Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	490,285	C→T	*176* (TAG→TAA)	priC ←	primosomal replication protein N''

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	490,285	0	C	T	100.0%	115.1 / NA	37	*176* (TAG→TAA)	priC	primosomal replication protein N''
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/18);  total (19/18)

TAATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAG  >  NC_000913/490150‑490416                                                                                                                                        |                                                                                                                                    tAATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAg                                                                                                                                  <  2:767112/139‑1 (MQ=255)                  atCCAGCGCGCGCAGCACGGTACTGGC‑GGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAAc                                                                                                                >  1:1201277/1‑139 (MQ=255)                      aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTaa                                                                                                                          <  1:1069070/126‑1 (MQ=255)                      aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTaa                                                                                                                          >  2:1069070/1‑126 (MQ=255)                      aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTCAGCGGGTTAAACGCGCTAACCTg                                                                                                             >  1:846158/1‑139 (MQ=255)                           gcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTaaa                                                                                                                         >  2:394293/1‑122 (MQ=255)                           gcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTaaa                                                                                                                         <  1:394293/122‑1 (MQ=255)                            cgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGtttt                                                                                                         <  2:468124/137‑1 (MQ=255)                            cgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGtttt                                                                                                         >  1:468124/1‑137 (MQ=255)                             gcagcaCGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGa                                                                                                      >  1:159326/1‑139 (MQ=255)                                           ggcgggATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCa                                                                                        <  2:1201277/136‑1 (MQ=255)                                                  tCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTaa                                                                                                                          >  1:1201772/1‑98 (MQ=255)                                                  tCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTaa                                                                                                                          <  2:1201772/98‑1 (MQ=255)                                                               cagcagCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcg                                                                    <  2:159326/139‑1 (MQ=255)                                                                agcagcACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcgc                                                                   >  2:348719/1‑139 (MQ=255)                                                                     cacaATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAg                                                                                                                                  <  1:232020/71‑1 (MQ=255)                                                                     cacaATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAg                                                                                                                                  >  2:232020/1‑71 (MQ=255)                                                                     cacaATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGtttt                                                                                                         >  1:1152515/1‑96 (MQ=255)                                                                     cacaATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGtttt                                                                                                         <  2:1152515/96‑1 (MQ=255)                                                                     cacaATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTc                                                              >  2:519056/1‑139 (MQ=255)                                                                      acaATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCa                                                             <  1:519056/139‑1 (MQ=255)                                                                               tCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCg                                                                                                       >  1:449753/1‑88 (MQ=255)                                                                               tCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCg                                                                                                       <  2:449753/88‑1 (MQ=255)                                                                                    aGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcgc                                                                   >  1:649927/1‑119 (MQ=255)                                                                                    aGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcgc                                                                   <  2:649927/119‑1 (MQ=255)                                                                                                  cGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGttt                                 <  2:67026/139‑1 (MQ=255)                                                                                                    tCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAg                                                                               >  1:936830/1‑91 (MQ=255)                                                                                                    tCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAg                                                                               <  2:936830/91‑1 (MQ=255)                                                                                                      aGGGGCATTTTCCAGTGACATATTCTCTCCATTGTCAGCGGGTTAAACGCGCTAACCAGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTg                             <  2:846158/139‑1 (MQ=255)                                                                                                           cATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCa                        <  1:348719/139‑1 (MQ=255)                                                                                                                               tctcCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGAtttt                                                                                                  <  2:1342394/45‑1 (MQ=255)                                                                                                                               tctcCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGAtttt                                                                                                  >  1:1342394/1‑45 (MQ=255)                                                                                                                                 tcCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGAt                  >  1:328773/1‑123 (MQ=255)                                                                                                                                 tcCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGAt                  <  2:328773/123‑1 (MQ=255)                                                                                                                                 tcCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAg  >  1:231300/1‑139 (MQ=255)                                                                                                                                 tcCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAg  >  2:511353/1‑139 (MQ=255)                                                                                                                                 tcCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAg  >  2:1313625/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                    TAATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAG  >  NC_000913/490150‑490416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑