTg                  <  1:1258327/139‑1 (MQ=255)                                                                                                                tgcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                              <  2:166401/107‑1 (MQ=255)                                                                                                                tgcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTtgtg                                              >  1:166401/1‑107 (MQ=255)                                                                                                                  cggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgctgct                                                   >  2:345118/1‑100 (MQ=255)                                                                                                                  cggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgctgct                                                   <  1:345118/100‑1 (MQ=255)                                                                                                                          cTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactca    <  1:651110/139‑1 (MQ=255)                                                                                                                            ggCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcact  <  1:193429/139‑1 (MQ=255)                                                                                                                            ggCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcact  <  1:1268584/139‑1 (MQ=255)                                                                                                                                   cGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgctgct                                                   >  2:382890/1‑83 (MQ=255)                                                                                                                                   cGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgctgct                                                   <  1:382890/83‑1 (MQ=255)                                                                                                                                         |                                                                                                                              GGAACGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACT  >  NC_000913/335834‑336095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑