Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 340,089 G→A intergenic (+346/‑76) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913340,0890GA100.0% 142.4 / NA 45intergenic (+346/‑76)yahG/yahIDUF1116 family protein/carbamate kinase‑like protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/25);  total (20/25)

CTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATC  >  NC_000913/339963‑340209
                                                                                                                              |                                                                                                                        
cTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCg                                                                                                              <  1:141596/139‑1 (MQ=255)
            aCGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                  >  2:765201/1‑139 (MQ=255)
                   gcgcGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTg                                                                                           >  2:959543/1‑139 (MQ=255)
                                tAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATaa                                                                              <  2:535997/139‑1 (MQ=255)
                                    ccTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                  >  1:276869/1‑115 (MQ=255)
                                    ccTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                  <  2:276869/115‑1 (MQ=255)
                                     cTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCAt                                                                                                      >  1:380577/1‑110 (MQ=255)
                                     cTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCAt                                                                                                      <  2:380577/110‑1 (MQ=255)
                                        aTAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCa                                                                      <  2:452056/139‑1 (MQ=255)
                                        aTAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCa                                                                      >  1:452056/1‑139 (MQ=255)
                                            gCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCct                                                                  <  2:41572/139‑1 (MQ=255)
                                             cGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCctc                                                                 >  1:295961/1‑139 (MQ=255)
                                              gTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                        <  2:989703/115‑1 (MQ=255)
                                              gTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                        >  1:989703/1‑115 (MQ=255)
                                                           cATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                   <  2:620566/139‑1 (MQ=255)
                                                                    ccATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGaa                                          >  1:39106/1‑139 (MQ=255)
                                                                        tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGTGCATCAGGCAGTCTGGCGTTGGTCATAAcc                                                                            >  1:472034/1‑101 (MQ=255)
                                                                        tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGTGCATCAGGCAGTCTGGCGTTGGTCATAAcc                                                                            <  2:472034/101‑1 (MQ=255)
                                                                        tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                  <  2:1247314/79‑1 (MQ=255)
                                                                        tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACAt                                                     >  1:440638/1‑124 (MQ=255)
                                                                        tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACAt                                                     <  2:440638/124‑1 (MQ=255)
                                                                        tGCCGGATGCGGCGTGAACGCCTTATACGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                  >  1:1247314/1‑79 (MQ=14)
                                                                           cGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTa                                                                                                            >  2:701245/1‑66 (MQ=255)
                                                                           cGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTa                                                                                                            <  1:701245/66‑1 (MQ=255)
                                                                                gcggcgTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGt                              <  1:959543/139‑1 (MQ=255)
                                                                                    cgTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa                                                                         <  2:277449/92‑1 (MQ=255)
                                                                                    cgTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa                                                                         >  1:277449/1‑92 (MQ=255)
                                                                                        aaCGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                   <  1:41885/110‑1 (MQ=255)
                                                                                        aaCGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                   >  2:41885/1‑110 (MQ=255)
                                                                                            ccTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATtgg                  <  1:238548/139‑1 (MQ=255)
                                                                                             cTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                  >  1:5778/1‑58 (MQ=255)
                                                                                             cTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                  <  2:5778/58‑1 (MQ=255)
                                                                                                   cGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAAc           <  1:765201/139‑1 (MQ=255)
                                                                                                     gCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCct                                                                  <  1:661368/82‑1 (MQ=255)
                                                                                                     gCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCct                                                                  >  2:661368/1‑82 (MQ=255)
                                                                                                       cTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa       <  2:295961/139‑1 (MQ=255)
                                                                                                           gAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGaaa                                         >  1:340334/1‑101 (MQ=255)
                                                                                                           gAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGaaa                                         <  2:340334/101‑1 (MQ=255)
                                                                                                            aGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATc  <  2:39106/139‑1 (MQ=255)
                                                                                                                gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                  >  1:369529/1‑39 (MQ=255)
                                                                                                                gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAgg                                                                                                  <  2:369529/39‑1 (MQ=14)
                                                                                                                gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                        >  1:480475/1‑49 (MQ=255)
                                                                                                                gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                        <  2:480475/49‑1 (MQ=255)
                                                                                                                gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAg                                                            <  2:1102658/77‑1 (MQ=255)
                                                                                                                gcgcGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAg                                                            >  1:1102658/1‑77 (MQ=255)
                                                                                                                              |                                                                                                                        
CTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATC  >  NC_000913/339963‑340209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: