Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 801,638 A→G T294A (ACC→GCC)  ybhH → putative PrpF family isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913801,6380AG100.0% 292.3 / NA 81T294A (ACC→GCC) ybhHputative PrpF family isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/40);  total (41/40)

CCTAAACCTGTGCTTATTTCTCCAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGA  >  NC_000913/801503‑801770
                                                                                                                                       |                                                                                                                                    
ccTAAACCTGTGCTTATTTCTCCAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCg                                                                                                                                   >  2:438713/1‑139 (MQ=255)
    aaCCTGTGCTTATTTCTCCAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCAc                                                                                                                               >  1:1311823/1‑139 (MQ=255)
          tgCTTATTTCTCCAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACa                                                                                                                         >  2:149999/1‑139 (MQ=255)
          tgCTTATTTCTCCAGCGCAGAAAGGCGCGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACa                                                                                                                         >  1:222583/1‑139 (MQ=255)
              tATTTCTCCAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATc                                                                                                                     <  2:1050142/139‑1 (MQ=255)
                  tctcCAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTcc                                                                                                                 <  1:316613/139‑1 (MQ=255)
                   ctcCAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTccc                                                                                                                <  2:1311823/139‑1 (MQ=255)
                   ctcCAGCGCAGAAAGGCGCGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTccc                                                                                                                <  2:222583/139‑1 (MQ=255)
                     ccAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCtt                                                                                                              <  2:1373845/139‑1 (MQ=255)
                        gcgcAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTg                                                                                                           <  1:451058/139‑1 (MQ=255)
                         cgcAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGt                                                                                                          >  1:499537/1‑139 (MQ=255)
                         cgcAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGt                                                                                                          >  1:1425842/1‑139 (MQ=255)
                         cgcAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGATATTGCTATTTCCAGTAGTTGTGCATTCGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGt                                                                                                          >  1:862711/1‑139 (MQ=255)
                          gcAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTa                                                                                                         <  1:294005/139‑1 (MQ=255)
                             gAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGa                                                                                                      <  1:145158/139‑1 (MQ=255)
                                   cGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGc                                                                                                <  1:149999/139‑1 (MQ=255)
                                        cAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGcaatat                                                                                           <  1:1197308/139‑1 (MQ=255)
                                           ttAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTTGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGc                                                                                                >  1:357232/1‑131 (MQ=255)
                                           ttAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGc                                                                                                <  2:357232/131‑1 (MQ=255)
                                             aaTGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGcaatatcaata                                                                                      <  2:499537/139‑1 (MQ=255)
                                                gtgCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTg                                                                                   >  2:1078052/1‑139 (MQ=255)
                                                gtgCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTg                                                                                   <  2:275352/139‑1 (MQ=255)
                                                 tgCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGa                                                                                  >  1:1044164/1‑139 (MQ=255)
                                                  gCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGaa                                                                                 <  1:149622/139‑1 (MQ=255)
                                                   cGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAac                                                                                >  1:733690/1‑139 (MQ=255)
                                                    gTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGaa                                                                                 >  2:496506/1‑137 (MQ=255)
                                                    gTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGaa                                                                                 <  1:496506/137‑1 (MQ=255)
                                                     ttATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTg                                                                                                           <  1:221754/110‑1 (MQ=255)
                                                     ttATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTg                                                                                                           >  2:221754/1‑110 (MQ=255)
                                                       aTTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACAccc                                                                            <  1:187835/139‑1 (MQ=255)
                                                           tATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAgt                                                                        >  2:41434/1‑139 (MQ=255)
                                                             tGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCCTTGGAAGGCGCCGTCACCCGACAAATCGt                                                                                                                   <  2:694566/94‑1 (MQ=255)
                                                             tGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGt                                                                                                                   >  1:694566/1‑94 (MQ=255)
                                                                   aTTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCa                                                                          <  2:1010597/129‑1 (MQ=255)
                                                                   aTTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTCCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCa                                                                          >  1:1010597/1‑129 (MQ=255)
                                                                      cTTGCCATCGCGCGCTGGCGATAACCGGAGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACACCAGTGGTGCGCTCGa                                                             >  2:281823/1‑139 (MQ=255)
                                                                            aTCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATc                                                                                                                     <  1:1427595/77‑1 (MQ=255)
                                                                            aTCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATc                                                                                                                     >  2:1427595/1‑77 (MQ=255)
                                                                             tCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCAt                                                      <  2:1044164/139‑1 (MQ=255)
                                                                              cgcgcgCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGaa                                                                                 >  1:1045360/1‑111 (MQ=255)
                                                                              cgcgcgCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGaa                                                                                 <  2:1045360/111‑1 (MQ=255)
                                                                              cgcgcgCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCAtt                                                     <  1:438713/139‑1 (MQ=255)
                                                                               gcgcgcTGGCGATAACCGGTGCTATTGCTATTTCCAGTCGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTg                                                                                   >  2:770678/1‑108 (MQ=255)
                                                                               gcgcgcTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGc                                                                                                >  2:520970/1‑95 (MQ=255)
                                                                               gcgcgcTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGc                                                                                                <  1:520970/95‑1 (MQ=255)
                                                                               gcgcgcTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTg                                                                                   <  1:770678/108‑1 (MQ=255)
                                                                                       gCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATg                                            <  2:1425842/139‑1 (MQ=255)
                                                                                       gCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATg                                            <  1:41434/139‑1 (MQ=255)
                                                                                             aCCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGaa                                                                                 <  2:1282857/96‑1 (MQ=255)
                                                                                             aCCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGaa                                                                                 >  1:1282857/1‑96 (MQ=255)
                                                                                                 gTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCt                                                                <  2:364289/109‑1 (MQ=255)
                                                                                                 gTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCAt                                                      <  1:25208/119‑1 (MQ=255)
                                                                                                 gTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCAt                                                      >  2:25208/1‑119 (MQ=255)
                                                                                                 gTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCAAGTGGTGCGCt                                                                >  1:364289/1‑109 (MQ=255)
                                                                                                       ttgctattTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTg                                                                                                           <  1:484578/60‑1 (MQ=255)
                                                                                                       ttgctattTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTg                                                                                                           >  2:484578/1‑60 (MQ=255)
                                                                                                       ttgctattTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGcc                              >  1:2741/1‑137 (MQ=255)
                                                                                                       ttgctattTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGcc                              <  2:2741/137‑1 (MQ=255)
                                                                                                         gctattTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCacga                          >  2:1299928/1‑139 (MQ=255)
                                                                                                            attTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCg                                                                                                                            <  2:1202394/38‑1 (MQ=255)
                                                                                                            attTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCg                                                                                                                            >  1:1202394/1‑38 (MQ=255)
                                                                                                            attTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACg                                                           <  2:137352/103‑1 (MQ=255)
                                                                                                            attTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACg                                                           >  1:137352/1‑103 (MQ=255)
                                                                                                                  agtagtTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTAcgcg                 >  1:362592/1‑139 (MQ=255)
                                                                                                                   gtagtTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACgcgc                >  1:1099486/1‑139 (MQ=255)
                                                                                                                     agtTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTg                                                                                                           <  2:939700/46‑1 (MQ=255)
                                                                                                                     agtTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTg                                                                                                           >  1:939700/1‑46 (MQ=255)
                                                                                                                       ttGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGaa                                                                                 <  2:1051928/70‑1 (MQ=255)
                                                                                                                       ttGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGaa                                                                                 >  1:1051928/1‑70 (MQ=255)
                                                                                                                         gtgCATTGGAAGGCGCCGTCACCCGACAAATCGTccc                                                                                                                <  1:1291553/37‑1 (MQ=255)
                                                                                                                         gtgCATTGGAAGGCGCCGTCACCCGACAAATCGTccc                                                                                                                >  2:1291553/1‑37 (MQ=255)
                                                                                                                         gtgCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGa                                                                                                      <  2:1107315/47‑1 (MQ=255)
                                                                                                                         gtgCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGa                                                                                                      >  1:1107315/1‑47 (MQ=255)
                                                                                                                         gtgCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGt          >  1:291793/1‑139 (MQ=255)
                                                                                                                         gtgCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGt          >  1:544513/1‑139 (MQ=255)
                                                                                                                               tGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGc                                                                                                >  1:89532/1‑47 (MQ=255)
                                                                                                                               tGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGc                                                                                                <  2:89532/47‑1 (MQ=255)
                                                                                                                                ggAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCgg   >  2:959813/1‑139 (MQ=255)
                                                                                                                                 gAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAg                                         >  2:917673/1‑100 (MQ=255)
                                                                                                                                 gAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAg                                         <  1:917673/100‑1 (MQ=255)
                                                                                                                                 gAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGga  >  2:958108/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                    
CCTAAACCTGTGCTTATTTCTCCAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGA  >  NC_000913/801503‑801770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑