Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,275,815	T→C	E5E (GAA→GAG)	narL ←	response regulator in two‑component regulatory system with NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,275,815	0	T	C	100.0%	247.5 / NA	68	E5E (GAA→GAG)	narL	response regulator in two‑component regulatory system with NarX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/34);  total (34/34)

TCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCG  >  NC_000913/1275684‑1275950                                                                                                                                    |                                                                                                                                        tCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGTATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGAt                                                                                                                                  >  2:929072/1‑139 (MQ=255)   cGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTa                                                                                                                                >  1:11505/1‑139 (MQ=255)   cGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTa                                                                                                                                <  2:11505/139‑1 (MQ=255)      cAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTc                                                                                                                             <  2:477693/139‑1 (MQ=255)       aGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCa                                                                                                                            <  2:1076515/139‑1 (MQ=255)          tCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATgg                                                                                                                         <  2:1177020/139‑1 (MQ=255)              tACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                     <  1:26250/139‑1 (MQ=255)                   tGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGt                                                                                                                       >  2:1266733/1‑132 (MQ=255)                   tGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGt                                                                                                                       <  1:1266733/132‑1 (MQ=255)                   tGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCt                                                                                                                >  2:948418/1‑139 (MQ=255)                   tGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCt                                                                                                                >  2:345850/1‑139 (MQ=255)                     ttCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGt                                                                                                                       <  1:349205/130‑1 (MQ=255)                     ttCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGt                                                                                                                       >  2:349205/1‑130 (MQ=255)                     ttCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTg                                                                                                              >  1:1035332/1‑139 (MQ=255)                     ttCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCCCCTGATTACTCATGGGTATCTCCTTg                                                                                                              >  2:772563/1‑139 (MQ=255)                     ttCGCCAATACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTg                                                                                                              >  1:1064064/1‑139 (MQ=255)                      tCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTAc                                                                                                                               >  1:1264423/1‑121 (MQ=255)                      tCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTAc                                                                                                                               <  2:1264423/121‑1 (MQ=255)                          cATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGt                                                                                                         <  2:53238/139‑1 (MQ=255)                             tACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTTCTGATTACTCATGGGTATCTCCTTgg                                                                                                             <  2:494267/132‑1 (MQ=255)                             tACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTgg                                                                                                             >  1:494267/1‑132 (MQ=255)                               cTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCtgtg                                                                                                    >  2:1214287/1‑139 (MQ=255)                                                  tGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATaa                                                                                 <  2:1035332/139‑1 (MQ=255)                                                          ggTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCt                                                                                                                                     <  1:280166/79‑1 (MQ=255)                                                          ggTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCt                                                                                                                                     >  2:280166/1‑79 (MQ=255)                                                            tGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGt                                                                             <  1:774845/133‑1 (MQ=255)                                                            tGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGt                                                                             >  2:774845/1‑133 (MQ=255)                                                                 tACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCCCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACtt                                                                  <  1:772563/139‑1 (MQ=255)                                                                     gATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGt                                                              <  2:200227/139‑1 (MQ=255)                                                                      aTAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTg                                                             <  1:1214287/139‑1 (MQ=255)                                                                              ttttACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTa                                                                                                                                >  2:1089534/1‑64 (MQ=255)                                                                              ttttACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTa                                                                                                                                <  1:1089534/64‑1 (MQ=255)                                                                              ttttACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTc                                                                                                                             <  1:255385/67‑1 (MQ=255)                                                                              ttttACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTc                                                                                                                             >  2:255385/1‑67 (MQ=255)                                                                              ttttACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTa                                                                                                                      <  1:1169591/74‑1 (MQ=255)                                                                              ttttACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTa                                                                                                                      >  2:1169591/1‑74 (MQ=255)                                                                              ttttACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGa                                                     >  2:700019/1‑139 (MQ=255)                                                                                        gTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGt                                                                                                                       <  2:597842/63‑1 (MQ=255)                                                                                        gTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGt                                                                                                                       >  1:597842/1‑63 (MQ=255)                                                                                        gTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGAcgg                                           >  2:1235253/1‑139 (MQ=255)                                                                                                caTCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCAGAcgcg                                   >  2:357486/1‑139 (MQ=255)                                                                                                  tCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCa                                                                                                                            >  1:1296244/1‑48 (MQ=255)                                                                                                  tCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCa                                                                                                                            <  2:1296244/48‑1 (MQ=255)                                                                                                  tCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTc                                                                                         >  1:1108392/1‑83 (MQ=255)                                                                                                  tCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTc                                                                                         <  2:1108392/83‑1 (MQ=255)                                                                                                  tCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGc                                 >  2:379452/1‑139 (MQ=255)                                                                                                   cGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCa                                >  2:1161996/1‑139 (MQ=255)                                                                                                     ggTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAAt                              >  1:94388/1‑139 (MQ=255)                                                                                                     ggTGATCGTCAATCAGCAGGATAGTAGCCGACTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAAt                              >  1:391597/1‑139 (MQ=255)                                                                                                        gATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGc                           >  1:1193820/1‑139 (MQ=255)                                                                                                              cAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTa                     <  1:357486/139‑1 (MQ=255)                                                                                                                  cagcagGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACt                 <  1:1331206/139‑1 (MQ=255)                                                                                                                        gATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTgcgc           >  2:442841/1‑139 (MQ=255)                                                                                                                        gATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTgcgc           >  2:562745/1‑139 (MQ=255)                                                                                                                         aTAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCa          <  1:379452/139‑1 (MQ=255)                                                                                                                         aTAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCa          <  2:94388/139‑1 (MQ=255)                                                                                                                          tagtagCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCAc         <  1:345850/139‑1 (MQ=255)                                                                                                                           agtagCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACg        >  2:987251/1‑139 (MQ=255)                                                                                                                            gtagCCGGCTCATGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGa       <  1:929072/139‑1 (MQ=255)                                                                                                                             tagCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGa       <  2:396209/138‑1 (MQ=255)                                                                                                                             tagCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGa       >  1:396209/1‑138 (MQ=255)                                                                                                                               gCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACt                                                                   <  2:1143982/76‑1 (MQ=255)                                                                                                                               gCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACt                                                                   >  1:1143982/1‑76 (MQ=255)                                                                                                                               gCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATcg    >  2:339036/1‑139 (MQ=255)                                                                                                                               gCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATcg    <  1:987251/139‑1 (MQ=255)                                                                                                                               gCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATcg    <  1:948418/139‑1 (MQ=255)                                                                                                                                 cGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATcgcg  <  1:1136882/139‑1 (MQ=255)                                                                                                                                 cGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATcgcg  <  1:339036/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        TCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCG  >  NC_000913/1275684‑1275950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑