| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,125,011 | A→G | C106R (TGC→CGC) | mdtH ← | multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,125,011 | 0 | A | G | 100.0% | 169.0 / NA | 47 | C106R (TGC→CGC) | mdtH | multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/23); total (24/23) | |||||||||||
TCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAA > NC_000913/1124896‑1125135 | tCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAATAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGtt > 1:945516/1‑139 (MQ=255)tCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGtt > 1:86701/1‑139 (MQ=255) catcatCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTgg < 1:1296807/139‑1 (MQ=255) catcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCg < 1:252132/139‑1 (MQ=255) tcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACACCGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGc > 2:462383/1‑136 (MQ=255) tcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGc < 1:462383/136‑1 (MQ=255) tcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGAt > 2:1358570/1‑139 (MQ=255) gagaAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACcaca > 1:1231300/1‑109 (MQ=255) gagaAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACcaca < 2:1231300/109‑1 (MQ=255) gagaAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCg < 1:1221710/123‑1 (MQ=255) gagaAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCg > 2:1221710/1‑123 (MQ=255) gaAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATtgtg < 1:1076339/139‑1 (MQ=255) gctgTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGgcgcg < 2:1306497/139‑1 (MQ=255) aTTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGaa < 1:147485/72‑1 (MQ=255) aTTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGaa > 2:147485/1‑72 (MQ=255) aaTTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCgg < 2:86701/139‑1 (MQ=255) caccacCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAAc < 1:882283/67‑1 (MQ=255) caccacCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAAc > 2:882283/1‑67 (MQ=255) ccaccaGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAat > 1:748592/1‑139 (MQ=255) ccaccaGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAat > 1:1164593/1‑139 (MQ=255) gcCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGtt < 2:1164593/139‑1 (MQ=255) ccGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGCAACAATCATCGGttt > 1:896158/1‑139 (MQ=255) gAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCAt > 1:806822/1‑110 (MQ=255) gAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCAt < 2:806822/110‑1 (MQ=255) aaCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCg < 1:1131738/77‑1 (MQ=255) aaCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCg > 2:1131738/1‑77 (MQ=255) aaCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCAt < 1:1037654/109‑1 (MQ=255) aaCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCAt > 2:1037654/1‑109 (MQ=255) ggcggATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCCGcccac < 2:1356912/112‑5 (MQ=255) ggcggATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCCGcccac > 1:1356912/1‑108 (MQ=255) ggcggATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCaa > 2:1383023/1‑139 (MQ=255) gTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCa > 1:863976/1‑94 (MQ=255) gTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCa < 2:863976/94‑1 (MQ=255) tGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCAt > 2:1239535/1‑94 (MQ=255) tGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCAt < 1:1239535/94‑1 (MQ=255) gCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGgcggcg < 1:169504/82‑1 (MQ=255) gCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGgcggcg > 2:169504/1‑82 (MQ=255) ccACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCa < 1:1080509/41‑1 (MQ=255) ccACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCa > 2:1080509/1‑41 (MQ=255) cGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCAccgcc > 1:236797/1‑139 (MQ=255) aGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATa < 2:45302/87‑1 (MQ=255) aGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATa > 1:45302/1‑87 (MQ=255) aGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGa > 2:722740/1‑139 (MQ=255) tCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGgcggcg > 1:426853/1‑73 (MQ=255) tCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGgcggcg < 2:426853/73‑1 (MQ=255) tCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGaaa < 1:1383023/139‑1 (MQ=255) tCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGaaa < 1:722740/139‑1 (MQ=255) | TCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAA > NC_000913/1124896‑1125135 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |