Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,742,599 Δ1 bp intergenic (‑67/‑134) yiaJ ← / → yiaK transcriptional repressor for the yiaKLMNO‑lyxK‑sgbHUE operon/2,3‑diketo‑L‑gulonate reductase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,742,5980C.100.0% 101.0 / NA 25intergenic (‑66/‑135)yiaJ/yiaKtranscriptional repressor for the yiaKLMNO‑lyxK‑sgbHUE operon/2,3‑diketo‑L‑gulonate reductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (13/12);  total (13/12)

GGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCAGATTGCCTTGT  >  NC_000913/3742463‑3742645
                                                                                                                                       |                                               
ggCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAAcaat                                              <  2:1414674/139‑4 (MQ=255)
           ggACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATg                                  >  1:1110182/1‑139 (MQ=255)
           ggACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATg                                  >  2:619171/1‑139 (MQ=255)
                       tCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTc                                <  1:1383565/129‑1 (MQ=255)
                       tCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTc                                >  2:1383565/1‑129 (MQ=255)
                          tGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATtcat                   <  2:311322/139‑1 (MQ=255)
                                 tctcGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGaaa                        >  1:1328208/1‑127 (MQ=255)
                                 tctcGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGaaa                        <  2:1328208/127‑1 (MQ=255)
                                    cGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt         <  2:1231302/139‑6 (MQ=255)
                                           tttttttCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgttgacaatt  >  1:1196146/1‑127 (MQ=255)
                                             tttttCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATtcatca                 <  2:813142/122‑1 (MQ=255)
                                             tttttCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATtcatca                 >  1:813142/1‑122 (MQ=255)
                                                                 cccATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCttt                             >  2:501686/1‑90 (MQ=255)
                                                                 cccATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCttt                             <  1:501686/90‑1 (MQ=255)
                                                                               tCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatc              <  1:42143/91‑1 (MQ=255)
                                                                               tCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatc              >  2:42143/1‑91 (MQ=255)
                                                                                         aGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATtcatca                 <  2:678465/78‑1 (MQ=255)
                                                                                         aGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATtcatca                 >  1:678465/1‑78 (MQ=255)
                                                                                          gcgcAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATtcat                   >  2:1378017/1‑75 (MQ=255)
                                                                                          gcgcAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATtcat                   <  1:1378017/75‑1 (MQ=255)
                                                                                                      ccGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTAt                                   <  1:1376977/47‑1 (MQ=255)
                                                                                                      ccGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTAt                                   >  2:1376977/1‑47 (MQ=255)
                                                                                                            ttCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt         <  1:1039585/67‑6 (MQ=255)
                                                                                                            ttCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt         >  2:1039585/1‑62 (MQ=255)
                                                                                                                cGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt         <  1:539577/63‑6 (MQ=255)
                                                                                                                cGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt         >  2:539577/1‑58 (MQ=255)
                                                                                                                                       |                                               
GGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCAGATTGCCTTGT  >  NC_000913/3742463‑3742645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: