Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,389,895 | G→A | *322* (TAG→TAA) P235L (CCT→CTT) |
ycjG → mpaA ← |
L‑Ala‑D/L‑Glu epimerase murein peptide amidase A |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,389,895 | 0 | G | A | 100.0% | 113.5 / NA | 36 | *322* (TAG→TAA) P235L (CCT→CTT) | ycjG mpaA | L‑Ala‑D/L‑Glu epimerase murein peptide amidase A |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/17); total (19/17) |
GTGCCATTAGCGCCGCTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAGGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGG > NC_000913/1389771‑1390025 | gTGCCATTAGCGCCGCTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCgcagc > 2:421457/1‑139 (MQ=255) cTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCa < 1:913005/139‑1 (MQ=255) cTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGata > 2:973599/1‑139 (MQ=255) cGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCg < 1:421457/139‑1 (MQ=255) ggTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGTCAGCGCAGCAAGTTTGCCATCGCa > 2:839855/1‑120 (MQ=255) ggTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGTCAGCGCAGCAAGTTTGCCATCGCa < 1:839855/120‑1 (MQ=255) ggTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTgg > 2:356348/1‑139 (MQ=255) aGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCTCTGGCTtc > 1:5559/1‑139 (MQ=255) tCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGgaga < 2:113415/139‑1 (MQ=255) gaccTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAAct > 1:1113139/1‑139 (MQ=255) gaccTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAAct > 2:958694/1‑139 (MQ=255) ggctggcGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCg < 1:356348/139‑1 (MQ=255) gctggcGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGACACTCTGCgg < 1:958694/139‑1 (MQ=255) gctggcGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCgg < 2:1060703/139‑1 (MQ=255) cGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCa > 1:836925/1‑82 (MQ=255) cGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCa < 2:836925/82‑1 (MQ=255) ggTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGatgc > 2:547814/1‑139 (MQ=255) gTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAagag < 2:990257/85‑1 (MQ=255) gTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAagag > 1:990257/1‑85 (MQ=255) tgGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGgaga < 2:12001/107‑1 (MQ=255) tgGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGgaga > 1:12001/1‑107 (MQ=255) gcgcTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCAt < 1:629159/61‑1 (MQ=255) gcgcTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCAt > 2:629159/1‑61 (MQ=255) gcgcTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATAtttt > 1:633819/1‑77 (MQ=255) gcgcTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATAtttt < 2:633819/77‑1 (MQ=255) tCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTg < 2:208556/109‑1 (MQ=255) tCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTg > 1:208556/1‑109 (MQ=255) cgGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGgaga > 1:600383/1‑83 (MQ=255) cgGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGgaga < 2:600383/83‑1 (MQ=255) ggCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGaa > 2:561670/1‑139 (MQ=255) gCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGatgcaatgc < 1:1321995/109‑1 (MQ=255) gCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGatgcaatgc > 2:1321995/1‑109 (MQ=255) aaTTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAagag > 1:1221309/1‑139 (MQ=255) cATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCTCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAgg < 2:5559/139‑1 (MQ=255) cTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTg > 2:1417487/1‑122 (MQ=255) cTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTg < 1:1417487/122‑1 (MQ=255) | GTGCCATTAGCGCCGCTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAGGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGG > NC_000913/1389771‑1390025 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |