Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,024,336 G→A *261* (TAG→TAA
L162L (CTC→CTT
tatD →
rfaH ←
quality control of Tat‑exported FeS proteins, Mg‑dependent cytoplasmic DNase
transcription antitermination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,024,3360GA100.0% 334.0 / NA 103*261* (TAG→TAA
L162L (CTC→CTT
tatD
rfaH
quality control of Tat‑exported FeS proteins, Mg‑dependent cytoplasmic DNase
transcription antitermination protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (50/53);  total (50/53)

Only 100 of 103 total aligned reads displayed.

ATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGT  >  NC_000913/4024204‑4024467
                                                                                                                                    |                                                                                                                                   
atcCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTg                                                                                                                               <  2:1063294/139‑1 (MQ=255)
         cAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCg                                                                                                                      <  2:314498/139‑1 (MQ=255)
           aCGCGCTAGCTCATCTGCCGCAGATTCTGCAGCGTGTTGCGCTCTGGCGTGGAGAAGATGCGGCATGGGTGGATGGGACCGCGGAGGCTAATGTCAAATCAGTGTTTGGGATTGCGTTTTAAAGTTTGCGAAACTCggg                                                                                                                    <  2:729475/139‑2 (MQ=255)
           aCGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                    <  1:296849/139‑1 (MQ=255)
           aCGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                    <  1:966946/139‑1 (MQ=255)
             gAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAt                                                                                                                  <  2:1102194/139‑1 (MQ=255)
                ccagccCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAttct                                                                                                               <  2:184392/139‑1 (MQ=255)
                     ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                          <  1:18052/139‑1 (MQ=255)
                      cATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                         <  2:1092948/139‑1 (MQ=255)
                       aTCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACt                                                                                                        <  1:1079608/139‑1 (MQ=255)
                         cTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACtgt                                                                                                      >  1:380641/1‑139 (MQ=255)
                         cTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACtgt                                                                                                      >  2:469745/1‑139 (MQ=255)
                          tGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                    <  1:912497/124‑1 (MQ=255)
                            ccccATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                    <  1:747420/122‑1 (MQ=255)
                            ccccATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                    >  2:747420/1‑122 (MQ=255)
                             cccATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                          >  2:586868/1‑131 (MQ=255)
                             cccATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                          <  1:586868/131‑1 (MQ=255)
                             cccATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCtt                                                                                                  >  1:1285807/1‑139 (MQ=255)
                                    tttGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATctct                                                                                           >  2:1286806/1‑139 (MQ=255)
                                      tGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCg                                                                                                                             <  1:200298/103‑1 (MQ=255)
                                      tGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCttt                                                                                         >  1:444435/1‑139 (MQ=255)
                                      tGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTGTTAAAGTTTGCg                                                                                                                             >  2:200298/1‑103 (MQ=255)
                                           cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGCTTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                    >  2:791695/1‑139 (MQ=255)
                                           cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                    <  1:1257212/107‑1 (MQ=255)
                                           cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                    >  2:1257212/1‑107 (MQ=255)
                                           cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTca                                                                                                            <  2:328956/115‑1 (MQ=255)
                                           cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTca                                                                                                            >  1:328956/1‑115 (MQ=255)
                                           cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                    >  1:1051880/1‑139 (MQ=255)
                                            gTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaat                                                                                   <  1:469745/139‑1 (MQ=255)
                                                tGCGCACTGGCGTGTAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAAAACTGTTTGGGATTGCGTTTTAAAGATTGCGGAACTCGGTATTCTTCACACTATGCTTAATCTCTTTATGAATAAGa                                                                               <  1:791695/139‑1 (MQ=255)
                                                  cgcACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                         <  2:552980/111‑1 (MQ=255)
                                                  cgcACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                         >  1:552980/1‑111 (MQ=255)
                                                  cgcACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGAtt                                                                             <  1:59022/139‑1 (MQ=255)
                                                   gcACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTa                                                                            <  2:380641/139‑1 (MQ=255)
                                                   gcACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTa                                                                            <  2:106882/139‑1 (MQ=255)
                                                     aCTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                         <  1:407676/108‑1 (MQ=255)
                                                     aCTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                         >  2:407676/1‑108 (MQ=255)
                                                     aCTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAg                                                                          >  1:242168/1‑139 (MQ=255)
                                                       tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                         >  2:590965/1‑106 (MQ=255)
                                                       tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                         <  1:590965/106‑1 (MQ=255)
                                                         gCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                    <  1:1113732/93‑1 (MQ=255)
                                                         gCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                    >  2:1113732/1‑93 (MQ=255)
                                                         gCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                    <  1:1043189/125‑1 (MQ=255)
                                                         gCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                    >  2:1043189/1‑125 (MQ=255)
                                                         gCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAAt                                                                      >  1:1042883/1‑139 (MQ=255)
                                                            tGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAAtc                                                                                              >  2:881704/1‑112 (MQ=255)
                                                            tGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAAtc                                                                                              <  1:881704/112‑1 (MQ=255)
                                                                   gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgagc                                                            >  2:392889/1‑139 (MQ=255)
                                                                         gCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAGGCAATAGCATGGAGCGAGCCt                                                      >  1:1069331/1‑139 (MQ=255)
                                                                         gCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgag                                                             <  1:1264839/132‑1 (MQ=255)
                                                                         gCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgag                                                             >  2:1264839/1‑132 (MQ=255)
                                                                         gCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCt                                                      <  2:242168/139‑1 (MQ=255)
                                                                           aTGGCTGGCTACCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCa                                                    >  1:204308/1‑139 (MQ=255)
                                                                                  gctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGAtt                                                                             >  1:197664/1‑107 (MQ=255)
                                                                                  gctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGAtt                                                                             <  2:197664/107‑1 (MQ=255)
                                                                                    tgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTa                                                                                                                   <  1:448237/67‑1 (MQ=255)
                                                                                    tgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTa                                                                                                                   >  2:448237/1‑67 (MQ=255)
                                                                                       caccacGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAttctt                                                                                                              <  1:300944/69‑1 (MQ=255)
                                                                                       caccacGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAttctt                                                                                                              >  2:300944/1‑69 (MQ=255)
                                                                                                 gCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGt                                           >  1:401200/1‑126 (MQ=255)
                                                                                                 gCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGt                                           <  2:401200/126‑1 (MQ=255)
                                                                                                    aaTGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACt                                                                                                                        <  1:528941/46‑1 (MQ=255)
                                                                                                    aaTGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACt                                                                                                                        >  2:528941/1‑46 (MQ=255)
                                                                                                    aaTGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCt                           >  1:426515/1‑139 (MQ=255)
                                                                                                      tGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGa                         <  2:204308/139‑1 (MQ=255)
                                                                                                      tGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGa                         <  2:444435/139‑1 (MQ=255)
                                                                                                        tCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                    <  1:1242873/46‑1 (MQ=255)
                                                                                                          aaaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAt                                                                                                                  <  2:1140167/46‑1 (MQ=255)
                                                                                                          aaaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAt                                                                                                                  >  1:1140167/1‑46 (MQ=255)
                                                                                                          aaaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                          <  2:1027418/54‑1 (MQ=255)
                                                                                                          aaaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                          >  1:1027418/1‑54 (MQ=255)
                                                                                                           aaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGcc                    >  1:1279023/1‑139 (MQ=255)
                                                                                                               acTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGa                                                                               >  2:1009734/1‑76 (MQ=255)
                                                                                                               acTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGa                                                                               <  1:1009734/76‑1 (MQ=255)
                                                                                                                  gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACATtgt                                                                                                      <  2:834313/50‑1 (MQ=255)
                                                                                                                  gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACtgt                                                                                                      >  1:834313/1‑50 (MQ=255)
                                                                                                                  gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTa                                                                            >  2:1046550/1‑76 (MQ=255)
                                                                                                                  gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTa                                                                            <  1:1046550/76‑1 (MQ=255)
                                                                                                                  gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGaaaa                                 >  2:277955/1‑119 (MQ=255)
                                                                                                                  gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGaaaa                                 <  1:277955/119‑1 (MQ=255)
                                                                                                                           tGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                          <  1:1206667/37‑1 (MQ=255)
                                                                                                                           tGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                          >  2:1206667/1‑37 (MQ=255)
                                                                                                                           tGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                         >  1:1062403/1‑38 (MQ=255)
                                                                                                                           tGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                         <  2:1062403/38‑1 (MQ=255)
                                                                                                                           tGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCa                                                                  <  1:467158/77‑1 (MQ=255)
                                                                                                                           tGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCa                                                                  >  2:467158/1‑77 (MQ=255)
                                                                                                                             tgTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                         >  2:1275405/2‑36 (MQ=255)
                                                                                                                             tgTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                         <  1:1275405/35‑1 (MQ=255)
                                                                                                                             cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAAt                                                                                               >  2:1187127/1‑46 (MQ=255)
                                                                                                                             cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAAt                                                                                               <  1:1187127/46‑1 (MQ=255)
                                                                                                                             cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                    >  1:872556/1‑57 (MQ=255)
                                                                                                                             cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                    <  2:872556/57‑1 (MQ=255)
                                                                                                                             cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTa                                                                            <  2:1213508/65‑1 (MQ=255)
                                                                                                                             cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTa                                                                            >  1:1213508/1‑65 (MQ=255)
                                                                                                                             cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCa                                                                        >  2:195194/1‑69 (MQ=255)
                                                                                                                             cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCa                                                                        <  1:195194/69‑1 (MQ=255)
                                                                                                                             cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGt  >  1:1276256/1‑139 (MQ=255)
                                                                                                                             cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGt  <  2:1285807/139‑1 (MQ=255)
                                                                                                                             cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGt  >  1:661673/1‑139 (MQ=255)
                                                                                                                             cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGt  >  1:853880/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                   
ATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGT  >  NC_000913/4024204‑4024467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: