Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,612,289	G→A	*54* (TAG→TAA)	ytjA →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,612,289	0	G	A	100.0%	57.4 / NA	19	*54* (TAG→TAA)	ytjA	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/10);  total (9/10)

TTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCG  >  NC_000913/4612156‑4612421                                                                                                                                      |                                                                                                                                     ttATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTc                                                                                                                                 <  2:384443/139‑1 (MQ=255)                        cTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATcc                                                                                                          <  1:5493/139‑1 (MQ=255)                               ttGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCa                                                                                                   <  2:910667/139‑1 (MQ=255)                                 ggtggtCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTTATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGt                                                                                                 >  1:1212225/1‑139 (MQ=255)                                   tggtCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATcc                                                                                                          <  1:46791/128‑1 (MQ=255)                                   tggtCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATcc                                                                                                          >  2:46791/1‑128 (MQ=255)                                                        aGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACt                                                                                       <  1:347072/126‑1 (MQ=255)                                                        aGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACt                                                                                       >  2:347072/1‑126 (MQ=255)                                                         ggCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGttt                                                                         >  2:400984/1‑139 (MQ=255)                                                             cAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGc                                                                     <  2:492300/139‑1 (MQ=255)                                                                               tcgtcgGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTa                                                   >  2:502332/1‑139 (MQ=255)                                                                                              tGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTc                                    <  1:502332/139‑1 (MQ=255)                                                                                                ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATcc                                                                                                          >  1:421373/1‑67 (MQ=255)                                                                                                ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATcc                                                                                                          <  2:421373/67‑1 (MQ=255)                                                                                                ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGa                                                                   <  2:344781/106‑1 (MQ=255)                                                                                                ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGa                                                                   >  1:344781/1‑106 (MQ=255)                                                                                                                   tGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCag               >  1:1083399/1‑139 (MQ=255)                                                                                                                   tGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTAAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCag               >  2:765595/1‑139 (MQ=255)                                                                                                                                aCCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCg  <  2:601596/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     TTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCG  >  NC_000913/4612156‑4612421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑