Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	37,903	C→T	D405N (GAC→AAC)	caiB ←	crotonobetainyl CoA:carnitine CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	37,903	0	C	T	100.0%	169.8 / NA	53	D405N (GAC→AAC)	caiB	crotonobetainyl CoA:carnitine CoA transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/25);  total (28/25)

CGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGTTA  >  NC_000913/37774‑38030                                                                                                                                  |                                                                                                                                cGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACttt                                                                                                                        >  2:131102/1‑139 (MQ=255) cGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACt                                                                                                                          >  2:590850/1‑137 (MQ=255) cGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACt                                                                                                                          <  1:590850/137‑1 (MQ=255)                   ttGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGAc                                                                                                      <  1:45139/139‑1 (MQ=255)                           gATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCt                                                                                              >  2:831535/1‑139 (MQ=255)                                 gtCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCt                                                                                                                               >  2:669475/1‑100 (MQ=255)                                 gtCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCt                                                                                                                               <  1:669475/100‑1 (MQ=255)                                 gtCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAAt                                                                                          >  2:1065216/1‑137 (MQ=255)                                 gtCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAAt                                                                                          <  1:1065216/137‑1 (MQ=255)                                 gtCCGCCAATGATATACATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGt                                                                                        >  2:865931/1‑139 (MQ=255)                                     gCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGtt                                                                                    <  2:433051/139‑1 (MQ=255)                                      ccAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGttt                                                                                   >  2:1176812/1‑139 (MQ=255)                                               tCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCt                                                                              >  1:47608/1‑135 (MQ=255)                                               tCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCt                                                                              <  2:47608/135‑1 (MQ=255)                                               tCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAg                                                                          >  1:651935/1‑139 (MQ=255)                                                     gCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGata                                                                    >  2:519590/1‑139 (MQ=255)                                                      cACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCttt                                                                                                            <  1:25/98‑1 (MQ=255)                                                      cACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCttt                                                                                                            >  2:25/1‑98 (MQ=255)                                                      cACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGtttt                                                                                  <  2:1190500/124‑1 (MQ=255)                                                      cACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGtttt                                                                                  >  1:1190500/1‑124 (MQ=255)                                                         ctctATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCt                                                                                                                               <  1:372443/76‑1 (MQ=255)                                                           ctATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTc                                                              >  1:788428/1‑139 (MQ=255)                                                              tCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACt                                                                                                                          <  1:369020/76‑1 (MQ=255)                                                              tCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACt                                                                                                                          >  2:369020/1‑76 (MQ=255)                                                              tCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTg                                                                             <  1:104245/121‑1 (MQ=255)                                                              tCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTg                                                                             >  2:104245/1‑121 (MQ=255)                                                                   ttttGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGc                                                      >  1:1040485/1‑139 (MQ=255)                                                                     ttGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGAc                                                                                                                <  1:843648/79‑1 (MQ=255)                                                                     ttGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCGCAACTTTGGCCAGAc                                                                                                                >  2:843648/1‑79 (MQ=255)                                                                      tGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGc                                                   <  2:1040485/139‑1 (MQ=255)                                                                       gTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGcc                                                  <  2:822092/139‑1 (MQ=255)                                                                       gTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGcc                                                  <  1:131102/139‑1 (MQ=255)                                                                        ttCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGAc                                                                                                                <  1:1274037/76‑1 (MQ=255)                                                                        ttCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGAc                                                                                                                >  2:1274037/1‑76 (MQ=255)                                                                        ttCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGcc                                                  <  1:670582/138‑1 (MQ=255)                                                                        ttCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGcc                                                  >  2:670582/1‑138 (MQ=255)                                                                        ttCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCg                                                 >  1:116235/1‑139 (MQ=255)                                                                            tttGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACTCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTc                                             <  1:865931/139‑1 (MQ=255)                                                                            tttGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTc                                             <  2:575235/139‑1 (MQ=255)                                                                                          ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACt                                                                                                                          <  2:1265831/48‑1 (MQ=255)                                                                                          ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACt                                                                                                                          >  1:1265831/1‑48 (MQ=255)                                                                                          ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTc                                             <  1:753791/125‑1 (MQ=255)                                                                                          ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTc                                             >  2:753791/1‑125 (MQ=255)                                                                                                           ccGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCaa                                                                                                   <  1:672461/54‑1 (MQ=255)                                                                                                           ccGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCaa                                                                                                   >  2:672461/1‑54 (MQ=255)                                                                                                           ccGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGa                >  2:1007251/1‑137 (MQ=255)                                                                                                           ccGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGa                <  1:1007251/137‑1 (MQ=255)                                                                                                           ccGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGa                >  2:82064/1‑137 (MQ=255)                                                                                                           ccGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGa                <  1:82064/137‑1 (MQ=255)                                                                                                           ccGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGAtt              >  2:555962/1‑139 (MQ=255)                                                                                                           ccGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGAtt              >  1:639317/1‑139 (MQ=255)                                                                                                           ccGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGAtt              >  2:152554/1‑139 (MQ=255)                                                                                                                       aCGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGTTa  <  2:788428/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                CGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGTTA  >  NC_000913/37774‑38030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑