Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,860,426 Δ1 bp intergenic (+10/+41) pphB → / ← ygbI serine/threonine‑specific protein phosphatase 2/DeoR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,860,4250T.100.0% 252.5 / NA 60intergenic (+9/+42)pphB/ygbIserine/threonine‑specific protein phosphatase 2/DeoR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (31/29);  total (31/29)

TGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTA  >  NC_000913/2860299‑2860548
                                                                                                                              |                                                                                                                           
tGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGc                                                                                                                <  1:461552/139‑1 (MQ=255)
     attTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAg                                                                                                           >  2:409893/1‑139 (MQ=255)
            atTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                     <  1:284648/138‑1 (MQ=255)
            atTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                     >  2:284648/1‑138 (MQ=255)
                ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                                <  1:409893/139‑1 (MQ=255)
                           gTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGct                                                                                                                             >  2:294244/1‑100 (MQ=255)
                           gTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGct                                                                                                                             <  1:294244/100‑1 (MQ=255)
                           gTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGcc                                                                                     >  1:382530/1‑139 (MQ=255)
                            tttGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCtgtg                                                                                                                    <  1:132886/107‑1 (MQ=255)
                            tttGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCtgtg                                                                                                                    >  2:132886/1‑107 (MQ=255)
                              tGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGt                                                                                                          <  1:788752/115‑1 (MQ=255)
                              tGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGt                                                                                                          >  2:788752/1‑115 (MQ=255)
                                aTAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                     <  2:367725/118‑1 (MQ=255)
                                aTAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                     >  1:367725/1‑118 (MQ=255)
                                   aCATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCa                                                                             <  2:382530/139‑1 (MQ=255)
                                      ttCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGc                                                                                                                <  1:429436/101‑1 (MQ=255)
                                      ttCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGc                                                                                                                >  2:429436/1‑101 (MQ=255)
                                         aGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                     >  1:364585/1‑109 (MQ=255)
                                         aGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAccc                                                                                                     <  2:364585/109‑1 (MQ=255)
                                              ttCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCa                                                                                         <  2:525083/116‑1 (MQ=255)
                                              ttCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCa                                                                                         >  1:525083/1‑116 (MQ=255)
                                              ttCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTAc                                                                  >  2:619284/1‑139 (MQ=255)
                                               tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                                   >  1:37392/1‑137 (MQ=255)
                                               tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                                   <  2:37392/137‑1 (MQ=255)
                                               tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTAcc                                                                 >  1:958356/1‑139 (MQ=255)
                                                cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCa                                                                >  1:1313965/1‑139 (MQ=255)
                                                   tAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCa                                                             <  2:1313965/139‑1 (MQ=255)
                                                   tAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCa                                                             <  2:958356/139‑1 (MQ=255)
                                                          aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAagagag                                                      >  1:357548/1‑139 (MQ=255)
                                                            ttATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGAt                                                    <  1:619284/139‑1 (MQ=255)
                                                               tatTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCt                                                 >  2:468383/1‑139 (MQ=255)
                                                                    aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTaa                                                                                                        <  1:515574/79‑1 (MQ=255)
                                                                    aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTaa                                                                                                        >  2:515574/1‑79 (MQ=255)
                                                                    aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAcc                                                                                                      >  1:195818/1‑81 (MQ=255)
                                                                    aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAAcc                                                                                                      <  2:195818/81‑1 (MQ=255)
                                                                    aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCatta                                                                                 >  1:1361959/1‑102 (MQ=255)
                                                                    aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCatta                                                                                 <  2:1361959/102‑1 (MQ=255)
                                                                    aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                            >  2:374219/1‑139 (MQ=255)
                                                                        cGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCa                                        >  1:1378172/1‑139 (MQ=255)
                                                                         ggATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAgg                                                                                       <  1:471116/91‑1 (MQ=255)
                                                                         ggATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAgg                                                                                       >  2:471116/1‑91 (MQ=255)
                                                                            tCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcgc                                    <  1:374219/139‑1 (MQ=255)
                                                                              gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCtg                                                                                                                      >  1:321747/1‑55 (MQ=255)
                                                                              gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCtg                                                                                                                      <  2:321747/55‑1 (MQ=255)
                                                                              gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcgcg                                   <  2:429191/138‑1 (MQ=255)
                                                                              gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcgcg                                   >  1:429191/1‑138 (MQ=255)
                                                                               ccGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAagaga                                                       >  1:69920/1‑117 (MQ=255)
                                                                               ccGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAagaga                                                       <  2:69920/117‑1 (MQ=255)
                                                                                cGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAgg                                                                                       <  1:1165890/84‑1 (MQ=255)
                                                                                cGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAgg                                                                                       >  2:1165890/1‑84 (MQ=255)
                                                                                cGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGa                                <  1:468383/139‑1 (MQ=255)
                                                                                       cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCa                                                                                         <  2:1392686/75‑1 (MQ=255)
                                                                                       cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCa                                                                                         >  1:1392686/1‑75 (MQ=255)
                                                                                       cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCAc                         >  1:1163425/1‑139 (MQ=255)
                                                                                         ggcggcTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGc                                                                     >  2:137904/1‑93 (MQ=255)
                                                                                         ggcggcTGTCATTTTATAAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGc                                                                     <  1:137904/93‑1 (MQ=255)
                                                                                                       tataAAATAAAGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtc         <  2:1163425/139‑1 (MQ=255)
                                                                                                           aaataaaGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCt     >  1:515229/1‑139 (MQ=255)
                                                                                                           aaataaaGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCt     >  1:379058/1‑139 (MQ=255)
                                                                                                              taaaGTAATCTCATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGta  <  2:379058/139‑1 (MQ=255)
                                                                                                                      tctcATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcgcgc                                  <  1:870388/99‑1 (MQ=255)
                                                                                                                      tctcATGCTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcgcgc                                  >  2:870388/1‑99 (MQ=255)
                                                                                                                              |                                                                                                                           
TGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTA  >  NC_000913/2860299‑2860548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: