Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 443,604 C→T S13N (AGT→AAT) 
*304* (TAG→TAA
yajL ←
panE ←
oxidative‑stress‑resistance chaperone
2‑dehydropantoate reductase, NADPH‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913443,6040CT100.0% 63.1 / NA 21S13N (AGT→AAT) 
*304* (TAG→TAA
yajL
panE
oxidative‑stress‑resistance chaperone
2‑dehydropantoate reductase, NADPH‑specific
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/14);  total (7/14)

GCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTA  >  NC_000913/443495‑443692
                                                                                                             |                                                                                        
gcgcgAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGAt                                                             <  2:929563/139‑1 (MQ=255)
 cgcgAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg                                                            <  1:534817/139‑1 (MQ=255)
   cgAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATgcg                                                          <  1:1162287/139‑1 (MQ=255)
    gAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATgcgc                                                         <  1:470840/139‑1 (MQ=255)
            aaTCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTc                                                 <  2:1147447/139‑1 (MQ=255)
             aTCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAAc                                                                       >  1:124790/1‑116 (MQ=255)
             aTCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAAc                                                                       <  2:124790/116‑1 (MQ=255)
             aTCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCa                                                <  1:551026/139‑1 (MQ=255)
                               cTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTCCAGTCTCTTCATTACCAGGGGCGAGGCAAAc                                                                       <  1:531408/98‑1 (MQ=255)
                               cTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTCCAGTCTCTTCATTACCAGGGGCGAGGCAAAc                                                                       >  2:531408/1‑98 (MQ=255)
                                            cAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGc                 <  2:439458/139‑1 (MQ=255)
                                            cAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGc                 <  2:226113/139‑1 (MQ=255)
                                                     cTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCAct                                              >  1:18440/1‑101 (MQ=255)
                                                     cTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCAct                                              <  2:18440/101‑1 (MQ=255)
                                                           tACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTa  >  1:1092737/1‑139 (MQ=255)
                                                                                        gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTAc                               >  1:868811/1‑81 (MQ=255)
                                                                                        gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTAc                               <  2:868811/81‑1 (MQ=255)
                                                                                           aCGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATgcg                                                          <  1:906025/51‑1 (MQ=255)
                                                                                           aCGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATgcg                                                          >  2:906025/1‑51 (MQ=255)
                                                                                                         ttCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCat                                                     >  2:269623/1‑42 (MQ=255)
                                                                                                         ttCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCat                                                     <  1:269623/42‑1 (MQ=255)
                                                                                                             |                                                                                        
GCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTA  >  NC_000913/443495‑443692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: