Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,495,688	A→G	E134G (GAG→GGG)	ydcJ →	putative metalloenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,495,688	0	A	G	100.0%	154.6 / NA	43	E134G (GAG→GGG)	ydcJ	putative metalloenzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/19);  total (24/19)

TACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGAGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACAACTGTTAGAGGAATATGAGCAGCAGGGCGGTTTTAACGAAACACAGGCACAGG  >  NC_000913/1495561‑1495822                                                                                                                                |                                                                                                                                       tACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCgaga                                                                                                                             >  2:278147/1‑139 (MQ=255)  aCCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGaa                                                                                                                            >  1:57406/1‑139 (MQ=255)      ggTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGaa                                                                                                                        >  1:1042499/1‑139 (MQ=255)      ggTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGaa                                                                                                                        >  1:248592/1‑139 (MQ=255)           gCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAAtttt                                                                                                                   >  2:1168689/1‑139 (MQ=255)            cTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTg                                                                                                                  >  2:1131568/1‑139 (MQ=255)               ttACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTgcgc                                                                                                               >  2:1414198/1‑139 (MQ=255)                    aTCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGaa                                                                                                          >  2:302487/1‑139 (MQ=255)                      ctctcGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAg                                                                                                        <  1:1414198/139‑1 (MQ=255)                                           gTACATTCGACAGCATTTCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAgc                                                                                   >  1:1261634/1‑139 (MQ=255)                                           gTACATTCGACAGCATTTCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAgc                                                                                   <  2:1042499/139‑1 (MQ=255)                                             aCATTCGACAGCATTTCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAgcgc                                                                                 <  2:1406051/139‑1 (MQ=255)                                             aCATTCGACAGCATTTCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAgcgc                                                                                 <  1:302487/139‑1 (MQ=255)                                                        cATTTCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCAc                                                                      <  2:240605/139‑1 (MQ=255)                                                           ttCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCAcccc                                                                   <  1:1131568/139‑1 (MQ=255)                                                             cGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCAcccc                                                                   <  1:666456/137‑1 (MQ=255)                                                             cGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCAcccc                                                                   >  2:666456/1‑137 (MQ=255)                                                             cGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCAc                                                                 >  1:1282202/1‑139 (MQ=255)                                                                  cATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGt                                                            >  1:507758/1‑139 (MQ=255)                                                                    ttGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCg                                                          >  1:1158809/1‑139 (MQ=255)                                                                              ttCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACAACTGTTa                                                <  2:507758/139‑1 (MQ=255)                                                                               tctcTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACa                                                       >  2:150796/1‑131 (MQ=255)                                                                               tctcTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACa                                                       <  1:150796/131‑1 (MQ=255)                                                                               tctcTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACAACTGTTag                                               >  2:766150/1‑139 (MQ=255)                                                                                ctctGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACAACTGTTaga                                              <  2:248592/139‑1 (MQ=255)                                                                                ctctGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACAACTGTTaga                                              <  2:1282202/139‑1 (MQ=255)                                                                                 tctGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACAACTGTTagag                                             >  1:545339/1‑139 (MQ=255)                                                                                     gcgcGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGtt                                                              >  2:687341/1‑118 (MQ=255)                                                                                     gcgcGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGtt                                                              <  1:687341/118‑1 (MQ=255)                                                                                     gcgcGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACa                                                       >  1:1366979/1‑125 (MQ=255)                                                                                     gcgcGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACa                                                       <  2:1366979/125‑1 (MQ=255)                                                                                       gcgTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACAACTGTTAGAGGAatat                                       <  1:578626/139‑1 (MQ=255)                                                                                          tAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGt                                                                                       <  1:246396/88‑1 (MQ=255)                                                                                          tAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGt                                                                                       >  2:246396/1‑88 (MQ=255)                                                                                                cTTCCGCGTTTTTACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACAACTGTTAGAGGAATATGAGcagcag                              >  1:734276/1‑139 (MQ=255)                                                                                                         ttttACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGga                                                                                                 <  2:871318/63‑1 (MQ=255)                                                                                                         ttttACCTCCTTACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGga                                                                                                 >  1:871318/1‑63 (MQ=255)                                                                                                                   ttACTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACAACTGTTAGAGGAATATGAGCAGCAGGGCGGTTTTAACGAAacac           <  1:278147/139‑1 (MQ=255)                                                                                                                     aCTCCGCCTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACAACTGTTAGAGGAATATGAGCAGCAGGGCGGTTTTAACGAAAcacag         <  2:734276/139‑1 (MQ=255)                                                                                                                           ccTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACAACTGTTAGAGGAATATGAGCAGCAGGGCGGTTTTAACGAAAcacaggcacag   >  1:53791/1‑139 (MQ=255)                                                                                                                            cTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACa                                                       >  2:1417221/1‑86 (MQ=255)                                                                                                                            cTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACa                                                       <  1:1417221/86‑1 (MQ=255)                                                                                                                            cTTGGGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACAACTGTTAGAGGAATATGAGCAGCAGGGCGGTTTTAACGAAAcacaggcacagg  >  2:690239/1‑139 (MQ=255)                                                                                                                                |                                                                                                                                       TACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGATGCTTCTCTGGCGCGTAATCCCTTCCGCGTTTTTACCTCCTTACTCCGCCTTGAGCTTATCGAGAACGAAATTTTGCGCCAGAAAGCGGCGGAGATTCTGCGTCAGCGCGATATCTTCACCCCACGTTGTCGACAACTGTTAGAGGAATATGAGCAGCAGGGCGGTTTTAACGAAACACAGGCACAGG  >  NC_000913/1495561‑1495822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑