| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,728,118 | (C)8→9 | coding (1254/1455 nt) | xylB ← | xylulokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,728,110 | 1 | . | C | 95.0% | 134.0 / 0.0 | 40 | coding (1262/1455 nt) | xylB | xylulokinase |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (17/21); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
CTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCG > NC_000913/3727974‑3728223 | cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑cc < 1:654913/139‑1 (MQ=255) tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACgg > 2:887128/1‑139 (MQ=255) tCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑CCCCCCCCGTACGGTAATCGAgctgct < 1:439182/139‑1 (MQ=255) cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGATCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAAC‑‑CCCCCCCCGTACGGTAATCGAgctgct < 1:1051200/139‑1 (MQ=255) cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAgctgct > 2:24954/1‑139 (MQ=255) cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAgctgct < 2:923621/139‑1 (MQ=255) cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAgctgct < 1:485654/139‑1 (MQ=255) ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑cccccccccg > 1:962670/1‑117 (MQ=255) ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑cccccccccg < 2:962670/119‑3 (MQ=255) tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATCCCCCCCCCCCTACGGTAATCGAGCTGCCGACCGcc > 2:140969/1‑138 (MQ=255) cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATc > 1:475963/1‑139 (MQ=255) tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc > 1:604164/1‑139 (MQ=255) gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGCCCGCTGATATCCGCCAGCATCt < 2:249745/139‑1 (MQ=255) tAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGA‑TCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa < 1:445860/139‑1 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa < 2:657216/139‑1 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑cccccccccgtac > 1:338689/1‑93 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑cccccccccgtac < 2:338689/98‑6 (MQ=255) tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc < 1:182854/139‑1 (MQ=255) tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc < 2:971751/139‑1 (MQ=255) cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATc > 1:989293/1‑100 (MQ=255) cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATc < 2:989293/100‑1 (MQ=255) cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAgctg > 2:1113701/1‑106 (MQ=255) cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAgctg < 1:1113701/106‑1 (MQ=255) cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGATCGCTGATATCCGCCAGCATCTGACGCCa < 1:887128/139‑1 (MQ=255) aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcg < 1:24954/139‑1 (MQ=255) gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc > 2:917852/1‑139 (MQ=255) ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg > 1:148122/1‑105 (MQ=255) ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg < 2:148122/105‑1 (MQ=255) tGGATTCGCTGCGATCTGCGCCAGCCTTGCTGCGGCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt < 1:917852/139‑1 (MQ=255) tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt < 2:604164/139‑1 (MQ=255) tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt < 2:475963/139‑1 (MQ=255) gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa < 1:632104/100‑1 (MQ=255) gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa > 2:632104/1‑100 (MQ=255) gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa < 1:951302/105‑1 (MQ=255) gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa > 2:951302/1‑105 (MQ=255) gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCCGTACTCCCTACCCGCCCCGCCCCCAATCAACGTa > 1:339433/1‑139 (MQ=255) ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGAc > 2:617995/1‑76 (MQ=255) ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGAc < 1:617995/76‑1 (MQ=255) cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg < 1:1280576/139‑1 (MQ=255) gcgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGATCTGCTGACCGCTGATATCCGCCAGCACCTGACGCCAGTACCCACTACCCGCCCCGCCCCCCAACAACGTAAAACTTTCCg > 2:1280576/1‑139 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc < 2:1300922/86‑1 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc > 1:1300922/1‑86 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑cccccccccgta > 2:377627/1‑37 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑cccccccccgta < 1:377627/41‑5 (MQ=255) gctgcGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg > 1:593526/1‑139 (MQ=255) gcCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGccccc > 1:236479/1‑103 (MQ=255) gcCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGccccc < 2:236479/103‑1 (MQ=255) | CTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCG > NC_000913/3727974‑3728223 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |