Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,386,872	C→T	A833A (GCG→GCA)	mscM ←	mechanosensitive channel protein, miniconductance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,386,872	0	C	T	100.0%	172.1 / NA	54	A833A (GCG→GCA)	mscM	mechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/28);  total (26/28)

TTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGG  >  NC_000913/4386737‑4387001                                                                                                                                        |                                                                                                                                  tttGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgcactgca                                                                                                                                <  2:1148722/139‑1 (MQ=255)        atggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATGGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAggg                                                                                                                         >  2:908926/1‑139 (MQ=255)        atggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAggg                                                                                                                         >  2:976998/1‑139 (MQ=255)        atggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAggg                                                                                                                         >  1:229980/1‑139 (MQ=255)           gtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTaa                                                                                                                      >  2:1078883/1‑139 (MQ=255)                        aaCCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAAt                                                                                                                     >  1:206611/1‑127 (MQ=255)                        aaCCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAAt                                                                                                                     <  2:206611/127‑1 (MQ=255)                            cGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                     <  2:635978/139‑1 (MQ=255)                              tACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTAc                                                                                                   >  2:687966/1‑139 (MQ=255)                               aCCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACg                                                                                                  <  2:441905/139‑1 (MQ=255)                                 ccGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc                                                                                                <  2:154550/139‑1 (MQ=255)                                 ccGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc                                                                                                <  1:529436/139‑1 (MQ=255)                                   ggCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACg                                                                                                  <  2:567743/135‑1 (MQ=255)                                   ggCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACg                                                                                                  >  1:567743/1‑135 (MQ=255)                                   ggCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCt                                                                                              <  1:976998/139‑1 (MQ=255)                                     cGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGt                                                                                            >  2:911577/1‑139 (MQ=255)                                     cGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGt                                                                                            >  2:945929/1‑139 (MQ=255)                                      gTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTa                                                                                           <  1:908926/139‑1 (MQ=255)                                      gTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTa                                                                                           <  1:1078883/139‑1 (MQ=255)                                          aaTCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGt                                                                                       <  2:183934/139‑1 (MQ=255)                                            tCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTgg                                                                                     <  2:999435/139‑1 (MQ=255)                                                      gcAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                     <  1:783019/113‑1 (MQ=255)                                                      gcAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGAACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                     >  2:783019/1‑113 (MQ=255)                                                       cAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATcc                                                                          >  1:409889/1‑139 (MQ=255)                                                              gCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCg                                                                   <  1:911577/139‑1 (MQ=255)                                                               ccAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCTAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGa                                                                  >  2:528192/1‑139 (MQ=255)                                                                 aGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGaaa                                                                <  2:170585/139‑1 (MQ=255)                                                                           gcgcGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAg                                                      <  1:1239099/139‑1 (MQ=255)                                                                            cgcgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGAc                                                                                                          >  1:101242/1‑86 (MQ=255)                                                                            cgcgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGAc                                                                                                          <  2:101242/86‑1 (MQ=255)                                                                             gcgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGc                                                                                                 >  1:186914/1‑94 (MQ=255)                                                                             gcgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGc                                                                                                 <  2:186914/94‑1 (MQ=255)                                                                             gcgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGa                                                    >  2:152730/1‑139 (MQ=255)                                                                              cgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGaa                                                   >  2:222308/1‑139 (MQ=255)                                                                              cgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGaa                                                   >  1:147713/1‑139 (MQ=255)                                                                              cgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGaa                                                   >  1:401483/1‑139 (MQ=255)                                                                              cgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGaa                                                   >  2:218710/1‑139 (MQ=255)                                                                                   aaGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGa                                              <  2:229980/139‑1 (MQ=255)                                                                                               aGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCTAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGa                                  <  1:528192/139‑1 (MQ=255)                                                                                                    cGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCt                             <  2:35676/139‑1 (MQ=255)                                                                                                    cGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCt                             <  1:222308/139‑1 (MQ=255)                                                                                                    cGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCt                             <  1:408010/139‑1 (MQ=255)                                                                                                      tggtgATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGa                           >  1:103909/1‑139 (MQ=255)                                                                                                              atAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGAc                          <  2:727990/132‑1 (MQ=255)                                                                                                              atAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGAc                          >  1:727990/1‑132 (MQ=255)                                                                                                              atAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGca                   <  2:147713/139‑1 (MQ=255)                                                                                                                   cacCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATg              >  1:459945/1‑139 (MQ=255)                                                                                                                        gAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTg                                                                                      >  2:557041/1‑62 (MQ=255)                                                                                                                        gAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTg                                                                                      <  1:557041/62‑1 (MQ=255)                                                                                                                         aaTGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGAc                                                                              >  1:766282/1‑69 (MQ=255)                                                                                                                         aaTGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGAc                                                                              <  2:766282/69‑1 (MQ=255)                                                                                                                         aaTGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCTGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGaa        >  2:1143266/1‑139 (MQ=255)                                                                                                                               aaTCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAgg  <  1:152730/139‑1 (MQ=255)                                                                                                                                   aGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc                                                                                                >  2:1041821/1‑41 (MQ=255)                                                                                                                                   aGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc                                                                                                <  1:1041821/41‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  TTTGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGG  >  NC_000913/4386737‑4387001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑