| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,061,157 | G→A | *237* (TAG→TAA) | yihL → | putative DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,061,157 | 0 | G | A | 97.4% | 233.2 / ‑2.0 | 77 | *237* (TAG→TAA) | yihL | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (38/37); total (39/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.12e-01 | |||||||||||
AGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAT > NC_000913/4061024‑4061281 | aGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGtt < 2:703426/139‑1 (MQ=255) cTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATg > 2:1194944/1‑139 (MQ=255) ttGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATgg < 1:1024438/139‑1 (MQ=255) tGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGt > 1:635261/1‑139 (MQ=255) tGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGt < 2:725160/139‑1 (MQ=255) gTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACaa > 2:580717/1‑139 (MQ=255) tCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACaata > 2:221536/1‑139 (MQ=255) tGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACaataaa > 2:1171441/1‑139 (MQ=255) cGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACaataaata > 1:1139250/1‑139 (MQ=255) cGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACaataaata < 1:580717/139‑1 (MQ=255) gCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaag < 1:1158379/139‑1 (MQ=255) gATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGAt > 2:136669/1‑139 (MQ=255) tGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACg < 1:1171441/139‑1 (MQ=255) gCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGa < 2:28995/139‑1 (MQ=255) gCATCGCGCCGACCAGCAAGGCAATAGTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGa < 1:968566/139‑1 (MQ=255) cATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa < 2:813112/119‑1 (MQ=255) cATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa > 1:813112/1‑119 (MQ=255) cATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTAc < 2:259374/128‑1 (MQ=255) cATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTAc > 1:259374/1‑128 (MQ=255) cATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGac > 2:1148412/1‑139 (MQ=255) cATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGac > 1:562286/1‑139 (MQ=255) cAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtt < 2:1139250/139‑1 (MQ=255) atatTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATg < 1:221536/139‑1 (MQ=255) tatTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa < 1:519198/96‑1 (MQ=255) tatTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa > 2:519198/1‑96 (MQ=255) tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGacac < 2:1091787/113‑1 (MQ=255) tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGacac > 1:1091787/1‑113 (MQ=255) tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGaca > 1:259776/1‑112 (MQ=255) tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGaca < 2:259776/112‑1 (MQ=255) aaaTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCt > 1:1184600/1‑139 (MQ=255) aTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATgg < 2:194283/65‑1 (MQ=255) aTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATgg > 1:194283/1‑65 (MQ=255) aTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCct < 1:876098/115‑1 (MQ=255) aTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCct > 2:876098/1‑115 (MQ=255) aTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCttt > 1:414663/1‑139 (MQ=255) gATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCAt < 2:1053361/139‑1 (MQ=255) tatTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa < 1:1048876/81‑1 (MQ=255) tatTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa > 2:1048876/1‑81 (MQ=255) tatTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCt > 2:292824/1‑132 (MQ=255) tatTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCt < 1:292824/132‑1 (MQ=255) tGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTaaa > 2:708526/1‑139 (MQ=255) tatTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATg < 1:136669/139‑1 (MQ=255) atTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACa < 1:7715/83‑1 (MQ=255) atTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACa > 2:7715/1‑83 (MQ=255) ttGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCg > 1:335506/1‑139 (MQ=255) tGGCGATTTGAGGCCGTAGACCTCATCGTTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaaa > 2:268360/1‑72 (MQ=255) tGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaaa < 1:268360/72‑1 (MQ=255) tGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACaa < 2:995675/82‑1 (MQ=255) tGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACaa < 2:72979/82‑1 (MQ=255) tGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACaa > 1:995675/1‑82 (MQ=255) tGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACaa > 1:72979/1‑82 (MQ=255) tGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATg > 1:509451/1‑136 (MQ=255) tGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATg < 2:509451/136‑1 (MQ=255) gCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCt < 2:635261/139‑1 (MQ=255) ttGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAataaataa > 1:1030032/1‑56 (MQ=255) ttGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAataaataa < 2:1030032/56‑1 (MQ=255) tAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTAc > 2:163804/1‑94 (MQ=255) tAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTAc < 1:163804/94‑1 (MQ=255) tAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAata < 1:820449/118‑1 (MQ=255) tAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAata > 2:820449/1‑118 (MQ=255) tAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGc > 2:1292282/1‑139 (MQ=255) tAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCtt > 1:839939/1‑107 (MQ=255) tAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTACTCTTTATTTACATGCTTATGCCtt < 2:839939/107‑1 (MQ=255) gACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCct < 1:1148412/139‑1 (MQ=255) aCCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCctg < 1:1292282/139‑1 (MQ=255) catcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGaca < 1:171567/69‑1 (MQ=255) catcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGaca > 2:171567/1‑69 (MQ=255) catcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCtt < 2:725962/100‑1 (MQ=255) catcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCtt > 1:725962/1‑100 (MQ=255) catcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGa > 1:519305/1‑139 (MQ=255) atcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGacac > 2:1126462/1‑69 (MQ=255) atcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGacac < 1:1126462/69‑1 (MQ=255) atcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAt > 2:1273343/1‑139 (MQ=255) ctCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTAc > 1:694608/2‑79 (MQ=255) aTCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTAc < 2:694608/79‑1 (MQ=255) tGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCctgc < 2:171762/125‑1 (MQ=255) tGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCctgc > 1:171762/1‑125 (MQ=255) | AGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAT > NC_000913/4061024‑4061281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |