Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,325,152	A→G	I5T (ATC→ACC)	phnC ←	phosphonate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,325,152	0	A	G	100.0%	174.8 / NA	50	I5T (ATC→ACC)	phnC	phosphonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/27);  total (23/27)

TGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGATAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACGGGAATTCGATGACAGTGCGGGA  >  NC_000913/4325020‑4325278                                                                                                                                     |                                                                                                                               tGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCg                                                                                                                          >  1:994678/1‑139 (MQ=255)               ggATATTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAg                                                                                                            <  2:309772/139‑1 (MQ=255)                gATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGc                                                                                                           <  1:274846/139‑1 (MQ=255)                                          cAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGGATGCAGCGCTTGATGCTGATTGATGGTTTTGGCGAGCTTCTCGACACGGGTAATCGGTTGCATGATGCAGCCTCCCGAAAAAAGTGGCTCCATCGTg                                                                                 <  2:706218/139‑1 (MQ=255)                                          cAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTg                                                                                 <  1:47843/139‑1 (MQ=255)                                             agCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCat                                                                                                                   <  1:1268798/102‑1 (MQ=255)                                             agCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCat                                                                                                                   >  2:1268798/1‑102 (MQ=255)                                                  ccaTTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGa                                                                            <  2:73343/136‑1 (MQ=255)                                                  ccaTTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGa                                                                            >  1:73343/1‑136 (MQ=255)                                                   caTTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGc                                                                                                           >  1:913553/1‑104 (MQ=255)                                                   caTTTCACCGTGATGAATGTTCAGAGCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGc                                                                                                           <  2:913553/104‑1 (MQ=255)                                                      ttCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATtgt                                                                     <  2:994678/139‑1 (MQ=255)                                                                       tCAGATCAACCGCATGCAGCGCCTGATGCTGTTTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCat                                                                                                                   <  2:915828/76‑1 (MQ=255)                                                                       tCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCat                                                                                                                   >  1:915828/1‑76 (MQ=255)                                                                        cAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGttaattaa                                                   <  2:657843/139‑1 (MQ=255)                                                                         aGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTgg                                                           >  1:89252/1‑130 (MQ=255)                                                                         aGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTgg                                                           <  2:89252/130‑1 (MQ=255)                                                                               aCCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGc                                                                                                                     >  2:524133/1‑66 (MQ=255)                                                                               aCCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGc                                                                                                                     <  1:524133/66‑1 (MQ=255)                                                                               aCCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGAc                                            >  1:494042/1‑139 (MQ=255)                                                                                ccGGATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGa                                                                            <  1:815996/106‑1 (MQ=255)                                                                                ccGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTa                                                                                                                              >  1:21744/1‑56 (MQ=255)                                                                                ccGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTa                                                                                                                              <  2:21744/56‑1 (MQ=255)                                                                                ccGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGc                                                                                          >  1:13522/1‑92 (MQ=255)                                                                                ccGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGc                                                                                          <  2:13522/92‑1 (MQ=255)                                                                                ccGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCTTGGCGGa                                                                            >  2:815996/1‑106 (MQ=255)                                                                                  gCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTaa                                         <  2:494042/139‑1 (MQ=255)                                                                                     tGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAg                                      >  2:421221/1‑139 (MQ=255)                                                                                          cgcCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGaa                                 <  1:421221/139‑1 (MQ=255)                                                                                            ccTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCa                                                                                                                    <  1:143470/54‑1 (MQ=255)                                                                                            ccTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCa                                                                                                                    >  2:143470/1‑54 (MQ=255)                                                                                                aTGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTa                           >  1:951112/1‑139 (MQ=255)                                                                                                aTGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTa                           >  1:742517/1‑139 (MQ=255)                                                                                                  gCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCa                                                                                                                    >  1:384611/1‑48 (MQ=255)                                                                                                  gCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCa                                                                                                                    <  2:384611/48‑1 (MQ=255)                                                                                                  gCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGc                                                                                                           >  1:288181/1‑57 (MQ=255)                                                                                                  gCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGc                                                                                                           <  2:288181/57‑1 (MQ=255)                                                                                                  gCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAAc                         <  2:1122118/139‑1 (MQ=255)                                                                                                    tGATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGACGGATTATTGTGACGAGTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACgg                       >  1:106638/1‑139 (MQ=255)                                                                                                     gATTGAAGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACggg                      <  1:1135986/139‑1 (MQ=255)                                                                                                           aGGTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACGGGAATTCg                >  2:1247731/1‑139 (MQ=255)                                                                                                            ggTTTTGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACGGGAATTCGa               <  2:742517/139‑1 (MQ=255)                                                                                                               tttGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACGGGAATTCGATGa            <  2:1158008/139‑1 (MQ=255)                                                                                                                ttGGCGAGCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACGGGAATTCGATGAc           >  2:205108/1‑139 (MQ=255)                                                                                                                       gCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACggg                      <  2:543279/121‑1 (MQ=255)                                                                                                                       gCTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACggg                      >  1:543279/1‑121 (MQ=255)                                                                                                                        cTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTc                                                                                        <  1:963567/54‑1 (MQ=255)                                                                                                                        cTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTc                                                                                        >  2:963567/1‑54 (MQ=255)                                                                                                                        cTTCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACGGGAATTCGATGACAGTGCggg   >  1:612678/1‑139 (MQ=255)                                                                                                                         ttCTCGACACGGGTAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACGGGAATTCGATGACAGTGCGGGa  <  1:98718/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                               TGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGATAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGATGAAGAGTTAACGGGAATTCGATGACAGTGCGGGA  >  NC_000913/4325020‑4325278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑