Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	117,798	A→G	L283P (CTA→CCA)	nadC ←	quinolinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	117,798	0	A	G	100.0%	126.9 / NA	36	L283P (CTA→CCA)	nadC	quinolinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/17);  total (19/17)

CAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTAGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCATCTGTTCTGTTTC  >  NC_000913/117669‑117931                                                                                                                                  |                                                                                                                                      cAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGCCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGa                                                                                                                              >  2:703195/1‑139 (MQ=255)  aaGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGAc                                                                                                                             <  1:157530/139‑1 (MQ=255)                            aaGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCgg                                                                                                   >  2:418238/1‑139 (MQ=255)                                 ggggAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAAt                                                                                              >  1:924981/1‑139 (MQ=255)                                     aaagggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCAc                                                                                          >  1:260427/4‑139 (MQ=255)                                      aGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACg                                                                                         <  1:418238/139‑1 (MQ=255)                                         ggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTTACGCAg                                                                                      >  2:1381874/1‑139 (MQ=255)                                         ggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAg                                                                                      >  1:689151/1‑139 (MQ=255)                                             aTTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGCCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGtt                                                                                  <  1:703195/139‑1 (MQ=255)                                                     cccgcGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATa                                                                                                                      <  2:755014/95‑1 (MQ=255)                                                     cccgcGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATa                                                                                                                      >  1:755014/1‑95 (MQ=255)                                                         cgATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCa                                                                                       <  2:1108758/122‑1 (MQ=255)                                                         cgATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCa                                                                                       >  1:1108758/1‑122 (MQ=255)                                                                                   aTTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAgtg                                                                                    >  1:300590/1‑99 (MQ=255)                                                                                   aTTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAgtg                                                                                    <  2:300590/99‑1 (MQ=255)                                                                                    ttAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACg                                                                                                                            <  1:796791/58‑1 (MQ=255)                                                                                    ttAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACg                                                                                                                            >  2:796791/1‑58 (MQ=255)                                                                                       gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACg                                                                                                                            <  2:1348667/55‑1 (MQ=255)                                                                                       gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACg                                                                                                                            >  1:1348667/1‑55 (MQ=255)                                                                                           aaaCGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGa                                                                        >  1:1123061/1‑103 (MQ=255)                                                                                           aaaCGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGa                                                                        <  2:1123061/103‑1 (MQ=255)                                                                                            aaCGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGTCGGAGATAAAGTCCACGCCCGTTTCgg                                                                                                   <  1:1388989/75‑1 (MQ=255)                                                                                            aaCGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGTCGGAGATAAAGTCCACGCCCGTTTCgg                                                                                                   >  2:1388989/1‑75 (MQ=255)                                                                                            aaCGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTg                                                                               <  2:1365308/95‑1 (MQ=255)                                                                                            aaCGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTg                                                                               >  1:1365308/1‑95 (MQ=255)                                                                                                  ttGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                        <  1:526041/80‑1 (MQ=255)                                                                                                  ttGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                        >  2:526041/1‑80 (MQ=255)                                                                                                         gTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTTACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCt                      <  1:1381874/139‑1 (MQ=255)                                                                                                            gAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTcg                   <  2:260427/139‑1 (MQ=255)                                                                                                            gAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTcg                   <  2:689151/139‑1 (MQ=255)                                                                                                             aGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCt                                             >  2:1007880/1‑112 (MQ=255)                                                                                                             aGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCt                                             <  1:1007880/112‑1 (MQ=255)                                                                                                               tGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCg                                                                                                    <  1:548220/55‑1 (MQ=255)                                                                                                               tGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCg                                                                                                    >  2:548220/1‑55 (MQ=255)                                                                                                                 cTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCACCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCAt              >  1:499213/1‑139 (MQ=255)                                                                                                                    gTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCAtctg           >  1:1207572/1‑139 (MQ=255)                                                                                                                             tagttggCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCATCTGTTCTGTTTc  >  2:294297/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                      CAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTAGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCATCTGTTCTGTTTC  >  NC_000913/117669‑117931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑