Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,351,197	C→T	intergenic (‑158/+210)	fabI ← / ← ycjD	enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,351,197	0	C	T	100.0%	195.7 / NA	63	intergenic (‑158/+210)	fabI/ycjD	enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/33);  total (30/33)

CCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTT  >  NC_000913/1351072‑1351324                                                                                                                              |                                                                                                                                ccTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCa                                                                                                                    >  2:1198437/1‑139 (MQ=255)     aaaaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCt                                                                                                                <  1:596195/139‑1 (MQ=255)         tCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAAcc                                                                                                            <  2:519366/139‑1 (MQ=255)         tCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAAcc                                                                                                            <  1:51351/139‑1 (MQ=255)           aGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCct                                                                                                          <  2:487295/139‑1 (MQ=255)                             gTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                        <  1:249701/139‑1 (MQ=255)                                aaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa                                                                                     <  1:1198437/139‑1 (MQ=255)                                     tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                >  2:1250362/1‑139 (MQ=255)                                          aCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAAcc                                                                                                            <  2:52875/106‑1 (MQ=255)                                          aCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAAcc                                                                                                            >  1:52875/1‑106 (MQ=255)                                              aTAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaaa                                                                       >  2:781062/1‑139 (MQ=255)                                               tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                >  1:986289/1‑129 (MQ=255)                                               tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                <  2:986289/129‑1 (MQ=255)                                                        attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCttt                                                                                                                            >  2:1104704/1‑76 (MQ=255)                                                        attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCttt                                                                                                                            <  1:1104704/76‑1 (MQ=255)                                                        attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCt                                                                                                                      <  1:216529/82‑1 (MQ=255)                                                        attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCt                                                                                                                      >  2:216529/1‑82 (MQ=255)                                                        attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGc                                                                                            >  2:721519/1‑108 (MQ=255)                                                        attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGc                                                                                            <  1:721519/108‑1 (MQ=255)                                                        attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                <  2:1146183/120‑1 (MQ=255)                                                        attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCATCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                >  1:1146183/1‑120 (MQ=255)                                                                 aaCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGTGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                          <  1:239683/101‑1 (MQ=255)                                                                 aaCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                          >  2:239683/1‑101 (MQ=255)                                                                   ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCct                                                                                                          >  2:830594/1‑83 (MQ=255)                                                                   ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCct                                                                                                          <  1:830594/83‑1 (MQ=255)                                                                   ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                        >  1:1381207/1‑101 (MQ=255)                                                                   ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                        <  2:1381207/101‑1 (MQ=255)                                                                   ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                  >  1:431582/1‑139 (MQ=255)                                                                   ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                  <  2:431582/139‑1 (MQ=255)                                                                   ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                  <  1:781062/139‑1 (MQ=255)                                                                      ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa                                                                                     >  1:677500/1‑101 (MQ=255)                                                                      ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa                                                                                     <  2:677500/101‑1 (MQ=255)                                                                           tgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                <  2:901724/101‑1 (MQ=255)                                                                           tgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                >  1:901724/1‑101 (MQ=255)                                                                             aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATa                                        >  2:352589/1‑139 (MQ=255)                                                                             aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGACTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                >  1:1108157/1‑99 (MQ=255)                                                                             aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGACTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                <  2:1108157/99‑1 (MQ=255)                                                                              gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGTGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                >  1:485722/1‑98 (MQ=255)                                                                              gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGTGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                <  2:485722/98‑1 (MQ=255)                                                                                 gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAg                                                         <  2:434833/118‑1 (MQ=255)                                                                                 gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAg                                                         >  1:434833/1‑118 (MQ=255)                                                                                  cTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                          >  2:586652/1‑84 (MQ=255)                                                                                  cTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                          <  1:586652/84‑1 (MQ=255)                                                                                       gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                          <  2:23513/79‑1 (MQ=255)                                                                                       gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                          >  1:23513/1‑79 (MQ=255)                                                                                       gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa                                                                                     <  1:576414/84‑1 (MQ=255)                                                                                       gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa                                                                                     >  2:576414/1‑84 (MQ=255)                                                                                         tGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCATATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAcc                            >  2:64274/1‑139 (MQ=255)                                                                                             aGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGGTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa                                                                                     <  1:934069/78‑1 (MQ=255)                                                                                             aGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa                                                                                     >  2:934069/1‑78 (MQ=255)                                                                                               gTCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCa                      >  2:1122474/1‑139 (MQ=255)                                                                                                tCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                  >  1:721693/1‑110 (MQ=255)                                                                                                tCCGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                  <  2:721693/110‑1 (MQ=255)                                                                                                  cGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCt                                                                                                                      >  2:1423475/1‑40 (MQ=255)                                                                                                  cGACCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCt                                                                                                                      <  1:1423475/40‑1 (MQ=255)                                                                                                    aCCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa                                                                         <  2:1391668/83‑1 (MQ=255)                                                                                                    aCCGGAGCAGGCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa                                                                         >  1:1391668/1‑83 (MQ=255)                                                                                                              gCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa       <  1:352589/139‑1 (MQ=255)                                                                                                              gCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa       <  1:1250362/139‑1 (MQ=255)                                                                                                              gCTGCGGCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCATATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa       <  1:64274/139‑1 (MQ=255)                                                                                                                   ggCAGGGGGGGTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGtt  >  1:1171719/1‑139 (MQ=255)                                                                                                                        ggggggTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGtt                                                                <  2:661866/72‑1 (MQ=255)                                                                                                                        ggggggTGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGtt                                                                >  1:661866/1‑72 (MQ=255)                                                                                                                              |                                                                                                                                CCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTT  >  NC_000913/1351072‑1351324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑