Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	571,444	G→A	*184* (TAG→TAA)	ybcL →	inactive polymorphonuclear leukocyte migration suppressor, DLP12 prophage, UPF0098 family secreted protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	571,444	0	G	A	100.0%	272.1 / NA	84	*184* (TAG→TAA)	ybcL	inactive polymorphonuclear leukocyte migration suppressor, DLP12 prophage, UPF0098 family secreted protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/41);  total (43/41)

GTTTAAAGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAGGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGTTAT  >  NC_000913/571312‑571578                                                                                                                                     |                                                                                                                                       gTTTAAAGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTgag                                                                                                                                  >  1:1261624/1‑139 (MQ=255)     aaaGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGt                                                                                                                                     <  1:46590/132‑1 (MQ=255)     aaaGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGt                                                                                                                                     >  2:46590/1‑132 (MQ=255)       aGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATg                                                                                                                            <  1:312712/139‑1 (MQ=255)       aGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATg                                                                                                                            <  2:1261624/139‑1 (MQ=255)            gggCTCTATAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGGTTATGAGATAAAGTAAGGTGAGAGTATGCTGGc                                                                                                                       >  2:368698/1‑139 (MQ=255)            gggCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGc                                                                                                                       >  1:797398/1‑139 (MQ=255)            gggCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGc                                                                                                                       >  2:118298/1‑139 (MQ=255)             ggCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      <  2:797398/139‑1 (MQ=255)              gCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCaa                                                                                                                     <  1:118298/139‑1 (MQ=255)              gCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCaa                                                                                                                     <  1:992870/139‑1 (MQ=255)                    aaaaCTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTa                                                                                                               >  2:139352/1‑139 (MQ=255)                            aaaGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGCTAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAAc                                                                                                       >  2:294404/1‑139 (MQ=255)                            aaaGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      >  1:584007/1‑124 (MQ=255)                            aaaGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      <  2:584007/124‑1 (MQ=255)                                    cTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGAt                                                                                               <  2:231449/139‑1 (MQ=255)                                          aTTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      <  1:260665/110‑1 (MQ=255)                                          aTTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGa                                                                                                            <  2:1211591/120‑1 (MQ=255)                                          aTTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGa                                                                                                            >  1:1211591/1‑120 (MQ=255)                                          aTTCTAACTCCAGCGGAGCGTTAGGTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      >  2:260665/1‑110 (MQ=255)                                           ttCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCga                                                                                            <  1:909759/135‑1 (MQ=255)                                           ttCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCga                                                                                            >  2:909759/1‑135 (MQ=255)                                            tCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGaaa                                                                                       >  1:723578/1‑139 (MQ=255)                                                aCTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACg                                                                                   <  2:723578/139‑1 (MQ=255)                                                 cTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          >  1:99800/1‑115 (MQ=255)                                                 cTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          <  2:99800/115‑1 (MQ=255)                                                 cTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACgg                                                                                  <  1:368698/139‑1 (MQ=255)                                                  tCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGa                                                                                 <  2:501880/139‑1 (MQ=255)                                                    cAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGa                                                                                                            >  2:1030631/1‑110 (MQ=255)                                                    cAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGa                                                                                                            <  1:1030631/110‑1 (MQ=255)                                                     aGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGc                                                                              >  2:249933/1‑139 (MQ=255)                                                      gCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATg                                                                                                                            <  1:1183024/92‑1 (MQ=255)                                                      gCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATg                                                                                                                            >  2:1183024/1‑92 (MQ=255)                                                      gCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCaa                                                                                                                     <  1:435987/99‑1 (MQ=255)                                                      gCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCaa                                                                                                                     >  2:435987/1‑99 (MQ=255)                                                      gCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          >  2:843405/1‑110 (MQ=255)                                                      gCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          <  1:843405/110‑1 (MQ=255)                                                            cGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCaa                                                                                                                     <  2:792866/93‑1 (MQ=255)                                                            cGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCaa                                                                                                                     >  1:792866/1‑93 (MQ=255)                                                            cGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACgta                                                                                 >  1:1138087/1‑127 (MQ=255)                                                            cGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACgta                                                                                 <  2:1138087/129‑3 (MQ=255)                                                                    ggTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATgaga                                                               <  2:198306/139‑1 (MQ=255)                                                                      ttATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGAtt                                                             >  1:149793/1‑139 (MQ=255)                                                                        atatGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCa                                                           <  2:149793/139‑1 (MQ=255)                                                                                aaTGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTa                                                   >  1:519798/1‑139 (MQ=255)                                                                                aaTGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTa                                                   >  1:544733/1‑139 (MQ=255)                                                                                aaTGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTa                                                   >  1:806804/1‑139 (MQ=255)                                                                                       ataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCctct                                            >  1:1192408/1‑139 (MQ=255)                                                                                       ataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCctct                                            >  1:682604/1‑139 (MQ=255)                                                                                       ataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCctct                                            >  1:842999/1‑139 (MQ=255)                                                                                        taaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTgaga                                                                                                                                 <  1:1350669/53‑1 (MQ=255)                                                                                        taaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTgaga                                                                                                                                 >  2:1350669/1‑53 (MQ=255)                                                                                            aTCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATaaa                                                                         >  2:324690/1‑105 (MQ=255)                                                                                            aTCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATaaa                                                                         <  1:324690/105‑1 (MQ=255)                                                                                            aTCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGc                                       <  1:294404/139‑1 (MQ=255)                                                                                             tCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      <  2:268854/59‑1 (MQ=255)                                                                                             tCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      >  1:268854/1‑59 (MQ=255)                                                                                              cGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCa                                     >  2:214813/1‑139 (MQ=255)                                                                                                 aaCCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATaaa                                                                         >  1:548205/1‑100 (MQ=255)                                                                                                 aaCCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATaaa                                                                         <  2:548205/100‑1 (MQ=255)                                                                                                     gCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTtgt                              <  1:214813/139‑1 (MQ=255)                                                                                                           aTAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTa                        <  2:1192408/139‑1 (MQ=255)                                                                                                            tAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTAt                       <  2:682604/139‑1 (MQ=255)                                                                                                                  cAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCa                 <  2:519798/139‑1 (MQ=255)                                                                                                                   aGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATa                                                                                     >  1:11897/1‑70 (MQ=255)                                                                                                                   aGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATa                                                                                     <  2:11897/70‑1 (MQ=255)                                                                                                                    gTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCt                                  >  1:342537/1‑120 (MQ=255)                                                                                                                    gTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCt                                  <  2:342537/120‑1 (MQ=255)                                                                                                                     tttATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCgaag                                                                                          <  2:985877/63‑1 (MQ=255)                                                                                                                     tttATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCctct                                            <  2:496998/109‑1 (MQ=255)                                                                                                                     tttATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCctct                                            >  1:496998/1‑109 (MQ=255)                                                                                                                     tttATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAggg              >  1:491735/1‑139 (MQ=255)                                                                                                                     tttATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAggg              >  1:175824/1‑139 (MQ=255)                                                                                                                     tttATGAGATAAAGTAAGGTGAGAGTATGATGGCAAGAGGTAAGACTAACTTAAAGATCgaag                                                                                          >  1:985877/1‑63 (MQ=255)                                                                                                                          gagaTAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGGAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGgaaaga         >  1:359102/1‑139 (MQ=255)                                                                                                                          gagaTAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCAt                                                          >  2:581714/1‑90 (MQ=255)                                                                                                                          gagaTAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCAt                                                          <  1:581714/90‑1 (MQ=255)                                                                                                                             aTAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          >  2:306053/1‑39 (MQ=255)                                                                                                                             aTAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          <  1:306053/39‑1 (MQ=255)                                                                                                                              tAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTtgt                              >  1:1382198/1‑114 (MQ=255)                                                                                                                              tAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTtgt                              <  2:1382198/114‑1 (MQ=255)                                                                                                                              tAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGt     >  1:847156/1‑139 (MQ=255)                                                                                                                               aaaGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGtt    <  2:806804/139‑1 (MQ=255)                                                                                                                                 aGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGTTAt  <  1:1318299/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       GTTTAAAGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAGGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGTTAT  >  NC_000913/571312‑571578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑