Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 969,352 G→A *329* (TAG→TAA lpxK → lipid A 4'kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913969,3520GA100.0% 87.6 / NA 29*329* (TAG→TAAlpxKlipid A 4'kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/12);  total (17/12)

CGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGA  >  NC_000913/969231‑969472
                                                                                                                         |                                                                                                                        
cGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGt                                                                                                         <  2:950658/139‑1 (MQ=255)
 ggTGAAATGCCGGGCCTTTGCAGGAGAAAATTGGTGCTATTTACTTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGGCGGCCTCTGGCAGCGAATTACGCCGCGGCAGCGtt                                                                                                        >  2:1366255/1‑139 (MQ=255)
             ggCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAg                                                                                            >  2:98809/1‑139 (MQ=255)
               ccTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATg                                                                                               >  1:282104/1‑134 (MQ=255)
               ccTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATg                                                                                               <  2:282104/134‑1 (MQ=255)
                                     gtATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGt                                                                                                         <  2:914102/102‑1 (MQ=255)
                                     gtATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGt                                                                                                         >  1:914102/1‑102 (MQ=255)
                                     gtATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACctc                                                                    >  1:1052211/1‑139 (MQ=255)
                                                tAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATgg                                                                                              >  1:1167844/1‑102 (MQ=255)
                                                tAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATgg                                                                                              <  2:1167844/102‑1 (MQ=255)
                                                tAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGa                                                         >  2:796842/1‑139 (MQ=255)
                                                     gCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGAt                                                                                                    >  1:896540/1‑91 (MQ=255)
                                                     gCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGAt                                                                                                    <  2:896540/91‑1 (MQ=255)
                                                                     atgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCGACTAATTACGCCGCGGCAGCGt                                                                                                         <  2:679017/70‑1 (MQ=255)
                                                                     atgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCGACTAATTACGCCGCGGCAGCGt                                                                                                         >  1:679017/1‑70 (MQ=255)
                                                                     atgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGc                                    <  1:98809/139‑1 (MQ=255)
                                                                     atgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGc                                    >  1:1356886/1‑139 (MQ=255)
                                                                     atgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGc                                    >  2:1053353/1‑139 (MQ=255)
                                                                             gCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGcc                                                                           <  1:856915/92‑1 (MQ=255)
                                                                             gCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGcc                                                                           >  2:856915/1‑92 (MQ=255)
                                                                                  aCTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTg                       <  2:1052211/139‑1 (MQ=255)
                                                                                              aCTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAAccc           >  2:809065/1‑139 (MQ=255)
                                                                                               cTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCg                                                                          >  1:747665/1‑75 (MQ=255)
                                                                                               cTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCg                                                                          <  2:747665/75‑1 (MQ=255)
                                                                                                       gCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGa                                                         >  2:258602/1‑84 (MQ=255)
                                                                                                       gCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGa                                                         <  1:258602/84‑1 (MQ=255)
                                                                                                       gCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGa  >  2:1094839/1‑139 (MQ=255)
                                                                                                             ttCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGa                                                                                             >  2:488450/1‑42 (MQ=255)
                                                                                                             ttCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGa                                                                                             <  1:488450/42‑1 (MQ=255)
                                                                                                                         |                                                                                                                        
CGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGA  >  NC_000913/969231‑969472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: