Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,399,985 (C)7→8 coding (252/516 nt) ogt ← O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,399,9781.C100.0% 134.1 / NA 38T87A (ACG→GCG) ogtO‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (19/19);  total (19/19)

GGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC  >  NC_000913/1399842‑1400111
                                                                                                                                         |                                                                                                                                     
ggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc                                                                                                                                     <  2:1217210/139‑1 (MQ=255)
  aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc                                                                                                                                   <  2:178067/139‑1 (MQ=255)
   ttCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccccc                                                                                                                                  >  2:509611/1‑139 (MQ=255)
    tCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccccc                                                                                                                                 >  2:589989/1‑139 (MQ=255)
       aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGCCTTCGCGCTGAAATGGCGT‑ccccccccg                                                                                                                              <  1:208004/139‑3 (MQ=255)
       aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccccccccg                                                                                                                              <  1:1127094/139‑3 (MQ=255)
       aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccccccccg                                                                                                                              <  2:257728/139‑3 (MQ=255)
                 cGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTggg                                                                                                                     <  2:599352/139‑1 (MQ=255)
                 cGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTggg                                                                                                                     <  1:1041048/139‑1 (MQ=255)
                 cGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTggg                                                                                                                     >  1:599352/1‑139 (MQ=255)
                  gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGa                                                                                                                    <  1:589989/139‑1 (MQ=255)
                    aCCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAg                                                                                                                  <  2:1289637/139‑1 (MQ=255)
                              cgcgccgcgcCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCaata                                                                                                        >  2:902504/1‑139 (MQ=255)
                              cgcgccgcgcCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCaata                                                                                                        >  2:1287062/1‑139 (MQ=255)
                                                cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa                                                                                      >  1:40661/1‑139 (MQ=255)
                                                cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa                                                                                      >  2:376683/1‑139 (MQ=255)
                                                          cAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGTGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTAc                                                                                           <  2:112656/124‑1 (MQ=255)
                                                          cAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTAc                                                                                           >  1:112656/1‑124 (MQ=255)
                                                          cAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCAc                                                                            >  1:479572/1‑139 (MQ=255)
                                                          cAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCAc                                                                            >  1:144909/1‑139 (MQ=255)
                                                                 ttGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt                                                                     <  2:479572/139‑1 (MQ=255)
                                                                 ttGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt                                                                     <  2:144909/139‑1 (MQ=255)
                                                                 ttGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt                                                                     <  1:469371/139‑1 (MQ=255)
                                                                     ccGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCg                                                                 >  1:930773/1‑139 (MQ=255)
                                                                           tGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTggg                                                                                                                     <  2:992759/81‑1 (MQ=255)
                                                                           tGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTggg                                                                                                                     >  1:992759/1‑81 (MQ=255)
                                                                                     gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGa                                                                                                                    >  2:1331164/1‑72 (MQ=255)
                                                                                     gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGa                                                                                                                    <  1:1331164/72‑1 (MQ=255)
                                                                                     gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccccccccgtag                                                                                                                           >  1:694402/1‑59 (MQ=255)
                                                                                     gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccccccccgtag                                                                                                                           <  2:694402/64‑6 (MQ=255)
                                                                                      cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAtt                                                >  2:785795/1‑139 (MQ=255)
                                                                                          cAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATtggtg                                            >  2:70732/1‑139 (MQ=255)
                                                                                                    tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCaa                                                                                     >  2:122005/1‑88 (MQ=255)
                                                                                                    tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCaa                                                                                     <  1:122005/88‑1 (MQ=255)
                                                                                                       gTAGTGTTTTCCAGACATCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCaa                                                                                                          <  2:1392650/64‑1 (MQ=255)
                                                                                                       gTAGTGTTTTCCAGACATCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCaa                                                                                                          >  1:1392650/1‑64 (MQ=255)
                                                                                                        tAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCa                                         <  2:1354934/128‑1 (MQ=255)
                                                                                                        tAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCa                                         >  1:1354934/1‑128 (MQ=255)
                                                                                                                   aGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCcttctt                   <  2:930773/139‑1 (MQ=255)
                                                                                                                    gACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCttt                  <  1:785795/139‑1 (MQ=255)
                                                                                                                     aCTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCcttct                    >  1:1190440/1‑136 (MQ=255)
                                                                                                                     aCTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCcttct                    <  2:1190440/136‑1 (MQ=255)
                                                                                                                       cttgagcagAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaa                                                            <  2:519609/86‑1 (MQ=255)
                                                                                                                       cttgagcagAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaa                                                            >  1:519609/9‑94 (MQ=255)
                                                                                                                         cgcgCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCaat                                                                                                         <  1:94497/47‑1 (MQ=255)
                                                                                                                         cgcgCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCaat                                                                                                         >  2:94497/1‑47 (MQ=255)
                                                                                                                                    ggCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTcc  <  1:70732/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                     
GGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC  >  NC_000913/1399842‑1400111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑