Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,357,276	C→T	G448S (GGC→AGC)	cadA ←	lysine decarboxylase, acid‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,357,276	0	C	T	100.0%	274.4 / NA	85	G448S (GGC→AGC)	cadA	lysine decarboxylase, acid‑inducible
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/40);  total (45/40)

TGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCCATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTTCATCATCGCCGCAGCGGTTTCAGTGGACGCCACGATACCGTA  >  NC_000913/4357144‑4357411                                                                                                                                     |                                                                                                                                        tGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGa                                                                                                                                   >  1:498545/1‑139 (MQ=255)   aCTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGAtt                                                                                                                                 <  1:613912/139‑1 (MQ=255)             ggTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAg                                                                                                                       >  1:488897/1‑139 (MQ=255)             ggTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGCCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAg                                                                                                                       >  1:476402/1‑139 (MQ=255)              gTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGa                                                                                                                      <  2:1288261/139‑1 (MQ=255)                     tACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGa                                                                                                               <  1:506260/139‑1 (MQ=255)                     tACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGa                                                                                                               <  2:498545/139‑1 (MQ=255)                      aCATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGAt                                                                                                              <  2:513702/139‑1 (MQ=255)                        aTGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGAtct                                                                                                            >  2:1127217/1‑139 (MQ=255)                         tgtgCTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGAtctc                                                                                                           >  1:1018324/1‑139 (MQ=255)                             cTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTa                                                                                                       >  1:1268747/1‑139 (MQ=255)                             cTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTa                                                                                                       >  1:1173758/1‑139 (MQ=255)                             cTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTa                                                                                                       >  1:562752/1‑139 (MQ=255)                             cTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTa                                                                                                       <  2:409576/139‑1 (MQ=255)                             cTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTa                                                                                                       <  1:88311/139‑1 (MQ=255)                             cTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTa                                                                                                       >  1:192915/1‑139 (MQ=255)                             cTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTa                                                                                                       >  2:1195139/1‑139 (MQ=255)                             cTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTACGTTCTCAGACGTTTGATCTCTTTa                                                                                                       >  2:1150445/1‑139 (MQ=255)                                 ttATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGa                                                                                                   >  2:542468/1‑139 (MQ=255)                                           ttttGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGc                                                                                         <  1:1127217/139‑1 (MQ=255)                                            tttGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCa                                                                                        >  2:1053056/1‑139 (MQ=255)                                               gAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGt                                                                                     <  1:448935/139‑1 (MQ=255)                                                    cGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGAtt                                                                                                                                 >  1:151124/1‑90 (MQ=255)                                                    cGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGAtt                                                                                                                                 <  2:151124/90‑1 (MQ=255)                                                      tGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAg                                                                              >  1:479232/1‑139 (MQ=255)                                                           ggTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCg                                                                         <  1:542468/139‑1 (MQ=255)                                                                tGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCa                                                                                                                        <  1:277020/87‑1 (MQ=255)                                                                tGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCa                                                                                                                        >  2:277020/1‑87 (MQ=255)                                                                tGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGc                                                                                         >  1:61297/1‑118 (MQ=255)                                                                tGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGc                                                                                         <  2:61297/118‑1 (MQ=255)                                                                 gTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGAtt                                                                                                                                 >  2:112701/1‑77 (MQ=255)                                                                 gTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGAtt                                                                                                                                 <  1:112701/77‑1 (MQ=255)                                                                 gTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACg                                                                                      <  2:425891/120‑1 (MQ=255)                                                                 gTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACg                                                                                      >  1:425891/1‑120 (MQ=255)                                                                  tCAGAACTCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCACAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCa                                                                  <  2:1088371/139‑1 (MQ=255)                                                                    aGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGa                                                                <  2:898072/139‑1 (MQ=255)                                                                      aaCGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACg                                                              >  1:414464/1‑139 (MQ=255)                                                                      aaCGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACg                                                              <  2:570931/139‑1 (MQ=255)                                                                         gCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCtt                                                           >  1:986805/1‑139 (MQ=255)                                                                            gCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTAcc                                                        >  1:1005724/1‑139 (MQ=255)                                                                                ccAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCa                                                    <  2:479232/139‑1 (MQ=255)                                                                                 cAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCAt                                                   <  2:1005724/139‑1 (MQ=255)                                                                                  aGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCtt                                                                                                         <  2:31868/84‑1 (MQ=255)                                                                                  aGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCtt                                                                                                         >  1:31868/1‑84 (MQ=255)                                                                                          gTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTtct                                                                                                                          >  1:605298/1‑59 (MQ=255)                                                                                          gTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTtct                                                                                                                          <  2:605298/59‑1 (MQ=255)                                                                                          gTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTAc                                                                                                      >  2:1211595/1‑79 (MQ=255)                                                                                          gTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTAc                                                                                                      <  1:1211595/79‑1 (MQ=255)                                                                                              tATCGATATGATCCGGCTGCCATACATCAAAGAACCATCTATCTGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATc                                                                                           >  1:866446/1‑86 (MQ=255)                                                                                              tATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCGTTACGGAATTTGATc                                                                                           <  2:866446/86‑1 (MQ=255)                                                                                                  gATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTtc                                           <  1:719828/130‑1 (MQ=255)                                                                                                  gATATGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTTCATCATcgcc                                  <  2:1268747/139‑1 (MQ=255)                                                                                                  gATATGATCCGGCTGCCAAACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTtc                                           >  2:719828/1‑130 (MQ=255)                                                                                                   atatGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACg                                                                                      <  1:172655/86‑1 (MQ=255)                                                                                                   atatGATCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACg                                                                                      >  2:172655/1‑86 (MQ=255)                                                                                                        aTCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTTCATCATCGCCGCAGCg                            >  2:714266/1‑139 (MQ=255)                                                                                                         tCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGAt                                                                                                              <  1:1177733/56‑1 (MQ=255)                                                                                                         tCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGAt                                                                                                              >  2:1177733/1‑56 (MQ=255)                                                                                                         tCCGGCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCGATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTTCATCATCGCCGCAGCgg                           >  2:1185798/1‑139 (MQ=255)                                                                                                            ggCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGa                                                                                                                      <  2:438734/45‑1 (MQ=255)                                                                                                            ggCTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGa                                                                                                                      >  1:438734/1‑45 (MQ=255)                                                                                                              cTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGa                                                                                                               <  1:1182929/50‑1 (MQ=255)                                                                                                              cTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGa                                                                                                               >  2:1182929/1‑50 (MQ=255)                                                                                                              cTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCAt                                                   <  2:1045574/110‑1 (MQ=255)                                                                                                              cTGCCATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCAt                                                   >  1:1045574/1‑110 (MQ=255)                                                                                                                 ccATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTTCATCATCGCCGCAGCGGTTTCAGTg                   <  2:488897/139‑1 (MQ=255)                                                                                                                  cATACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTTCATCATCGCCGCAGCGGTTTCAGTgg                  >  1:355188/1‑139 (MQ=255)                                                                                                                   aTACATCAAAGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTTCATCATCGCCGCAGCGGTTTCAGTGGa                 <  2:993001/139‑1 (MQ=255)                                                                                                                           aaGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGa                                                                                                               <  1:1219371/37‑1 (MQ=255)                                                                                                                           aaGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGa                                                                                                               >  2:1219371/1‑37 (MQ=255)                                                                                                                           aaGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACg                                                                                      >  2:605132/1‑62 (MQ=255)                                                                                                                           aaGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACg                                                                                      <  1:605132/62‑1 (MQ=255)                                                                                                                           aaGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCg                                                                         >  2:873735/1‑75 (MQ=255)                                                                                                                           aaGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCg                                                                         <  1:873735/75‑1 (MQ=255)                                                                                                                           aaGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTTCATCATCGCCGCa                               <  2:902590/117‑1 (MQ=255)                                                                                                                           aaGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTTCATCATCGCCGCa                               >  1:902590/1‑117 (MQ=255)                                                                                                                            aGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCGATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTTCATCATCGCCGCAGCGGTTTCAGTGGACGCCACGAt        <  1:1185798/139‑1 (MQ=255)                                                                                                                            aGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTAc                                                         >  2:62925/1‑90 (MQ=255)                                                                                                                            aGAACCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTAc                                                         <  1:62925/90‑1 (MQ=255)                                                                                                                              aaCCAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTTCATCATCGCCGCAGCGGTTTCAGTGGACGCCACGATAc      >  1:404977/1‑139 (MQ=255)                                                                                                                                 cAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGc                                                                                         <  1:996082/53‑1 (MQ=255)                                                                                                                                 cAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGc                                                                                         >  2:996082/1‑53 (MQ=255)                                                                                                                                 cAGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTTCATCATCGCCGCAGCGGTTTCAGTGGACGCCACGATACCGt   >  1:327895/1‑139 (MQ=255)                                                                                                                                  aGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTTCATCATCGCCGCAGCGGTTTCAGTGGACGCCACGATACCGTa  >  1:47247/1‑139 (MQ=255)                                                                                                                                  aGCTATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTTCATCATCGCAGCAGCGGTTTCAGTGGACGCCACGATACCGTa  >  1:575422/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                        TGACTTTGATCGGGTCAAGATACATGTGCTCGTTATCGATGTTTTTGAAGCCGTGCCAGGTGCTGTCAGAACGCAGCGGCCAGCATTCAGTCGTATCGATATGATCCGGCTGCCATACATCAAAGAACCAGCCATCAGATTCCGTTCTCAGACGTTTGATCTCTTTACGGAATTTGATCGCACGTTCAATAGAACCGTTGATCAGACGCTTACCTGCATTGCCTTTCATCATCGCCGCAGCGGTTTCAGTGGACGCCACGATACCGTA  >  NC_000913/4357144‑4357411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑