Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	582,152	C→T	pseudogene (653/653 nt)	ybcY ←	pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	582,152	0	C	T	100.0%	259.9 / NA	80	pseudogene (653/653 nt)	ybcY	pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/39);  total (41/39)

CGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCT  >  NC_000913/582023‑582282                                                                                                                                  |                                                                                                                                   cGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTc                                                                                                                          <  1:489671/139‑1 (MQ=255)              aCCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                             <  1:1353329/139‑1 (MQ=255)                  tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                             <  1:280999/135‑1 (MQ=255)                  tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                             >  2:280999/1‑135 (MQ=255)                  tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                         >  2:1200514/1‑139 (MQ=255)                         aCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGaa                                                                                                                     >  2:153814/1‑120 (MQ=255)                         aCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGaa                                                                                                                     <  1:153814/120‑1 (MQ=255)                            tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                  <  2:1292153/136‑1 (MQ=255)                            tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                  >  1:1292153/1‑136 (MQ=255)                             cAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaaa                                                                                                              >  2:50615/1‑123 (MQ=255)                             cAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaaa                                                                                                              <  1:50615/123‑1 (MQ=255)                              aaCTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCg                                                                                                                  <  1:1059155/118‑1 (MQ=255)                              aaCTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCg                                                                                                                  >  2:1059155/1‑118 (MQ=255)                              aaCTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCt                                                                                             >  1:647234/1‑139 (MQ=255)                                   cACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAc                                                                                        >  2:296006/1‑139 (MQ=255)                                    aCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                       <  2:647234/139‑1 (MQ=255)                                    aCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                       <  1:560648/139‑1 (MQ=255)                                     ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                      <  2:97128/139‑1 (MQ=255)                                              gAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                             <  2:164335/139‑1 (MQ=255)                                                   gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                        >  2:329632/1‑139 (MQ=255)                                                   gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                        >  2:599114/1‑139 (MQ=255)                                                   gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTGTTAACATtc                                                                        >  2:189910/1‑139 (MQ=255)                                                    ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                       >  2:1243/1‑139 (MQ=255)                                                    ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                       >  2:370570/1‑139 (MQ=255)                                                      tACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCg                                                                     >  2:388925/1‑139 (MQ=255)                                                         gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                  <  2:91560/139‑1 (MQ=255)                                                             ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                              <  1:599114/139‑1 (MQ=255)                                                              cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                   >  2:748661/1‑101 (MQ=255)                                                              cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                   <  1:748661/101‑1 (MQ=255)                                                               tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                          >  2:440432/1‑93 (MQ=255)                                                               tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                          <  1:440432/93‑1 (MQ=255)                                                               tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                            <  2:256936/139‑1 (MQ=255)                                                                gTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                          <  1:275970/92‑1 (MQ=255)                                                                gTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                          >  2:275970/1‑92 (MQ=255)                                                                       aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                    >  1:431370/1‑139 (MQ=255)                                                                           aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACTGTACTACAg                                                                                                  >  1:666192/1‑89 (MQ=255)                                                                           aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                  <  2:666192/89‑1 (MQ=255)                                                                           aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACAt                                                                          >  1:459608/1‑113 (MQ=255)                                                                           aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACAt                                                                          <  2:459608/113‑1 (MQ=255)                                                                           aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgt                                                >  1:155852/1‑139 (MQ=255)                                                                               ttGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGaa                                                                                                                     >  1:698107/1‑66 (MQ=255)                                                                               ttGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGaa                                                                                                                     <  2:698107/66‑1 (MQ=255)                                                                                 gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgaga                                                        <  1:553871/125‑1 (MQ=255)                                                                                 gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgaga                                                        >  2:553871/1‑125 (MQ=255)                                                                                           cGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCgg                                <  1:1200514/139‑1 (MQ=255)                                                                                            gcagcaCGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGa                               <  1:296006/139‑1 (MQ=255)                                                                                             cagcaCGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                   >  1:951100/1‑70 (MQ=255)                                                                                             cagcaCGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                   <  2:951100/70‑1 (MQ=255)                                                                                             cagcaCGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                             <  2:987409/92‑1 (MQ=255)                                                                                             cagcaCGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                             >  1:987409/1‑92 (MQ=255)                                                                                             cagcaCGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                              >  2:679317/1‑139 (MQ=255)                                                                                                        aaGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTTAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGtt                   <  1:189910/139‑1 (MQ=255)                                                                                                        aaGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAg                                                                                                                    <  1:1096391/42‑1 (MQ=37)                                                                                                        aaGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAg                                                                                                                    >  2:1096391/1‑42 (MQ=37)                                                                                                         aGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGttt                  <  1:370570/139‑1 (MQ=255)                                                                                                              gAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                    <  1:883084/84‑1 (MQ=255)                                                                                                              gAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaaa             >  1:1176638/1‑139 (MQ=255)                                                                                                              gAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTAAAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                    >  2:883084/1‑84 (MQ=255)                                                                                                               aaCATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                        >  2:1297589/1‑79 (MQ=255)                                                                                                               aaCATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                        <  1:1297589/79‑1 (MQ=255)                                                                                                               aaCATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg            >  1:102579/1‑139 (MQ=255)                                                                                                               aaCATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg            >  2:371721/1‑139 (MQ=255)                                                                                                               aaCATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg            >  1:4569/1‑139 (MQ=255)                                                                                                                aCATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                             >  2:166081/1‑73 (MQ=255)                                                                                                                aCATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                             <  1:166081/73‑1 (MQ=255)                                                                                                                   tgctgcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                  <  1:1333428/49‑1 (MQ=38)                                                                                                                   tgctgcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                  >  2:1333428/1‑49 (MQ=38)                                                                                                                   tgctgcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                            >  2:627012/1‑87 (MQ=255)                                                                                                                   tgctgcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                            <  1:627012/87‑1 (MQ=255)                                                                                                                    gctgcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGcc       >  1:183974/1‑139 (MQ=255)                                                                                                                    gctgcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGcc       >  1:1236244/1‑139 (MQ=255)                                                                                                                     ctgcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                   >  1:288230/1‑94 (MQ=255)                                                                                                                     ctgcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                   <  2:288230/94‑1 (MQ=255)                                                                                                                       gcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt    <  2:4569/139‑1 (MQ=255)                                                                                                                       gcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt    <  2:431370/139‑1 (MQ=255)                                                                                                                       gcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt    <  2:155852/139‑1 (MQ=255)                                                                                                                        cGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACTGTACTTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCATCTTCGGAATCTTTTGTGTTTGAAATGATGCCTTTc   >  1:653922/1‑139 (MQ=255)                                                                                                                          ggttgATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCt  <  2:183974/135‑1 (MQ=255)                                                                                                                               aTGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt    >  2:1261461/1‑132 (MQ=255)                                                                                                                               aTGTTAGTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt    <  1:1261461/132‑1 (MQ=255)                                                                                                                                  |                                                                                                                                   CGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCT  >  NC_000913/582023‑582282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑