Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	475,667	T→C	S97P (TCA→CCA)	ybaY →	outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	475,667	0	T	C	100.0%	174.4 / NA	49	S97P (TCA→CCA)	ybaY	outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/25);  total (24/25)

TACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAAC  >  NC_000913/475534‑475798                                                                                                                                      |                                                                                                                                    tACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGccacca                                                                                                                                >  1:1048129/1‑139 (MQ=255)   ccGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCAtt                                                                                                                              <  1:885599/139‑1 (MQ=255)      tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAg                                                                                                                           >  1:162776/1‑139 (MQ=255)              cGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTc                                                                                                                   <  1:834938/139‑1 (MQ=255)                                             tgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGat                                                                                    <  2:1097734/139‑2 (MQ=255)                                             tgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGaa                                                                                    >  2:473735/1‑139 (MQ=255)                                                tgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgc                                                                                 <  2:82491/139‑1 (MQ=255)                                                  aCCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                               >  2:1173825/1‑139 (MQ=255)                                                  aCCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                               <  1:473735/139‑1 (MQ=255)                                                  aCCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgc                                                                                 <  2:611658/137‑1 (MQ=255)                                                  aCCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgc                                                                                 >  1:611658/1‑137 (MQ=255)                                                       gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGccacca                                                                                                                                >  2:733168/1‑85 (MQ=255)                                                       gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGccacca                                                                                                                                <  1:733168/85‑1 (MQ=255)                                                       gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGtt                                                                                                                    >  2:330740/1‑97 (MQ=255)                                                       gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGtt                                                                                                                    <  1:330740/97‑1 (MQ=255)                                                       gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTAtt                                                                          >  1:1123037/1‑139 (MQ=255)                                                       gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTAtt                                                                          >  1:1131999/1‑139 (MQ=255)                                                       gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCACAGAAAGCGGTGCGTACTGAAGGTAAACAGccacca                                                                                                                                <  2:83695/85‑1 (MQ=255)                                                       gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCACAGAAAGCGGTGCGTACTGAAGGTAAACAGccacca                                                                                                                                >  1:83695/1‑85 (MQ=255)                                                              tCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGa                                                                   >  1:729795/1‑139 (MQ=255)                                                              tCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGa                                                                   >  2:112390/1‑139 (MQ=255)                                                                  aCGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTgc                                                               >  1:672725/1‑139 (MQ=255)                                                                      gtcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                               >  1:740942/1‑119 (MQ=255)                                                                      gtcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCCTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGGTGTTCAGCCGAAcgcgc                                                                               <  2:740942/119‑1 (MQ=255)                                                                       tcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAg                                                                                                 >  2:957538/1‑100 (MQ=255)                                                                       tcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAg                                                                                                 <  1:957538/100‑1 (MQ=255)                                                                          ttAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTa                                                       <  2:1246773/139‑1 (MQ=255)                                                                            aGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTAcc                                                     <  2:1162430/139‑1 (MQ=255)                                                                                  tGCACCGTCAAGAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAAt                                               >  1:342130/1‑139 (MQ=255)                                                                                  tGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTGAGCCGCAACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGCTTGCGCGTATTCTGCTGAGTGGGGCGATTACCGTGagt                                               >  2:101462/1‑137 (MQ=255)                                                                                  tGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTCTGTTCTGTCATTTAACCCGGCAGATGTTCAGGCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAAt                                               <  1:794475/139‑1 (MQ=255)                                                                                   gCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATg                                              <  1:1173825/139‑1 (MQ=255)                                                                                       cGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACaa                                          <  1:421811/139‑1 (MQ=255)                                                                                        gTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACaaa                                         <  2:162776/139‑1 (MQ=255)                                                                                        gTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACaaa                                         >  2:238906/1‑139 (MQ=255)                                                                                         tCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTg                                                                                                                 >  1:468199/1‑66 (MQ=255)                                                                                         tCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTg                                                                                                                 <  2:468199/66‑1 (MQ=255)                                                                                                  cTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTg                                                                                                                      <  2:982325/52‑1 (MQ=255)                                                                                                  cTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTg                                                                                                                      >  1:982325/1‑52 (MQ=255)                                                                                                   tGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTgc                                                               >  2:315760/1‑106 (MQ=255)                                                                                                   tGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTgc                                                               <  1:315760/106‑1 (MQ=255)                                                                                                   tGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTg                                                  <  2:2164/119‑1 (MQ=255)                                                                                                   tGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTg                                                  >  1:2164/1‑119 (MQ=255)                                                                                                    ggCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTa                                                       <  2:551870/113‑1 (MQ=255)                                                                                                    ggCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTa                                                       >  1:551870/1‑113 (MQ=255)                                                                                                                ggTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGtt                 <  2:729795/139‑1 (MQ=255)                                                                                                                 gTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTgc                                                               <  2:544064/92‑1 (MQ=255)                                                                                                                 gTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTgc                                                               >  1:544064/1‑92 (MQ=255)                                                                                                                     gTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGc                                                                                                   <  2:474031/52‑1 (MQ=255)                                                                                                                     gTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGc                                                                                                   >  1:474031/1‑52 (MQ=255)                                                                                                                     gTACTGAAGGTAAACAGCCACCATTCAGCTTTGTCCTGTCATTTAACCCGGGAGATGTTCAGCCGAACGCGCGTATTCTGTTTAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGcc            <  2:307180/139‑1 (MQ=255)                                                                                                                           aaGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGAt      >  2:1008589/1‑139 (MQ=255)                                                                                                                               tAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAAc  <  2:672725/139‑1 (MQ=255)                                                                                                                                aaaCAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTg                                      >  2:807697/1‑102 (MQ=255)                                                                                                                                aaaCAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTg                                      <  1:807697/102‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    TACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAAC  >  NC_000913/475534‑475798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑