Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,447,891 G→A *1260* (TAG→TAA tamB → translocation and assembly module for autotransporter export, inner membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,447,8910GA100.0% 379.3 / NA 116*1260* (TAG→TAAtamBtranslocation and assembly module for autotransporter export, inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (62/54);  total (62/54)

Only 100 of 116 total aligned reads displayed.

CAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCG  >  NC_000913/4447754‑4448023
                                                                                                                                         |                                                                                                                                    
              cGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGt                                                                                                                      >  2:670162/1‑139 (MQ=255)
              cGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGt                                                                                                                      >  2:508315/1‑139 (MQ=255)
                    tataTTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACgg                                                                                                                <  2:375248/139‑1 (MQ=255)
                            aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTAc                                                                                                        >  1:490966/1‑139 (MQ=255)
                            aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTAc                                                                                                        >  1:1255198/1‑139 (MQ=255)
                              tctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGc                                                                                                      >  2:18650/1‑139 (MQ=255)
                                        cacTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAg                                                                                            >  1:1273755/1‑139 (MQ=255)
                                          cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                          >  1:1015180/1‑139 (MQ=255)
                                          cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                          >  2:1250676/1‑139 (MQ=255)
                                          cTCACGTTACGTTATCGCCTGATGCATAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                          >  2:631547/1‑139 (MQ=255)
                                            cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                        <  2:657008/139‑1 (MQ=255)
                                            cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                        <  1:89211/139‑1 (MQ=255)
                                            cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                        <  2:754377/139‑1 (MQ=255)
                                            cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                        >  1:551204/1‑139 (MQ=255)
                                            cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                        >  1:1293267/1‑139 (MQ=255)
                                            cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                        <  1:460254/139‑1 (MQ=255)
                                              cgttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACa                                                                                      >  2:340108/1‑139 (MQ=255)
                                              cgttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACa                                                                                      >  2:1046965/1‑139 (MQ=255)
                                               gttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAg                                                                                     >  2:112648/1‑139 (MQ=255)
                                                  acgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCa                                                                                  >  2:91345/1‑139 (MQ=255)
                                                   cgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCAc                                                                                 <  2:1273755/139‑1 (MQ=255)
                                                        tCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAAt                                                                                                                                  <  2:473783/85‑1 (MQ=255)
                                                        tCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAAt                                                                                                                                  >  1:473783/1‑85 (MQ=255)
                                                        tCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                                            <  1:670162/139‑1 (MQ=255)
                                                             tGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCa                                                                       <  2:1129683/139‑1 (MQ=255)
                                                               aTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTc                                                                                   <  1:1074402/125‑1 (MQ=255)
                                                               aTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAAt                                                                     >  1:571683/1‑139 (MQ=255)
                                                                  cgtAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACGGGTCTTCGCTATCAGTTGGAGTTTTAACAAGGCGGATAGTTGTCTACGGCAGTTTACGACACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                  <  2:430239/137‑1 (MQ=255)
                                                                      aGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGt                                                              >  1:259399/1‑139 (MQ=255)
                                                                           tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAc                                                                         >  2:677900/1‑123 (MQ=255)
                                                                           tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAc                                                                         <  1:677900/123‑1 (MQ=255)
                                                                           tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTg                                                         >  1:232163/1‑139 (MQ=255)
                                                                                ggAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACgg                                                                                                                >  2:156715/1‑79 (MQ=255)
                                                                                ggAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACgg                                                                                                                <  1:156715/79‑1 (MQ=255)
                                                                                ggAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                                                    <  1:1232772/107‑1 (MQ=255)
                                                                                ggAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                                                    >  2:1232772/1‑107 (MQ=255)
                                                                                 gAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                        <  1:1200881/102‑1 (MQ=255)
                                                                                 gAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                        >  2:1200881/1‑102 (MQ=255)
                                                                                 gAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAt                                                   >  1:551284/1‑139 (MQ=255)
                                                                                 gAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAt                                                   <  1:985449/139‑1 (MQ=255)
                                                                                  aaGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                          <  1:427833/99‑1 (MQ=255)
                                                                                  aaGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                          >  2:427833/1‑99 (MQ=255)
                                                                                   aGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAttt                                                 <  2:259399/139‑1 (MQ=255)
                                                                                     ccGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCg                                                                                                                               <  1:1222592/59‑1 (MQ=255)
                                                                                     ccGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCg                                                                                                                               >  2:1222592/1‑59 (MQ=255)
                                                                                     ccGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAAt                                                                     >  2:435251/1‑117 (MQ=255)
                                                                                     ccGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAAt                                                                     <  1:435251/117‑1 (MQ=255)
                                                                                     ccGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCa                                                                <  2:1060127/122‑1 (MQ=255)
                                                                                     ccGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCa                                                                >  1:1060127/1‑122 (MQ=255)
                                                                                     ccGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                                               >  2:1280775/1‑139 (MQ=255)
                                                                                      catgtCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                        >  2:6487/3‑97 (MQ=255)
                                                                                      cGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                        <  1:1186015/97‑1 (MQ=255)
                                                                                       gtgtCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGt                                             <  1:719741/139‑1 (MQ=255)
                                                                                       gtgtCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGt                                             >  2:309812/1‑139 (MQ=255)
                                                                                        tgtCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGttt                                                                                                          >  2:22512/1‑77 (MQ=255)
                                                                                        tgtCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGttt                                                                                                          <  1:22512/77‑1 (MQ=255)
                                                                                        tgtCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTa                                            <  2:571683/139‑1 (MQ=255)
                                                                                         gtCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTAt                                           <  1:91345/139‑1 (MQ=255)
                                                                                                 tAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAAGGCCCAGTTACTGGGCGATTTCAGTATCGATAACt                                   <  1:1221057/139‑1 (MQ=255)
                                                                                                   gACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                 <  1:508315/139‑1 (MQ=255)
                                                                                                   gACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                 <  2:232163/139‑1 (MQ=255)
                                                                                                   gACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                 <  1:1061730/139‑1 (MQ=255)
                                                                                                    aCCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGc                                                                                                                                <  1:1261962/43‑1 (MQ=255)
                                                                                                    aCCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTa                                                                                                                   <  2:36762/56‑1 (MQ=255)
                                                                                                    aCCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTa                                                                                                                   >  1:36762/1‑56 (MQ=255)
                                                                                                    aCCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACgg                                                                                                                <  2:507207/59‑1 (MQ=255)
                                                                                                      cAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACTGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg                              <  1:687132/139‑1 (MQ=255)
                                                                                                      cAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg                              <  2:1293267/139‑1 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTGACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGtt                            >  1:1170518/1‑139 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCacaaa                                                                                                <  1:227598/71‑1 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCacaaa                                                                                                >  2:227598/1‑71 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGtt                            >  2:788472/1‑139 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGtt                            >  2:1108619/1‑139 (MQ=255)
                                                                                                        ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATAGATAACTACCAGtt                            >  2:1175633/1‑139 (MQ=255)
                                                                                                         gCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCg                                         <  1:563102/125‑1 (MQ=255)
                                                                                                           aCTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGta                         <  2:435482/139‑1 (MQ=255)
                                                                                                           aCTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATCTCAGTATCGATAACTACCAGTTGta                         <  2:551204/139‑1 (MQ=255)
                                                                                                              tggattGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGtata                       >  1:1290390/5‑139 (MQ=255)
                                                                                                               ggattGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTa                                            >  2:304533/4‑117 (MQ=255)
                                                                                                                   tGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                  <  2:715508/91‑1 (MQ=255)
                                                                                                                   tGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                  >  1:715508/1‑91 (MQ=255)
                                                                                                                   tGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTg                  <  2:1290390/139‑1 (MQ=255)
                                                                                                                   tGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTg                  <  1:309812/139‑1 (MQ=255)
                                                                                                                    gCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg                              >  1:212636/1‑126 (MQ=255)
                                                                                                                     ctctATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTggg                >  1:1006940/1‑139 (MQ=255)
                                                                                                                          tCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACt           >  2:1102039/1‑139 (MQ=255)
                                                                                                                              ttCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATcc       <  2:1255198/139‑1 (MQ=255)
                                                                                                                               tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                                      >  1:720127/1‑75 (MQ=255)
                                                                                                                               tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                                      <  2:720127/75‑1 (MQ=255)
                                                                                                                               tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCa      >  1:278608/1‑139 (MQ=255)
                                                                                                                               tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCa      >  2:1134604/1‑139 (MQ=255)
                                                                                                                                cGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAg     >  2:520082/1‑139 (MQ=255)
                                                                                                                                cGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAg     >  2:1260580/1‑139 (MQ=255)
                                                                                                                                  aGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                        <  1:1099167/54‑1 (MQ=255)
                                                                                                                                  aGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                        >  2:1099167/1‑54 (MQ=255)
                                                                                                                                  aGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                  >  1:891972/1‑76 (MQ=255)
                                                                                                                                  aGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                  <  2:891972/76‑1 (MQ=255)
                                                                                                                                   gTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGgcg  <  1:1059333/139‑1 (MQ=255)
                                                                                                                                    ttttAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                                      <  2:52046/86‑1 (MQ=255)
                                                                                                                                    ttttAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                                      >  1:52046/1‑86 (MQ=255)
                                                                                                                                         |                                                                                                                                    
CAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCG  >  NC_000913/4447754‑4448023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: