Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	967,118	A→G	Q166Q (CAA→CAG)	msbA →	lipid ABC transporter permease/ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	967,118	0	A	G	100.0%	191.8 / NA	54	Q166Q (CAA→CAG)	msbA	lipid ABC transporter permease/ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/25);  total (29/25)

GTACGCTGTTGTCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAAC  >  NC_000913/966985‑967245                                                                                                                                      |                                                                                                                                gTACGCTGTTGTCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTc                                                                                                                            >  1:201330/1‑139 (MQ=255)                 tATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATtgtg                                                                                                            <  2:201330/139‑1 (MQ=255)                     aCCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGAt                                                                                                                <  2:744799/131‑1 (MQ=255)                     aCCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATAGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGAt                                                                                                                >  1:744799/1‑131 (MQ=255)                          cGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCg                                                                                                   >  1:69200/1‑139 (MQ=255)                           gATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCt                                                                                                          >  1:113408/1‑131 (MQ=255)                           gATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCt                                                                                                          <  2:113408/131‑1 (MQ=255)                                       gTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTg                                                                                      >  1:781037/1‑139 (MQ=255)                                            ttcttcttcttcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGAtt                                                                                 >  1:296395/1‑139 (MQ=255)                                                     ttcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTa                                                                        >  1:501398/1‑139 (MQ=255)                                                     ttcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTa                                                                        >  1:539845/1‑139 (MQ=255)                                                          gcgcACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCt                                                                                                          >  2:756125/1‑100 (MQ=255)                                                          gcgcACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCt                                                                                                          <  1:756125/100‑1 (MQ=255)                                                              aCTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGt                                                               <  1:1229555/139‑1 (MQ=255)                                                               cTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGtt                                                              <  2:1000944/139‑1 (MQ=255)                                                                            tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAg                                                 >  1:981863/1‑139 (MQ=255)                                                                               gTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTaa                                              <  1:1252248/139‑1 (MQ=255)                                                                                   aGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcg                                                                               <  2:384909/102‑1 (MQ=255)                                                                                   aGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcg                                                                               >  1:384909/1‑102 (MQ=255)                                                                                                    gCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCgct                                                                                                                         >  1:948371/1‑41 (MQ=255)                                                                                                    gCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGAt                                                                                                                         <  2:627379/43‑1 (MQ=255)                                                                                                    gCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGAt                                                                                                                         >  1:627379/1‑43 (MQ=255)                                                                                                    gCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGAt                                                                                                                         <  2:948371/43‑1 (MQ=255)                                                                                                      cTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGc                       <  2:981863/139‑1 (MQ=255)                                                                                                      cTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATGTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGc                       <  2:781037/139‑1 (MQ=255)                                                                                                                tgatgTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGaccac             <  1:707859/139‑1 (MQ=255)                                                                                                                   tgTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACa                                                     >  1:523311/1‑96 (MQ=255)                                                                                                                   tgTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACa                                                     <  2:523311/96‑1 (MQ=255)                                                                                                                   tgTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAg          >  2:205823/1‑139 (MQ=255)                                                                                                                      tCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAAc                                          <  1:1192285/104‑1 (MQ=255)                                                                                                                      tCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAAc                                          >  2:1192285/1‑104 (MQ=255)                                                                                                                      tCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACAt                                        >  1:798741/1‑106 (MQ=255)                                                                                                                      tCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACAt                                        <  2:798741/106‑1 (MQ=255)                                                                                                                        tATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAg     >  2:547208/1‑139 (MQ=255)                                                                                                                          ttACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGaa   >  2:461731/1‑139 (MQ=255)                                                                                                                          ttACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGaa   >  2:338031/1‑139 (MQ=255)                                                                                                                             cAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCa                             <  1:1105435/110‑1 (MQ=255)                                                                                                                             cAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCa                             >  2:1105435/1‑110 (MQ=255)                                                                                                                               gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACTGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAAc                                                      >  2:1120360/1‑83 (MQ=255)                                                                                                                               gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                             <  1:1407797/60‑1 (MQ=255)                                                                                                                               gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                             >  2:1407797/1‑60 (MQ=255)                                                                                                                               gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGtt                                                                           >  2:371430/1‑62 (MQ=255)                                                                                                                               gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGtt                                                                           <  1:371430/62‑1 (MQ=255)                                                                                                                               gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAAc                                                      >  1:905193/1‑83 (MQ=255)                                                                                                                               gTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAAc                                                      <  2:905193/83‑1 (MQ=255)                                                                                                                               gTTGGCAGCTGTCGATCATTTTGATTGTGCCGGCACTGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAAc                                                      <  1:1120360/83‑1 (MQ=255)                                                                                                                                ttGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                             >  2:654179/1‑59 (MQ=255)                                                                                                                                ttGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                             <  1:654179/59‑1 (MQ=255)                                                                                                                                ttGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAAc  <  1:174653/134‑1 (MQ=255)                                                                                                                                ttGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAAc  >  2:174653/1‑134 (MQ=255)                                                                                                                                  ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGAtt                                                                                 >  2:1112974/1‑53 (MQ=255)                                                                                                                                  ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGAtt                                                                                 <  1:1112974/53‑1 (MQ=255)                                                                                                                                  ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                             <  2:193241/57‑1 (MQ=255)                                                                                                                                  ggCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                             >  1:193241/1‑57 (MQ=255)                                                                                                                                      |                                                                                                                                GTACGCTGTTGTCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAAC  >  NC_000913/966985‑967245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑