Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 372,966 A→G T16A (ACC→GCC)  mhpF → acetaldehyde‑CoA dehydrogenase II, NAD‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913372,9660AG100.0% 141.1 / NA 40T16A (ACC→GCC) mhpFacetaldehyde‑CoA dehydrogenase II, NAD‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/22);  total (18/22)

CGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGA  >  NC_000913/372836‑373098
                                                                                                                                  |                                                                                                                                    
cGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCtg                                                                                                                              <  2:81678/139‑1 (MQ=255)
 gggCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCtga                                                                                                                             >  1:459960/1‑139 (MQ=255)
                         tGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTc                                                                                                     <  1:1233044/139‑1 (MQ=255)
                          gAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCa                                                                                                    <  2:459960/139‑1 (MQ=255)
                                 tAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTc                                                                                                     <  1:1083225/131‑1 (MQ=255)
                                 tAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTc                                                                                                     >  2:1083225/1‑131 (MQ=255)
                                               gcGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGAt                                                                               >  2:14249/1‑139 (MQ=255)
                                                  aCATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGt                                                                            <  1:836695/139‑1 (MQ=255)
                                                     ttttCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTgg                                                                         <  2:1218535/139‑1 (MQ=255)
                                                         cAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCAtt                                                                     <  1:14249/139‑1 (MQ=255)
                                                         cAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCAtt                                                                     <  1:405187/139‑1 (MQ=255)
                                                                  ccccAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAg                                                            >  2:385112/1‑139 (MQ=255)
                                                                            aaGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTc                                                  >  1:259844/1‑139 (MQ=255)
                                                                            aaGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTc                                                  >  2:743041/1‑139 (MQ=255)
                                                                             aGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGt                                                                                                            <  2:587185/80‑1 (MQ=255)
                                                                             aGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGt                                                                                                            >  1:587185/1‑80 (MQ=255)
                                                                                cTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGgc                                              >  2:238450/1‑139 (MQ=255)
                                                                                   tCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGgcgcg                                           <  1:743041/139‑1 (MQ=255)
                                                                                       gAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGcc                                       <  1:238450/139‑1 (MQ=255)
                                                                                          tAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGt                                                                            >  2:1139966/1‑99 (MQ=255)
                                                                                          tAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGt                                                                            <  1:1139966/99‑1 (MQ=255)
                                                                                             gCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGt                                 >  1:131561/1‑139 (MQ=255)
                                                                                                  aaGTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATggg                            >  1:338840/1‑139 (MQ=255)
                                                                                                    gTCGCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCg                          >  2:1286561/1‑139 (MQ=255)
                                                                                                       gCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTg                                                                                 <  1:716434/81‑1 (MQ=255)
                                                                                                       gCCATTATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTg                                                                                 >  2:716434/1‑81 (MQ=255)
                                                                                                           ttATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGccac                   <  1:1286561/139‑1 (MQ=255)
                                                                                                           ttATCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGccac                   <  1:33404/139‑1 (MQ=255)
                                                                                                              tCGGTTCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCcaccac                <  2:338840/139‑1 (MQ=255)
                                                                                                                   tCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGAt                                                                                        >  1:591104/1‑62 (MQ=255)
                                                                                                                   tCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGAt                                                                                        <  2:591104/62‑1 (MQ=255)
                                                                                                                   tCTGGCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATg           >  2:27214/1‑139 (MQ=255)
                                                                                                                      ggCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAg        >  1:815954/1‑139 (MQ=255)
                                                                                                                       gCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAgg       <  1:385112/139‑1 (MQ=255)
                                                                                                                       gCAACATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAgg       <  2:131561/139‑1 (MQ=255)
                                                                                                                           cATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGt                                                   <  2:476639/91‑1 (MQ=255)
                                                                                                                           cATTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGt                                                   >  1:476639/1‑91 (MQ=255)
                                                                                                                            aTTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAg                                     <  1:1211494/104‑1 (MQ=255)
                                                                                                                            aTTGGTGCCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGa  <  1:379169/139‑1 (MQ=255)
                                                                                                                            aTTGGTGCCGATCTGATGATTAAAATTTTGCATCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAg                                     >  2:1211494/1‑104 (MQ=255)
                                                                                                                                  |                                                                                                                                    
CGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCAAGCGCAGCCCCAAAAGGAAGTCTGTCATGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGA  >  NC_000913/372836‑373098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: