Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,565,966	C→T	*427* (TAG→TAA)	yjiN ←	zinc‑type alcohol dehydrogenase‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,565,966	0	C	T	100.0%	227.7 / NA	71	*427* (TAG→TAA)	yjiN	zinc‑type alcohol dehydrogenase‑like protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/32);  total (39/32)

TTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATT  >  NC_000913/4565859‑4566095                                                                                                            |                                                                                                                                  ttccCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGa                                                                                                    <  1:11246/139‑1 (MQ=255)             cGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                                        <  2:668084/139‑1 (MQ=255)             cGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                                        <  1:159545/139‑1 (MQ=255)             cGGGAGATAATGAAAAGAGG‑CACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGAGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCaa                                                                                       >  1:273547/1‑139 (MQ=255)                            agagGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATCGCCGAGGGGGAACAAGGCCGGGAGCTGCGCCAGCAAATATAAAATTAGcc                                                                         >  1:987778/1‑139 (MQ=255)                               ggCCACATTATTACCATTCTTTTAGTATGGTCTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                                         <  2:95418/120‑1 (MQ=255)                               ggCCACATTATTACCATTCTTTTAGTATGGTCTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                                         >  1:95418/1‑120 (MQ=255)                                gCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAg                                                                           <  1:344412/133‑1 (MQ=255)                                gCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAg                                                                           >  2:344412/1‑133 (MQ=255)                                   acaTTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATaaa                                                                  >  1:1128541/1‑137 (MQ=255)                                          taCCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAgg                                                                                                                    <  1:476457/82‑1 (MQ=255)                                          taCCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAgg                                                                                                                    >  2:476457/1‑82 (MQ=255)                                            ccATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                                         >  2:570797/1‑107 (MQ=255)                                            ccATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                                         <  1:570797/107‑1 (MQ=255)                                            ccATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                                        >  1:149736/1‑108 (MQ=255)                                            ccATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                                        <  2:149736/108‑1 (MQ=255)                                               ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGcc                                                                                                                        >  2:447307/1‑73 (MQ=255)                                               ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGcc                                                                                                                        <  1:447307/73‑1 (MQ=255)                                               ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAgg                                                                                                                    <  1:466204/77‑1 (MQ=255)                                               ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAgg                                                                                                                    >  2:466204/1‑77 (MQ=255)                                               ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                                        >  2:439427/1‑105 (MQ=255)                                               ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                                        <  1:439427/105‑1 (MQ=255)                                               ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAg                                                      >  2:944741/1‑139 (MQ=255)                                               ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAg                                                      >  1:1033933/1‑139 (MQ=255)                                                           ggTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGaa                                                                                                               <  1:195083/70‑1 (MQ=255)                                                           ggTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGaa                                                                                                               >  2:195083/1‑70 (MQ=255)                                                           ggTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGac                                          >  1:1136096/1‑139 (MQ=255)                                                            gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGcc                                                                                                                        <  1:145308/60‑1 (MQ=255)                                                            gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGcc                                                                                                                        >  2:145308/1‑60 (MQ=255)                                                            gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                                        <  1:5338/92‑1 (MQ=255)                                                            gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                                        >  2:5338/1‑92 (MQ=255)                                                            gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGaca                                         <  2:705449/139‑1 (MQ=255)                                                            gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGaca                                         >  1:705449/1‑139 (MQ=255)                                                             tttATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGacac                                        <  2:415200/139‑1 (MQ=255)                                                              ttATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACg                                       >  2:719553/1‑139 (MQ=255)                                                              ttATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACg                                       >  2:696419/1‑139 (MQ=255)                                                                  ttGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                                   >  2:322827/1‑139 (MQ=255)                                                                    gATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCg                                                    >  1:510277/1‑120 (MQ=255)                                                                    gATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCg                                                    <  2:510277/120‑1 (MQ=255)                                                                      tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                                         >  2:962905/1‑81 (MQ=255)                                                                      tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                                         >  1:1021254/1‑81 (MQ=255)                                                                      tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                                         <  2:1021254/81‑1 (MQ=255)                                                                      tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                                         <  1:962905/81‑1 (MQ=255)                                                                      tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                                   <  1:878629/103‑1 (MQ=255)                                                                      tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                                   >  2:878629/1‑103 (MQ=255)                                                                      tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACt                               >  1:217207/1‑139 (MQ=255)                                                                       ctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCg                                                                                                                       >  1:1084682/1‑50 (MQ=255)                                                                       ctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCg                                                                                                                       <  2:1084682/50‑1 (MQ=255)                                                                               cgGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCtt                      <  1:322827/139‑1 (MQ=255)                                                                               cgGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCtt                      >  2:1254612/1‑139 (MQ=255)                                                                                    aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                                   >  1:682545/1‑89 (MQ=255)                                                                                    aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                                   <  2:682545/89‑1 (MQ=255)                                                                                    aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGtt                                             <  2:1134080/111‑1 (MQ=255)                                                                                    aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGtt                                             >  1:1134080/1‑111 (MQ=255)                                                                                    aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg                 >  1:53696/1‑139 (MQ=255)                                                                                    aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg                 <  2:1033933/139‑1 (MQ=255)                                                                                          ttAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAGATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATGAACTGCAGATCTTTGc                   >  1:721326/1‑131 (MQ=255)                                                                                          ttAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAGATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGc                   <  2:721326/131‑1 (MQ=255)                                                                                            aGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                                        <  1:139435/60‑1 (MQ=255)                                                                                            aGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                                        >  2:139435/1‑60 (MQ=255)                                                                                            aGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAAt                                    <  1:1133983/112‑1 (MQ=255)                                                                                            aGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAAt                                    >  2:1133983/1‑112 (MQ=255)                                                                                                 ttCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTa    >  2:452158/1‑139 (MQ=255)                                                                                                 ttCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTa    <  1:452158/139‑1 (MQ=255)                                                                                                   cATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATATAATTAGCCCAATACAACCGCCAACCAGCg                                                    <  2:746277/89‑1 (MQ=255)                                                                                                   cATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCg                                                    >  1:746277/1‑89 (MQ=255)                                                                                                   cATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTAtt  >  2:1213373/1‑139 (MQ=255)                                                                                                       gATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg                 >  1:260543/1‑120 (MQ=255)                                                                                                       gATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg                 <  2:260543/120‑1 (MQ=255)                                                                                                        aTTTTTAAAAATTGCTGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAg                                                                           >  1:114573/1‑61 (MQ=255)                                                                                                        aTTTTTAAAAATTGCTGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAg                                                                           <  2:114573/61‑1 (MQ=255)                                                                                                            |                                                                                                                                  TTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATT  >  NC_000913/4565859‑4566095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑