Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,389,895 G→A *322* (TAG→TAA
P235L (CCT→CTT) 
ycjG →
mpaA ←
L‑Ala‑D/L‑Glu epimerase
murein peptide amidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,389,8950GA100.0% 113.5 / NA 36*322* (TAG→TAA
P235L (CCT→CTT) 
ycjG
mpaA
L‑Ala‑D/L‑Glu epimerase
murein peptide amidase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/17);  total (19/17)

GTGCCATTAGCGCCGCTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAGGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGG  >  NC_000913/1389771‑1390025
                                                                                                                            |                                                                                                                                  
gTGCCATTAGCGCCGCTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCgcagc                                                                                                                      >  2:421457/1‑139 (MQ=255)
               cTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCa                                                                                                       <  1:913005/139‑1 (MQ=255)
                       cTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGata                                                                                               >  2:973599/1‑139 (MQ=255)
                              cGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCg                                                                                        <  1:421457/139‑1 (MQ=255)
                                  ggTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGTCAGCGCAGCAAGTTTGCCATCGCa                                                                                                       >  2:839855/1‑120 (MQ=255)
                                  ggTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGTCAGCGCAGCAAGTTTGCCATCGCa                                                                                                       <  1:839855/120‑1 (MQ=255)
                                  ggTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTgg                                                                                    >  2:356348/1‑139 (MQ=255)
                                      aGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCTCTGGCTtc                                                                                >  1:5559/1‑139 (MQ=255)
                                                 tCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGgaga                                                                     <  2:113415/139‑1 (MQ=255)
                                                             gaccTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAAct                                                         >  1:1113139/1‑139 (MQ=255)
                                                             gaccTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAAct                                                         >  2:958694/1‑139 (MQ=255)
                                                                  ggctggcGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCg                                                    <  1:356348/139‑1 (MQ=255)
                                                                   gctggcGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGACACTCTGCgg                                                   <  1:958694/139‑1 (MQ=255)
                                                                   gctggcGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCgg                                                   <  2:1060703/139‑1 (MQ=255)
                                                                        cGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCa                                                                                                       >  1:836925/1‑82 (MQ=255)
                                                                        cGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCa                                                                                                       <  2:836925/82‑1 (MQ=255)
                                                                         ggTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGatgc                                             >  2:547814/1‑139 (MQ=255)
                                                                          gTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAagag                                                                                                  <  2:990257/85‑1 (MQ=255)
                                                                          gTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAagag                                                                                                  >  1:990257/1‑85 (MQ=255)
                                                                                 tgGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGgaga                                                                     <  2:12001/107‑1 (MQ=255)
                                                                                 tgGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGgaga                                                                     >  1:12001/1‑107 (MQ=255)
                                                                                         gcgcTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCAt                                                                                                           <  1:629159/61‑1 (MQ=255)
                                                                                         gcgcTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCAt                                                                                                           >  2:629159/1‑61 (MQ=255)
                                                                                         gcgcTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATAtttt                                                                                           >  1:633819/1‑77 (MQ=255)
                                                                                         gcgcTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATAtttt                                                                                           <  2:633819/77‑1 (MQ=255)
                                                                                              tCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTg                                                      <  2:208556/109‑1 (MQ=255)
                                                                                              tCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTg                                                      >  1:208556/1‑109 (MQ=255)
                                                                                                         cgGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGgaga                                                                     >  1:600383/1‑83 (MQ=255)
                                                                                                         cgGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGgaga                                                                     <  2:600383/83‑1 (MQ=255)
                                                                                                           ggCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGaa           >  2:561670/1‑139 (MQ=255)
                                                                                                            gCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGatgcaatgc                                        <  1:1321995/109‑1 (MQ=255)
                                                                                                            gCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGatgcaatgc                                        >  2:1321995/1‑109 (MQ=255)
                                                                                                               aaTTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAagag       >  1:1221309/1‑139 (MQ=255)
                                                                                                                    cATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCTCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAgg  <  2:5559/139‑1 (MQ=255)
                                                                                                                       cTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTg                >  2:1417487/1‑122 (MQ=255)
                                                                                                                       cTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTg                <  1:1417487/122‑1 (MQ=255)
                                                                                                                            |                                                                                                                                  
GTGCCATTAGCGCCGCTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAGGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGG  >  NC_000913/1389771‑1390025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: