Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 414,875 A→G R293R (CGT→CGC sbcC ← exonuclease, dsDNA, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913414,8750AG100.0% 110.8 / NA 32R293R (CGT→CGCsbcCexonuclease, dsDNA, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/14);  total (18/14)

CTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCG  >  NC_000913/414746‑415005
                                                                                                                                 |                                                                                                                                  
cTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGAttt                                                                                                                           >  1:66773/1‑139 (MQ=255)
        cgTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg                                                                                                                   <  2:284923/139‑1 (MQ=255)
         gtggtgGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGt                                                                                                                  <  2:149168/139‑1 (MQ=255)
                               aGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCa                                                                                            >  1:440105/1‑139 (MQ=255)
                                              tGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCtt                                                                             >  1:642456/1‑139 (MQ=255)
                                                   gCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGAt                                                                                                                             <  1:861362/86‑1 (MQ=255)
                                                   gCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGAt                                                                                                                             >  2:861362/1‑86 (MQ=255)
                                                        tATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTttcttcttc                                                                   >  1:579573/1‑139 (MQ=255)
                                                        tATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTttcttcttc                                                                   >  2:533969/1‑139 (MQ=255)
                                                          tttACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCgg                                                                 >  2:744952/1‑139 (MQ=255)
                                                          tttACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGCTGAGGTTGCGCTTTTTCTTCTTCgg                                                                 >  1:1056429/1‑139 (MQ=255)
                                                                 cttcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCt                                                               <  1:146292/134‑1 (MQ=255)
                                                                 cttcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCt                                                               >  2:146292/1‑134 (MQ=255)
                                                                  ttcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGcc                                                         <  1:533969/139‑1 (MQ=255)
                                                                           ctgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg                                                                                                                   <  1:1246986/72‑1 (MQ=255)
                                                                           ctgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg                                                                                                                   >  2:1246986/1‑72 (MQ=255)
                                                                            tgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCttttt                                                                          >  2:487055/1‑112 (MQ=255)
                                                                            tgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCttttt                                                                          <  1:487055/112‑1 (MQ=255)
                                                                              gCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg                                                                                                                   <  2:13085/69‑1 (MQ=255)
                                                                              gCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg                                                                                                                   >  1:13085/1‑69 (MQ=255)
                                                                                     tGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGAc                                      >  1:645053/1‑139 (MQ=255)
                                                                                        gcCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTg                                                                                  <  1:72473/92‑1 (MQ=255)
                                                                                        gcCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTg                                                                                  >  2:72473/1‑92 (MQ=255)
                                                                                             cgccgcGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCggctg                              >  2:28740/1‑139 (MQ=255)
                                                                                                            tGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg                                                                                                                   <  1:151212/39‑1 (MQ=38)
                                                                                                            tGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg                                                                                                                   >  2:151212/1‑39 (MQ=38)
                                                                                                            tGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCTTCTTGCTGCAAt               <  1:275460/139‑1 (MQ=255)
                                                                                                                   cGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAAc                                                            >  1:623364/1‑87 (MQ=255)
                                                                                                                   cGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAAc                                                            <  2:623364/87‑1 (MQ=255)
                                                                                                                         cAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGAcgcg  <  2:642456/139‑1 (MQ=255)
                                                                                                                            tgtgGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGcgcc                                                                                                <  2:341562/42‑1 (MQ=255)
                                                                                                                            tgtgGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGcgcc                                                                                                >  1:341562/1‑42 (MQ=255)
                                                                                                                                 |                                                                                                                                  
CTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCG  >  NC_000913/414746‑415005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: