Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	444,641	C→T	intergenic (‑126/‑42)	panE ← / → yajQ	2‑dehydropantoate reductase, NADPH‑specific/phage Phi6 host factor, ATP/GTP binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	444,641	0	C	T	100.0%	156.2 / NA	49	intergenic (‑126/‑42)	panE/yajQ	2‑dehydropantoate reductase, NADPH‑specific/phage Phi6 host factor, ATP/GTP binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/19);  total (30/19)

TAATTTTCATTGTTCCTCCTCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGCGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGTCCCG  >  NC_000913/444506‑444768                                                                                                                                        |                                                                                                                                tAATTTTCATTGTTCCTCCTCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTggg                                                                                                                              >  1:937370/1‑139 (MQ=255) tAATTTTCATTGTTCCTCCTCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTggg                                                                                                                              >  1:33637/1‑139 (MQ=255)                          gCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGaa                                                                                                     >  2:632535/1‑139 (MQ=255)                                          ttCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTgg                                                                                                                               <  2:265688/97‑1 (MQ=255)                                          ttCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTgg                                                                                                                               >  1:265688/1‑97 (MQ=255)                                            cAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGaa                                                                                                     <  2:432863/121‑1 (MQ=255)                                            cAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGaa                                                                                                     >  1:432863/1‑121 (MQ=255)                                                        cccTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGatat                                                                       <  1:442685/139‑1 (MQ=255)                                                        cccTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGatat                                                                       <  1:1009974/139‑1 (MQ=255)                                                         ccTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGTATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGTATCAATAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATAtt                                                                      <  1:865915/139‑1 (MQ=255)                                                                   gCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAg                                                            >  2:630014/1‑139 (MQ=255)                                                                    cATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCa                                                                                                                >  1:186301/1‑86 (MQ=255)                                                                    cATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCa                                                                                                                <  2:186301/86‑1 (MQ=255)                                                                    cATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGt                                                           >  1:878818/1‑139 (MQ=255)                                                                       gCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGATTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGa                                                        >  1:1220834/1‑139 (MQ=255)                                                                         tGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATc                                                      >  2:609825/1‑139 (MQ=255)                                                                            tGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGc                                                                                    >  2:1215136/1‑106 (MQ=255)                                                                            tGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGc                                                                                    <  1:1215136/106‑1 (MQ=255)                                                                                   aTGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGa                                                                                                      <  2:784180/81‑1 (MQ=255)                                                                                   aTGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGa                                                                                                      >  1:784180/1‑81 (MQ=255)                                                                                    tGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCa                                           >  1:848974/1‑139 (MQ=255)                                                                                     gCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTgg                                                                                                                               >  1:182768/1‑54 (MQ=255)                                                                                     gCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCAc                                          >  2:988762/1‑139 (MQ=255)                                                                                           ttAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCa                                                                                                             <  2:674247/66‑1 (MQ=255)                                                                                           ttAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCa                                                                                                             >  1:674247/1‑66 (MQ=255)                                                                                           ttAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAAcg                                    >  1:543403/1‑139 (MQ=255)                                                                                              aGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGtt                                                          <  1:906111/114‑1 (MQ=255)                                                                                              aGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGtt                                                          >  2:906111/1‑114 (MQ=255)                                                                                               gggATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGt                                <  1:988762/139‑1 (MQ=255)                                                                                                   tACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTgg                                                                                                                               >  2:777396/1‑40 (MQ=255)                                                                                                   tACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTgg                                                                                                                               >  2:822501/1‑40 (MQ=255)                                                                                                     cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAggg                                                                                                  <  2:982963/67‑1 (MQ=255)                                                                                                     cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAggg                                                                                                  >  1:982963/1‑67 (MQ=255)                                                                                                     cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATAtt                                                                      >  2:773198/1‑95 (MQ=255)                                                                                                     cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATAtt                                                                      <  1:773198/95‑1 (MQ=255)                                                                                                                aGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCcgcg               >  2:871861/1‑139 (MQ=255)                                                                                                                  gACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGaaa                                                                                          <  2:716627/62‑1 (MQ=255)                                                                                                                  gACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGaaa                                                                                          >  1:716627/1‑62 (MQ=255)                                                                                                                   aCTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAg            <  2:848974/139‑1 (MQ=255)                                                                                                                     tGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTg          <  2:937370/139‑1 (MQ=255)                                                                                                                       tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGtt                                                          >  2:1016149/1‑89 (MQ=255)                                                                                                                       tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGtt                                                          <  1:1016149/89‑1 (MQ=255)                                                                                                                       tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGa        >  2:465705/1‑139 (MQ=255)                                                                                                                       tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGa        >  1:178650/1‑139 (MQ=255)                                                                                                                       tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGa        >  1:1273408/1‑139 (MQ=255)                                                                                                                          gTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGTc     <  2:1273408/139‑1 (MQ=255)                                                                                                                             aTTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGATCCGCGAAGTGGAGTCCCg  >  1:872074/1‑139 (MQ=255)                                                                                                                                     ttGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCa                                                                                  >  2:604086/1‑51 (MQ=255)                                                                                                                                     ttGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCa                                                                                  <  1:604086/51‑1 (MQ=255)                                                                                                                                        |                                                                                                                                TAATTTTCATTGTTCCTCCTCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGCGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGTCCCG  >  NC_000913/444506‑444768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑