Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 281,662 Δ1 bp coding (322/1155 nt) yagA ← CP4‑6 prophage, putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913281,6600G.100.0% 53.6 / NA 14coding (324/1155 nt)yagACP4‑6 prophage, putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (7/7);  total (7/7)

GCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCG  >  NC_000913/281565‑281770
                                                                                               |                                                                                                              
gcgTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCg                                                                    <  2:1005143/139‑1 (MQ=255)
                   ccGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGa                                                 <  1:1321373/139‑1 (MQ=255)
                       ggCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGt                                             >  1:812237/1‑139 (MQ=255)
                                             cccGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCtt                                                                       >  1:918942/1‑91 (MQ=255)
                                             cccGGCAGCAGGCCAAGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCtt                                                                       <  2:918942/91‑1 (MQ=255)
                                                        gCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATgtcgt            >  1:1076956/1‑139 (MQ=255)
                                                          cATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCa                                           <  1:1124392/106‑1 (MQ=255)
                                                          cATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCa                                           >  2:1124392/1‑106 (MQ=255)
                                                                gggCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAg    <  2:1076956/139‑1 (MQ=255)
                                                                  gCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAg    >  1:460596/1‑137 (MQ=255)
                                                                  gCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAg    <  2:460596/137‑1 (MQ=255)
                                                                  gCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCg  >  2:1067457/1‑139 (MQ=255)
                                                                            tATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCtt                                                                       <  1:1202663/60‑1 (MQ=255)
                                                                            tATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCtt                                                                       >  2:1202663/1‑60 (MQ=255)
                                                                                               |                                                                                                              
GCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCG  >  NC_000913/281565‑281770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: