Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,913	A→G	E104G (GAA→GGA)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,913	0	A	G	100.0%	196.6 / NA	55	E104G (GAA→GGA)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/29);  total (26/29)

GCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGG  >  NC_000913/4619786‑4620048                                                                                                                                |                                                                                                                                        gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGgtg                                                                                                                              >  1:639788/1‑139 (MQ=255) gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGgtg                                                                                                                              <  1:1166541/139‑1 (MQ=255)                  ttCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAg                                                                                                             <  2:290925/139‑1 (MQ=255)                  ttCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAg                                                                                                             <  2:1218370/139‑1 (MQ=255)                            ttCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATAtt                                                                                                   >  2:630044/1‑139 (MQ=255)                               aTTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTtct                                                                                                >  2:363681/1‑139 (MQ=255)                                    ggcggGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTcc                                                                                                                           >  2:309794/1‑107 (MQ=255)                                    ggcggGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTcc                                                                                                                           <  1:309794/107‑1 (MQ=255)                                           aTGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCg                                                                                             <  1:1046146/130‑1 (MQ=255)                                           aTGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCg                                                                                             >  2:1046146/1‑130 (MQ=255)                                           aTGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGaaa                                                                                    >  1:165205/1‑139 (MQ=255)                                                  gCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCtt                                                                             <  1:39026/139‑1 (MQ=255)                                                      cGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCAATCACGAAAACAGCTTCCCg                                                                         >  1:1028986/1‑139 (MQ=255)                                                          gAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAag                                                                     <  2:639788/139‑1 (MQ=255)                                                              ttATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgct                                                                 <  1:363681/139‑1 (MQ=255)                                                              ttATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgct                                                                 <  2:101189/139‑1 (MQ=255)                                                               tATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCTGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                                <  1:562281/139‑1 (MQ=255)                                                               tATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                                <  2:165205/139‑1 (MQ=255)                                                                     cgcgTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTgg                                                                                                          <  2:396125/91‑1 (MQ=255)                                                                     cgcgTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTgg                                                                                                          >  1:396125/1‑91 (MQ=255)                                                                       cgTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATaa                                                        >  1:921805/1‑139 (MQ=255)                                                                                gggCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGa                                               <  1:392337/139‑1 (MQ=255)                                                                                   cgcACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAAcg                                            >  1:472401/1‑139 (MQ=255)                                                                                   cgcACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAAcg                                            >  1:643617/1‑139 (MQ=255)                                                                                          aaTGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCgg                                                                                                                        >  1:525197/1‑56 (MQ=255)                                                                                          aaTGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCgg                                                                                                                        <  2:525197/56‑1 (MQ=255)                                                                                                    ggTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGtt                           >  2:499988/1‑139 (MQ=255)                                                                                                        aaGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCt                       <  1:1262196/139‑1 (MQ=255)                                                                                                               cccGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTgggg                                                                                                        <  1:1238442/51‑1 (MQ=255)                                                                                                               cccGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTgggg                                                                                                        >  2:1238442/1‑51 (MQ=255)                                                                                                               cccGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgct                                                                 <  1:1122772/90‑1 (MQ=255)                                                                                                               cccGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgct                                                                 >  2:1122772/1‑90 (MQ=255)                                                                                                               cccGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATa                                                         >  2:249076/1‑98 (MQ=255)                                                                                                               cccGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATa                                                         <  1:249076/98‑1 (MQ=255)                                                                                                               cccGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCt                       <  1:400123/132‑1 (MQ=255)                                                                                                               cccGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCt                       >  2:400123/1‑132 (MQ=255)                                                                                                                   tCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGCGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGtt                           >  2:1193932/1‑124 (MQ=255)                                                                                                                   tCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGtt                           <  1:1193932/124‑1 (MQ=255)                                                                                                                    cTGGTCACTGGGGACTTGCCGGTGTCCCGGTTCTGTTTGACTGGGGATATTTCTCCGATCACGAAAACAGCTCCCCGCAAGAGCTGCTGGATAAACTGGCCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa           >  1:787694/1‑139 (MQ=255)                                                                                                                    cTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGa                                                                                                              >  1:362845/1‑40 (MQ=255)                                                                                                                    cTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGa                                                                                                              <  2:362845/40‑1 (MQ=255)                                                                                                                    cTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCgg                    <  2:1161398/130‑1 (MQ=255)                                                                                                                    cTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCgg                    >  1:1161398/1‑130 (MQ=255)                                                                                                                    cTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa           <  1:630044/139‑1 (MQ=255)                                                                                                                    cTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa           <  1:499988/139‑1 (MQ=255)                                                                                                                    cTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCACAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa           <  1:159133/139‑1 (MQ=255)                                                                                                                      ggTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAg                                                                                                             <  2:997124/39‑1 (MQ=255)                                                                                                                      ggTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAg                                                                                                             >  1:997124/1‑39 (MQ=255)                                                                                                                      ggTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGt                                                                                                            >  1:954626/1‑40 (MQ=255)                                                                                                                      ggTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGt                                                                                                            <  2:954626/40‑1 (MQ=255)                                                                                                                        tCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGa                                                                                                       >  2:594647/1‑43 (MQ=255)                                                                                                                        tCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGa                                                                                                       <  1:594647/43‑1 (MQ=255)                                                                                                                         cACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCg                                                                                             <  1:1283211/52‑1 (MQ=255)                                                                                                                         cACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCg                                                                                             >  2:1283211/1‑52 (MQ=255)                                                                                                                           cTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTcc    >  2:200233/1‑139 (MQ=255)                                                                                                                             ggggAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCgg  <  2:921805/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                        GCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGG  >  NC_000913/4619786‑4620048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑