Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 375,307 C→T intergenic (+426/‑152) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913375,3070CT100.0% 52.7 / NA 18intergenic (+426/‑152)mhpE/mhpT4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/11);  total (7/11)

GAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTG  >  NC_000913/375175‑375436
                                                                                                                                    |                                                                                                                                 
gagaATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCa                                                                                                                             >  1:152513/1‑139 (MQ=25)
                                           cATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGt                                                                                  <  2:741219/139‑1 (MQ=255)
                                                 cgcACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGcc                                                                            >  2:37532/1‑139 (MQ=255)
                                                        cTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACAttt                                                                     >  1:812095/1‑139 (MQ=255)
                                                        cTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACAttt                                                                     <  2:812095/139‑1 (MQ=255)
                                                            cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGcc                                                                 <  1:37532/139‑1 (MQ=255)
                                                                tGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTa                                                             >  1:1110612/1‑139 (MQ=255)
                                                                   ggcgTAAACGCCTTATCCGGCTTACGGAGGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                                                          <  2:840211/139‑1 (MQ=255)
                                                                   ggcgTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                                                          <  2:1166548/139‑1 (MQ=255)
                                                                                  tCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                                                          <  1:615182/124‑1 (MQ=255)
                                                                                  tCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                                                          >  2:615182/1‑124 (MQ=255)
                                                                                                ggCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCt                             <  1:27321/139‑1 (MQ=255)
                                                                                                  cgcgGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTgg                           <  1:404056/139‑1 (MQ=255)
                                                                                                              tAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGt               <  2:856866/139‑1 (MQ=255)
                                                                                                                  ccTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGt                                                                                  >  2:505255/1‑68 (MQ=255)
                                                                                                                  ccTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGt                                                                                  <  1:505255/68‑1 (MQ=255)
                                                                                                                    tGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGAt         <  1:857495/139‑1 (MQ=255)
                                                                                                                           aCGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTg  >  2:1146568/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                 
GAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTG  >  NC_000913/375175‑375436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: