Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,862,497 C→T pseudogene (1107/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,862,4970CT100.0% 237.6 / NA 73pseudogene (1107/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/40);  total (33/40)

GCACATGTTGAACTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTT  >  NC_000913/3862362‑3862630
                                                                                                                                       |                                                                                                                                     
gCACATGTTGAACTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAg                                                                                                                                    <  2:675016/139‑1 (MQ=255)
  acaTGTTGAACTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTc                                                                                                                                  <  1:305359/139‑1 (MQ=255)
      gTTGAACTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATc                                                                                                                              >  2:1177298/1‑139 (MQ=255)
           aCTGAAGAATCAGTGTGCGGAAAACCACGAAGTACAGAAGGGTAGAGCGCAACCCAATAGCGATTTGGGGCAGCATCATATCCGCGTGGTTGCTGAACATCGAGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGAcc                                                                                                                         >  2:1272636/1‑139 (MQ=255)
              gCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTcc                                                                                                                      >  1:475788/1‑139 (MQ=255)
               cAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                     <  1:889987/139‑1 (MQ=255)
               cAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                     <  1:333395/139‑1 (MQ=255)
               cAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                     >  2:129304/1‑139 (MQ=255)
                 gAATGAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCAGCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATc                                                                                                                              >  2:751498/1‑128 (MQ=255)
                 gAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATc                                                                                                                              <  1:751498/128‑1 (MQ=255)
                 gAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                   <  2:475788/139‑1 (MQ=255)
                  aaTCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                     >  2:873264/1‑136 (MQ=255)
                  aaTCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                     <  1:873264/136‑1 (MQ=255)
                      aGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGtt                                                                                                             <  1:129304/139‑1 (MQ=255)
                        tgggcggAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTca                                                                                                           <  2:1156808‑M1/136‑3 (MQ=255)
                         gtgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                   <  2:167281/131‑1 (MQ=255)
                         gtgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                   >  1:167281/1‑131 (MQ=255)
                         gtgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcac                                                                                                          >  1:1184754‑M1/1‑136 (MQ=255)
                          tgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcacc                                                                                                         >  2:231101‑M1/1‑135 (MQ=255)
                          tgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcacc                                                                                                         <  1:494650‑M1/139‑5 (MQ=255)
                            cGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcac                                                                                                          >  2:570232‑M1/1‑133 (MQ=255)
                            cGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcac                                                                                                          <  1:570232‑M1/136‑4 (MQ=255)
                              gAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacac                                                                                                     <  1:109191‑M1/139‑9 (MQ=255)
                                  aCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGCTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaa                                                                                                 >  2:960520‑M1/1‑127 (MQ=255)
                                  aCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                   <  1:1238137/122‑1 (MQ=255)
                                  aCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                   >  2:1238137/1‑122 (MQ=255)
                                   ccACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaag                                                                                                <  1:555590‑M1/139‑14 (MQ=255)
                                   ccACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaag                                                                                                <  1:623684‑M1/139‑14 (MQ=255)
                                   ccACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaag                                                                                                <  2:302242‑M1/139‑14 (MQ=255)
                                    cACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAg                                                                                                                                    <  1:149518/103‑1 (MQ=255)
                                    cACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAg                                                                                                                                    >  2:149518/1‑103 (MQ=255)
                                            aCAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacatt                                                                                       <  1:793731‑M1/139‑23 (MQ=255)
                                                 agGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggt                                                                                  >  1:713406‑M1/1‑112 (MQ=255)
                                                 agGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggt                                                                                  <  2:1189546‑M1/139‑28 (MQ=255)
                                                   ggTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAg                                                                                                                                    <  1:546927/88‑1 (MQ=255)
                                                   ggTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAg                                                                                                                                    >  2:546927/1‑88 (MQ=255)
                                                     tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgac                                                                              >  2:51696‑M1/1‑108 (MQ=255)
                                                               cccAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGa                                                                                                                           >  2:29316/1‑85 (MQ=255)
                                                               cccAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGa                                                                                                                           <  1:29316/85‑1 (MQ=255)
                                                               cccAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccg                                                                    >  1:1016786‑M1/1‑98 (MQ=255)
                                                               cccAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccg                                                                    <  1:960520‑M1/139‑42 (MQ=255)
                                                                       cGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTccccacaccaa                                                                                                  >  2:330085‑M1/1‑90 (MQ=255)
                                                                       cGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaa                                                                                                  <  1:330085‑M1/101‑12 (MQ=255)
                                                                         aTTTGGGTCAGCATCATATCCGCGTGTTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacatt                                                                                       <  1:688433‑M1/110‑23 (MQ=255)
                                                                         aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattgcggtcgacattgaggccgccagcacggc                                                          <  2:535715‑M1/139‑52 (MQ=255)
                                                                         aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggc                                                          >  1:535715‑M1/1‑88 (MQ=255)
                                                                         aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacatt                                                                                       >  2:688433‑M1/1‑88 (MQ=255)
                                                                         aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacac                                                                                                     <  1:403357‑M1/96‑9 (MQ=255)
                                                                         aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacac                                                                                                     >  2:403357‑M1/1‑88 (MQ=255)
                                                                                 cAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccg                                                  >  2:416937‑M1/1‑80 (MQ=255)
                                                                                           tCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaa                                        <  1:51696‑M1/139‑70 (MQ=255)
                                                                                             cgcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacg                                      <  1:1177298‑M1/139‑72 (MQ=255)
                                                                                              gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcac                                                                                                          <  2:20571‑M1/70‑4 (MQ=255)
                                                                                              gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcac                                                                                                          >  1:20571‑M1/1‑67 (MQ=255)
                                                                                                  ggTTGCTGAACATCGGGATCCAGTTTTCCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaa                                 <  2:738432‑M1/139‑77 (MQ=255)
                                                                                                   gTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaa                                >  2:1023840‑M1/1‑62 (MQ=255)
                                                                                                   gTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggt                                                                                  >  2:468882‑M1/1‑62 (MQ=255)
                                                                                                   gTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggt                                                                                  <  1:468882‑M1/89‑28 (MQ=255)
                                                                                                       cTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaa                            <  2:1016786‑M1/139‑82 (MQ=255)
                                                                                                           aCATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacagg                        <  1:1023840‑M1/139‑86 (MQ=255)
                                                                                                              tCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaag                     >  1:63321‑M1/1‑51 (MQ=255)
                                                                                                               cGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagtgatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaagg                    >  2:846635‑M1/1‑50 (MQ=255)
                                                                                                               cGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaagg                    >  2:44919‑M1/1‑50 (MQ=255)
                                                                                                                 ggATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggta                  >  1:470481‑M1/1‑48 (MQ=255)
                                                                                                                 ggATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTT‑‑‑CACCACACCAAagag                                                                                           <  2:822739/63‑1 (MQ=255)
                                                                                                                 ggATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGT‑‑‑ACACCACACCAAagag                                                                                           >  1:822739/1‑63 (MQ=255)
                                                                                                                         ttttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccag          <  1:231101‑M1/139‑100 (MQ=255)
                                                                                                                            tGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccagcgg       <  2:63321‑M1/139‑103 (MQ=255)
                                                                                                                              cGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgt                                                      >  2:1097409‑M1/1‑35 (MQ=255)
                                                                                                                              cGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgt                                                      <  1:1097409‑M1/90‑56 (MQ=255)
                                                                                                                                 tAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccagcggttcgg  <  1:224524‑M1/139‑108 (MQ=255)
                                                                                                                                   aaaCTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagca                                         >  2:540932‑M1/1‑30 (MQ=255)
                                                                                                                                   aaaCTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagca                                         <  1:540932‑M1/98‑69 (MQ=255)
                                                                                                                                       |                                                                                                                                     
GCACATGTTGAACTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTT  >  NC_000913/3862362‑3862630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: