Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,352,880	G→A	*99* (TAG→TAA)	ghoS →	antitoxin of GhoTS toxin‑antitoxin pair, endonuclease for ghoT mRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,352,880	0	G	A	100.0%	169.4 / NA	53	*99* (TAG→TAA)	ghoS	antitoxin of GhoTS toxin‑antitoxin pair, endonuclease for ghoT mRNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/31);  total (22/31)

TTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGT  >  NC_000913/4352746‑4353006                                                                                                                                       |                                                                                                                               ttCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCa                                                                                                                             >  1:509807/1‑138 (MQ=255) ttCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCa                                                                                                                             <  2:509807/138‑1 (MQ=255)  tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagg                                                                                                                           <  1:1271573/139‑1 (MQ=255)  tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagg                                                                                                                           <  1:816021/139‑1 (MQ=255)  tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATGATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagg                                                                                                                           <  2:916280/139‑1 (MQ=255)   cGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                          <  2:354687/139‑1 (MQ=255)             aCATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATaagaa                                                                                                                <  1:402148/139‑1 (MQ=255)              cATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATaagaag                                                                                                               <  2:1099424/139‑1 (MQ=255)                            aGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGTATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                          <  2:21303/114‑1 (MQ=255)                            aGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                          >  1:21303/1‑114 (MQ=255)                            aGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATgg                                                                                                 >  1:12726/1‑139 (MQ=255)                              tGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                               <  1:329532/139‑1 (MQ=255)                              tGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                               <  1:551166/139‑1 (MQ=255)                                                      cAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAAttttt                                                                       <  1:189206/139‑1 (MQ=255)                                                             ttAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                >  1:705651/1‑139 (MQ=255)                                                             ttAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                <  2:43228/139‑1 (MQ=255)                                                              tAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGAt                                                               <  1:664620/139‑1 (MQ=255)                                                               aGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGAtt                                                              >  2:960842/1‑139 (MQ=255)                                                                  cTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgt                                                           <  1:960842/139‑1 (MQ=255)                                                                    tACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgtgt                                                         >  1:275627/1‑139 (MQ=255)                                                                     aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                          <  1:1268140/73‑1 (MQ=255)                                                                     aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                          >  2:1268140/1‑73 (MQ=255)                                                                     aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTa                                                                                            >  2:323016/1‑103 (MQ=255)                                                                     aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTa                                                                                            <  1:323016/103‑1 (MQ=255)                                                                     aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGtgtg                                                        >  1:460287/1‑139 (MQ=255)                                                                               gATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCt                                              <  2:275627/139‑1 (MQ=255)                                                                               gATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCt                                              <  2:1243183/139‑1 (MQ=255)                                                                                aTTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTa                                                                                            >  2:892377/1‑92 (MQ=255)                                                                                aTTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTa                                                                                            <  1:892377/92‑1 (MQ=255)                                                                                          cAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                <  2:1028276/110‑1 (MQ=255)                                                                                          cAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                >  1:1028276/1‑110 (MQ=255)                                                                                          cAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATc                                   >  1:983972/1‑139 (MQ=255)                                                                                                     cAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTa                                                                                            <  2:860335/71‑1 (MQ=255)                                                                                                     cAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTa                                                                                            >  1:860335/1‑71 (MQ=255)                                                                                                     cAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                >  1:781615/1‑99 (MQ=255)                                                                                                     cAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                <  2:781615/99‑1 (MQ=255)                                                                                                       gTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAACATg                                                                                                  >  2:878295/1‑63 (MQ=255)                                                                                                       gTTGATGACTATTTCCGTCAGTTTTACAAGTAAACAGGAAGGATAAGAAGAACATAAAAAATg                                                                                                  <  1:878295/63‑1 (MQ=255)                                                                                                        ttGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAagaaga                                                                                                              >  1:898762/1‑50 (MQ=255)                                                                                                        ttGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAagaaga                                                                                                              <  2:898762/50‑1 (MQ=255)                                                                                                        ttGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGAt                                                               >  2:376721/1‑97 (MQ=255)                                                                                                        ttGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGAt                                                               <  1:376721/97‑1 (MQ=255)                                                                                                        ttGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCa                                        <  2:179642/120‑1 (MQ=255)                                                                                                        ttGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCa                                        >  1:179642/1‑120 (MQ=255)                                                                                                          gatgaCTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                               <  2:116532/63‑1 (MQ=255)                                                                                                          gatgaCTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                               >  1:116532/1‑63 (MQ=255)                                                                                                                  tttCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGAt                                                               >  1:204040/1‑87 (MQ=255)                                                                                                                  tttCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGAt                                                               <  2:204040/87‑1 (MQ=255)                                                                                                                          aGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTAtt                                                                                          >  1:452848/1‑52 (MQ=255)                                                                                                                          aGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTAtt                                                                                          <  2:452848/52‑1 (MQ=255)                                                                                                                           gTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGt  <  1:1205587/139‑1 (MQ=255)                                                                                                                            ttttACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                               >  2:631041/1‑45 (MQ=255)                                                                                                                            ttttACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                               <  1:631041/45‑1 (MQ=255)                                                                                                                                       |                                                                                                                               TTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGT  >  NC_000913/4352746‑4353006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑