Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	829,972	C→T	M1M (GTG→ATG) † *224* (TAG→TAA)	ybhG ← ybiH ←	putative membrane fusion protein (MFP) component of efflux pump, membrane anchor DUF1956 domain‑containing tetR family putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	829,972	0	C	T	100.0%	163.8 / NA	52	M1M (GTG→ATG) † *224* (TAG→TAA)	ybhG ybiH	putative membrane fusion protein (MFP) component of efflux pump, membrane anchor DUF1956 domain‑containing tetR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/27);  total (25/27)

TACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTG  >  NC_000913/829838‑830090                                                                                                                                       |                                                                                                                       tACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACa                                                                                                                    <  1:1252644/139‑1 (MQ=255)  aCCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACaa                                                                                                                   >  1:164330/1‑139 (MQ=255)    cGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAAc                                                                                                                 >  1:851701/1‑139 (MQ=255)                   gTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATc                                                                                                  <  2:1225340/139‑1 (MQ=255)                     tGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCt                                                                                                             >  1:245158/1‑126 (MQ=255)                     tGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCt                                                                                                             <  2:245158/126‑1 (MQ=255)                     tGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCt                                                                                                >  2:246236/1‑139 (MQ=255)                      gCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCt                                                                                                             >  2:1259905/1‑125 (MQ=255)                      gCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCt                                                                                                             <  1:1259905/125‑1 (MQ=255)                            cAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCaaaa                                                                                         <  1:246236/139‑1 (MQ=255)                               cGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAATATCa                                                                                      >  1:942309/1‑139 (MQ=255)                               cGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                      >  2:188140/1‑139 (MQ=255)                               cGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                      >  2:578219/1‑139 (MQ=255)                               cGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                      >  1:50266/1‑139 (MQ=255)                               cGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                      >  2:1309673/1‑139 (MQ=255)                               cGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                      >  2:1367734/1‑139 (MQ=255)                               cGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                      >  2:153731/1‑139 (MQ=255)                                gTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                     <  2:939885/139‑1 (MQ=255)                                gTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                     <  1:16666/139‑1 (MQ=255)                                gTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                     <  2:831234/139‑1 (MQ=255)                                 tCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAgg                                                                                    >  1:942537/1‑139 (MQ=255)                                     gCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGa                                                                                <  2:942537/139‑1 (MQ=255)                                        gTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGatat                                                                             <  1:320107/139‑1 (MQ=255)                                         ttATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATg                                                                            >  1:693386/1‑139 (MQ=255)                                                    gcTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCg                                                                 <  2:1178553/139‑1 (MQ=255)                                                      tttGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGata                                                                              <  1:1186831/124‑1 (MQ=255)                                                      tttGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGata                                                                              >  2:1186831/1‑124 (MQ=255)                                                      tttGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTc                                                               <  2:50266/139‑1 (MQ=255)                                                                  aGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGatat                                                                             <  2:1249841/113‑1 (MQ=255)                                                                  aGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGatat                                                                             >  1:1249841/1‑113 (MQ=255)                                                                  aGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGt                                                   <  1:1367734/139‑1 (MQ=255)                                                                   gTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGtt                                                  <  1:188140/139‑1 (MQ=255)                                                                           cGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCttt                                          >  2:531694/1‑139 (MQ=255)                                                                                     ggcggcAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGa                                <  1:1309673/139‑1 (MQ=255)                                                                                         gcAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAAcgc                            <  1:1215658/139‑1 (MQ=255)                                                                                           aaGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCgg                          <  1:578219/139‑1 (MQ=255)                                                                                                   cTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTg                                                                                              <  1:1097727/63‑1 (MQ=255)                                                                                                   cTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTg                                                                                              >  2:1097727/1‑63 (MQ=255)                                                                                                   cTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCaaa                                                                                          <  2:1136158/67‑1 (MQ=255)                                                                                                   cTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCaaa                                                                                          >  1:1136158/1‑67 (MQ=255)                                                                                                      ccGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATc                                                                                       <  2:139077/67‑1 (MQ=255)                                                                                                      ccGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATc                                                                                       >  1:139077/1‑67 (MQ=255)                                                                                                        gCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATc                                                                                                  <  2:50892/54‑1 (MQ=255)                                                                                                        gCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATc                                                                                                  >  1:50892/1‑54 (MQ=255)                                                                                                        gCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACg             >  2:360748/1‑139 (MQ=255)                                                                                                         ccAATCCGATCACGATAGGTTTTTTCATCATTACAAACTCCtt                                                                                                            <  2:852233/43‑1 (MQ=255)                                                                                                         ccAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCtt                                                                                                            >  1:852233/1‑43 (MQ=255)                                                                                                         ccAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGatat                                                                             >  2:1056973/1‑74 (MQ=255)                                                                                                         ccAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGatat                                                                             <  1:1056973/74‑1 (MQ=255)                                                                                                             tCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAAt                                                                                        >  2:470940/1‑59 (MQ=255)                                                                                                             tCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAAt                                                                                        <  1:470940/59‑1 (MQ=255)                                                                                                                   cACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTg  <  2:164330/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                       TACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTG  >  NC_000913/829838‑830090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑